Institute of Scientific Instruments of the CAS, v.v.i., Kralovopolska 147, 61264 Brno, Czech Republic osamek@isibrno.cz phone +420 5541 514 284 isibrno.czInstitute of Scientific Instruments of the CAS, v.v.i., Kralovopolska 147, 61264 Brno, Czech RepublicInstitute of Scientific Instruments of the CAS, v.v.i., Kralovopolska 147, 61264 Brno, Czech RepublicInstitute of Scientific Instruments of the CAS, v.v.i., Kralovopolska 147, 61264 Brno, Czech RepublicBrno University of Technology, Faculty of Chemistry, Purkyňova 118, 612 00 Brno, Czech RepublicInstitute of Scientific Instruments of the CAS, v.v.i., Kralovopolska 147, 61264 Brno, Czech RepublicInstitute of Scientific Instruments of the CAS, v.v.i., Kralovopolska 147, 61264 Brno, Czech RepublicSt. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech RepublicSt. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic
作者
Ota Samek;Zdenek Pilat;Silvie Bernatova;Jan Jezek;Tereza Klementova;Martin Kizovsky;Pavel Zemanek;Katarina Rebrosova;Filip Ruzicka
摘要
We report on the development of a set of Raman based techniques to monitor a large variety of biological and chemical analytes, such as various microorganisms, gels of hyaluronic acid and selected halogenated hydrocarbons using Raman spectroscopy, Raman tweezers and surface-enhanced Raman spectroscopy (SERS). We analyzed individual microbial cells with Raman tweezers to provide solutions for fast and label-free identification of specific bacterial or yeast species. We designed an optofluidic SERS platform for quantification of sub-millimolar concentrations of halogenated environmental pollutants such as 1,2,3-trichloropropane and chloroform. We also examined the gel structure of hyaluronic acid by Raman spectroscopy.
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