Recombinant chitinase and use thereof as a biocide 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
WO9708944 A1 1997-03-13 [WO9708944] / 1997-03-13
申请号/申请日
1996WO-US14440 / 1996-09-06
发明人
KRAMER KARL J;MUTHUKRISHNAN SUBBARATNAM;CHOI HEE KYUNG;CORPUZ LOLITA;GOPALAKRISHNAN BHUVANA;
申请人
KANSAS STATE UNIVERSITY;
主分类号
IPC分类号
A01N-063/00C12N-009/24C12N-015/12C12N-015/82
摘要
(WO9708944) A recombinantly derived insect chitinase gene is inserted into a vector for host expression of the chitinase protein, and may be utilized according to methods for controlling a population of pests (e.g., insects).  A recombinant virus is provided for this purpose, as well as a recombinant plant.  Recombinantly derived insect chitinase also may be expressed in host cells or larvae for subsequent purification.
机翻摘要
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地址
代理人
代理机构
;
优先权号
1995US-08524051 1995-09-06
主权利要求
(WO9708944) Claims: 1. A transgenic plant having a genome comprising a recombinantly derived genetic insert encoding an insect chitinase having pesticidal activity. 2. The plant of claim 1 , said pesticidal activity being insecticidal activity. 3. The plant of claim 1 , said chitinase having at least a 50% amino acid sequence homology to the protein encoded by Sequence ID No. 1. 4. The plant of claim 1 , said insert encoding the protein encoded by Sequence ID No. 1. 5. The plant of claim 1 , said insert comprising the coding region of Sequence ID No. 1. 6. The plant of claim 1 , said plant being tobacco or a cereal grain. 7. A method of controlling pests comprising the steps of: (a) growing a transgenic plant having a genome comprising a recombi¬ nantly derived genetic insert encoding an insect chitinase having pesticidal activity; and (b) allowing said pests to eat portions of said plant. 8. The method of claim 7, said pesticidal activity being insecticidal activity. 9. The method of claim 7, said pests being insects. 10. The method of claim 7, said chitinase having at least a 50% amino acid sequence homology to the protein encoded by Sequence ID No. 1. 11. The method of claim 7, said insert encoding the protein encoded by Sequence ID No. 1. 12. The method of claim 7, said insert comprising the coding region of Sequence ID No. 1. 13. The method of claim 7, said plant being tobacco or a cereal grain. 14. A method of controlling insect pests comprising the step of administering to said pests an insect chitinase having pesticidal activity. 15. The method of claim 14, said pesticidal activity being insecticidal activity. 16. The method of claim 14, said pests being insects. 17. The method of claim 14, said insect chitinase being orally administered to said pests. 18. The method of claim 14, said chitinase having at least a 50% amino acid sequence homology to the protein encoded by Sequence ID No. 1. 19. The method of claim 14, said insert encoding the protein encoded by Sequence ID No. 1. 20. The method of claim 14, said insert comprising the coding region of Sequence ID No. 1.
法律状态
(WO9708944) LEGAL DETAILS FOR WO9708944  Actual or expected expiration date=1999-03-06    Legal state=DEAD    Status=LAPSED     Event publication date=1996-09-06  Event code=WO/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=WO WOUS9614440  Application date=1996-09-06  Standardized application number=1996WO-US14440     Event publication date=1997-03-13  Event code=WO/A1  Event type=Examination events  Published application with search report  Publication country=WO  Publication number=WO9708944  Publication stage Code=A1  Publication date=1997-03-13  Standardized publication number=WO9708944     Event publication date=1997-03-13  Event code=WO/AK  Event indicator=Pos  Event type=Designated states  Designated states AU BR CA CN MX    Event publication date=1997-06-12  Event code=WO/DFPE  Event type=Examination events  Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)    Event publication date=1999-03-06  Event code=WO/EETL  Event type=Event indicating Not In Force  PCT Application validity period expired. LEGAL DETAILS FOR DESIGNATED STATE AU6971296  Actual or expected expiration date=2002-03-27    Legal state=DEAD    Status=LAPSED   Corresponding cc:  Designated or member state=AU Corresponding appl: AU6971296  Application date in the designated or member state=1996-09-06   Application number in the designated or member state=1996AU-0069712 Corresponding cc:  Designated or member state=AU Corresponding pat: AU6971296  Publication stage code in the designated or member state=A  Publication date in the designated or member state=1997-03-27   Publication number in the designated or member state=AU9669712    Event publication date=1997-03-27  Event code=AU/STCHG  Patent Status changed by the national office Corresponding cc:  Designated or member state=AU  LEGAL DETAILS FOR DESIGNATED STATE CA  Actual or expected expiration date=1999-03-06    Legal state=DEAD    Status=LAPSED   Corresponding cc:  Designated or member state=CA     Event publication date=1999-03-06  Event code=WO/NENP  Event type=Event indicating Not In Force  Non-entry into the national phase in: Corresponding cc:  Designated or member state=CA
专利类型码
A1
国别省市代码
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