Soluble hiv-1 envelope glycoprotein trimers 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
EP2873423 B1 2017-05-31 [EP2873423]EP2873423 A3 2015-09-09 [EP2873423]EP2873423 A2 2015-05-20 [EP2873423] / 2017-05-312015-09-092015-05-20
申请号/申请日
2014EP-0003443 / 2014-10-07
发明人
WYATT RICHARD;WARD ANDREW;TRAN KAREN;KUMAR SHAILENDRA;INGALE JIDNYASA;GUENAGA JAVIER;FENG YU;DUBROVSKAYA VIKTORIYA;DE VAL ALDA NATALIA;
申请人
INT AIDS VACCINE INITIATIVE;SCRIPPS RESEARCH INSTITUTE;
主分类号
IPC分类号
A61K-039/21C07K-014/16
摘要
(EP2873423) The present application relates to novel HIV-1 envelope glycoproteins which may be utilized as an HIV-1 vaccine immunogens, antigens for crystallization and for the identification of broad neutralizing antibodies.  The present invention encompasses the preparation and purification of immunogenic compositions which are formulated into the vaccines of the present invention.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2013US-61887618 2013-10-07 2014US-61941101 2014-02-18 2014US-62032507 2014-08-01 2014US-62054727 2014-09-24
主权利要求
(EP2873423) 1. An engineered or non-naturally occurring JRFL SOSIP trimer. 2. The trimer of claim 1, wherein the trimer comprises (a) the amino acid sequence depicted in FIG. 5, or (b) the amino acid sequence depicted in FIG. 6 and cysteine mutations thereof, or (c) the amino acid sequence depicted in FIG. 7 and modifications thereof, or (d) the amino acid sequence of FIG. 8 and modifications thereof. 3. The trimer of claim 1, wherein the JRFL SOSIP trimer is truncated at residue 663 (JRFL SOSIP.663 trimer) and/or the JRFL SOSIP is stabilized by disulfide linkages, preferably at residues 201-433 (1 **st disulfide) and/or 163-309 (2 **nd disulfide). 4. An engineered or non-naturally occurring 16055 SOSIP trimer. 5. The trimer of claim 4, wherein the 16055 SOSIP trimer is truncated at residue 663 (16055 SOSIP.663 trimer). 6. An engineered or non-naturally occurring native flexible linker (NFL) gp140 trimer, wherein a linker covalently joins gp120 with gp41. 7. The trimer of claim 6, wherein the trimer comprises the schematic of FIG. 9. 8. The trimer of claim 6, wherein the trimer comprises (a) the amino acid sequence depicted in FIG. 10 or modifications thereof, or (b) the amino acid sequence depicted in FIG. 11 or modifications thereof, or (c) the amino acid sequence depicted in FIG. 12 or modifications thereof, or (d) the amino acid sequence depicted in FIG. 13 or modifications thereof, or (e) the amino acid sequence depicted in FIG. 14 or modifications thereof. 9. The trimer of claim 6, wherein the trimer is truncated following residue 664. 10. The trimer of any one of claims 6 to 9, wherein the trimer comprises a His tag. 11. The trimer of any one of claims 6 to 10, wherein the trimer is mutated. 12. The trimer of claim 11, wherein the mutation is a proline substitution. 13. The trimer of claim 11, wherein the mutation is selected from the group consisting of S649D, S649E, L555P, L556P, A558P and I559P, wherein the residues based on BG505 sequence and numbering, preferably wherein the mutation is I559P. 14. The trimer of claim 11, wherein the mutation is a double mutant containing combinations of 649D or E with the other HR1 P mutations. 15. The trimer of claim 11, wherein the mutation is selected from the group consisting of E47D, K49E, V65K, E106T, I165L, E429R, R432Q, wherein the residues based on BG505 sequence and numbering, or a subset thereof. 16. The trimer of claim 12, wherein the mutation is selected from the group consisting of L555P, Q652P, Q653P, L565P and L566P or any combination thereof, wherein the residues based on 16055 sequence and numbering. 17. The trimer of claim 12, wherein the mutation is a double mutant selected from the group consisting of 558P-S649D, A558P-S649E and I559P-S649E, wherein the residues based on 16055 sequence and numbering. 18. The trimer of any one of claims 6 to 10 wherein the trimer is a BG505 trimer or is a trimer homologous to BG505 from HIV subtypes A, B or C, wherein the trimer comprises any one of the sequences of FIG. 70 or 75 or a sequence having 95% identity thereof. 19. The trimer of any one of claims 6 to 10 comprising a free cysteine. 20. The trimer of claim 19, wherein the trimer is encoded by the sequence of FIG. 69. 21. The trimer of any one of claims 1 to 20 for use in a method of eliciting an immune response in a mammal. 22. The trimer of any one of claims 1 to 20 for the use of claim 21, wherein the trimer is administered with an adjuvant. 23. The trimer of any one of claims 1 to 20 for the use of claim 22, wherein the adjuvant comprises a lecithin, preferably wherein the adjuvant is a lecithin is combined with an acrylic polymer, a lecithin coated oil droplet in an oil-in-water emulsion or a lecithin and an acrylic polymer in an oil-in-water emulsion. 24. The trimer of any one of claims 1 to 20 for the use of claim 23, wherein the adjuvant is ISCOMATRIX or Adjuplex. 25. The trimer of any one of claims 1 to 20 for the use of claim 22, wherein the adjuvant comprises alum. 26. The trimer of any one of claims 1 to 20 for the use of any of claims 21 to 25, wherein the trimer is administered in a liposome or in a nanoparticle. 27. The trimer of any one of claims 1 to 20 for the use of any of claims 21 to 26, wherein the trimer is fixed, preferably wherein the trimer is fixed in glutaraldehyde.
