一种​水稻​工程​保持​系​的​构建​方法​及其​应用

源语言标题
(CN104846009) 一种​水稻​工程​保持​系​的​构建​方法​及其​应用
公开号/公开日
CN104846009 A 2015-08-19 [CN104846009] / 2015-08-19
申请号/申请日
2015CN-0253259 / 2015-05-18
发明人
SONG SHUFENG; LI XINQI; LI LI; ZHANG DABING; FU XIQIN; YUAN DINGYANG; YUAN LONGPING
申请人
HUNAN HYBRID RICE RESEARCH CENTER
主分类号
IPC分类号
A01H-001/02;A01H-005/00;C12N-015/65;C12N-015/82
摘要
(CN104846009)
本​发明​公开​了​一种​水稻​工程​保持​系​的​构建​方法,包括:步骤​一、将​外源​报告​基因​定点​整合到​野生​型​水稻​的​染色体​上​得到​含有​外源​报告​基因​的​转基因​株系,其中,该​外源​报告​基因​与​野生​型​育​性​调控​基因​能够​紧密​连锁​遗传;以及,之后​进入​步骤​二,步骤​二、将​步骤​一中​得到​的​转基因​株系​与​纯​合​隐性​水稻​普通​雄性​核​不育​株系​杂交,筛选​含有​外源​报告​基因​的​株系​即为​所需​要的​水稻​工程​保持​系,其中,该​纯​合​隐性​水稻​普通​雄性​核​不育​株系​为​育​性​调控​基因突变​体​株系。本​发明​还​公开​了​水稻​工程​保持​系在​水稻​育种​中的​应用。本​发明​将​外源​报告​基因​定点​整合到​野生​型​水稻​的​染色体​上,解决​了​转化​受体​材料​受限制​的​问题。本​发明​的​水稻​工程​保持​系​可用​于​水稻​普通​隐性​雄性​核​不育​系​繁殖​中。
地址
代理人
代理机构
优先权号
2015CN-0253259 2015-05-18
主权利要求
(CN104846009)
1. A method for constructing the maintainer engineered rice, characterized in, comprising: Step one, the site-directed integration of the exogenous reporter gene on chromosome to wild-type rice containing the exogenous reporter gene transgenic lines, wherein, the exogenous reporter gene to the wild-type genetic control fertility closely linked gene can be; and, thereafter into the second step, Step two, transgenic lines obtained in step a the rice male sterile line with a homozygous recessive nuclear hybridizationn Normal, containing a foreign reporter transgene is the maintainer engineering desired rice, wherein, the rice male sterile line is homozygous recessive for the fertility regulation of a mutant nuclear common line.
法律状态
(CN104846009)
LEGAL DETAILS FOR CN104846009

Actual or expected expiration date=2035-05-18   
Legal state=ALIVE   
Status=PENDING 



Event publication date=2015-05-18 
Event code=CN/APP 
Event indicator=Pos 
Event type=Examination events 
Application details

Application country=CN CN201510253259 
Application date=2015-05-18 
Standardized application number=2015CN-0253259 



Event publication date=2015-08-19 
Event code=CN/A 
Event type=Examination events 
Published application

Publication country=CN 
Publication number=CN104846009 
Publication stage Code=A 
Publication date=2015-08-19 
Standardized publication number=CN104846009 



Event publication date=2015-08-19 
Event code=CN/C06 
Event indicator=Pos 
Event type=Examination events 
Publication



Event publication date=2015-09-16 
Event code=CN/EXSB 
Event type=Examination events 
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专利类型码
A
国别省市代码
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