法律状态
(EP2873423) LEGAL DETAILS FOR EP2873423  Actual or expected expiration date=2034-10-07    Legal state=ALIVE    Status=GRANTED     Event publication date=2014-10-07  Event code=EP/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=EP EP14003443  Application date=2014-10-07  Standardized application number=2014EP-0003443     Event publication date=2015-05-20  Event code=EP/A2  Event type=Examination events  Application published without search report  Publication country=EP  Publication number=EP2873423  Publication stage Code=A2  Publication date=2015-05-20  Standardized publication number=EP2873423     Event publication date=2015-05-20  Event code=EP/17P  Event indicator=Pos  Event type=Examination events  Request for examination filed Pruefungsantrag gestellt  Effective date of the event=2014-10-07     Event publication date=2015-05-20  Event code=EP/AK  Event indicator=Pos  Event type=Designated states  Designated contracting states: Benannte vertragsstaaten AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR    Event publication date=2015-05-20  Event code=EP/AX  Event indicator=Pos  Event type=Designated states  Extension of the European patent to Request for extension of the european patent to BA ME    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: FENG, YU    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: GUENAGA, JAVIER    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: KUMAR, SHAILENDRA    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: WARD, ANDREW    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: DE VAL ALTA, NATALIA    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: WYATT, RICHARD    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: TRAN, KAREN    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: INGALE, JIDNYASA    Event publication date=2015-08-05  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: DUBROWSKAYA, VIKTORIA    Event publication date=2015-09-09  Event code=EP/A3  Event indicator=Pos  Event type=Examination events  Published search report  Publication country=EP  Publication number=EP2873423  Publication stage Code=A3  Publication date=2015-09-09  Standardized publication number=EP2873423     Event publication date=2015-09-09  Event code=EP/AK  Event indicator=Pos  Event type=Designated states  Designated contracting states: Benannte vertragsstaaten AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR    Event publication date=2015-09-09  Event code=EP/AX  Event indicator=Pos  Event type=Designated states  Extension of the European patent to Request for extension of the european patent to BA ME    Event publication date=2015-09-09  Event code=EP/RIC1  Event type=Classification modifications  Classification (correction) Klassifikation (korr.) IPC: C07K  14/16        20060101ALI20150805BHEP    Event publication date=2015-09-09  Event code=EP/RIC1  Event type=Classification modifications  Classification (correction) Klassifikation (korr.) IPC: A61K  39/21        20060101AFI20150805BHEP    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: INGALE, JIDNYASA    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: GUENAGA, JAVIER    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: WARD, ANDREW    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: WYATT, RICHARD    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: FENG, YU    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: TRAN, KAREN    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: KUMAR, SHAILENDRA    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: DUBROVSKAYA, VIKTORIYA    Event publication date=2015-09-09  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: DE VAL ALTA, NATALIA    Event publication date=2016-03-09  Event code=EP/R17P  Event indicator=Pos  Event type=Administrative notifications  Request for examination filed (correction) Pruefungsantrag gestellt (korr.)  Effective date of the event=2016-01-21     Event publication date=2016-03-09  Event code=EP/RBV  Event indicator=Pos  Event type=Designated states  Event type=Corrections  Designated contracting states (correction): Benannte vertragsstaaten (korr.) AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR    Event publication date=2016-03-30  Event code=EP/RAP1  Event type=Change of name or address  Event type=Reassignment  Transfer of rights of an EP application Anmelder uebertragung (korr.) Owner: INTERNATIONAL AIDS VACCINE INITIATIVE    Event publication date=2016-03-30  Event code=EP/RAP1  Event type=Change of name or address  Event type=Reassignment  Transfer of rights of an EP application Anmelder uebertragung (korr.) Owner: THE SCRIPPS RESEARCH INSTITUTE    Event publication date=2016-08-03  Event code=EP/17Q  Event indicator=Pos  Event type=Examination events  First examination report Erster pruefungsbescheid  Effective date of the event=2016-06-29     Event publication date=2017-01-11  Event code=EP/INTG  Event indicator=Pos  Event type=Examination events  Announcement of intention to grant  Effective date of the event=2016-12-16     Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: INGALE, JIDNYASA    Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: GUENAGA, JAVIER    Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: WYATT, RICHARD    Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: DUBROVSKAYA, VIKTORIYA    Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: DE VAL ALDA, NATALIA    Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: FENG, YU    Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: TRAN, KAREN    Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: WARD, ANDREW    Event publication date=2017-03-29  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Erfinder (korr.) Inventor: KUMAR, SHAILENDRA    Event publication date=2017-05-31  Event code=EP/B1  Event indicator=Pos  Event type=Event indicating In Force  Patent specification  Publication country=EP  Publication number=EP2873423  Publication stage Code=B1  Publication date=2017-05-31  Standardized publication number=EP2873423
专利类型码
B1A3A2
国别省市代码
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