Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2001000175 A1 2001-04-05 [US20010000175]US6492121 B2 2002-12-10 [US6492121] / 2001-04-052002-12-10
申请号/申请日
2000US-09725265 / 2000-11-29
发明人
KURANE RYUICHIRO;KANAGAWA TAKAHIRO;KAMAGATA YOICHI;KURATA SHINYA;YAMADA KAZUTAKA;YOKOMAKU TOYOKAZU;KOYAMA OSAMU;FURUSHO KENTA;
申请人
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE & TECHNOLOGY;NIPPON STEEL KANKYO ENGINEERING;
主分类号
IPC分类号
C12Q-001/68
摘要
(US6492121) A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye.  The method comprises:providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid;hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
机翻摘要
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地址
代理人
代理机构
;
优先权号
1999JP-0111601 1999-04-20 2000US-09556127 2000-04-20 2000US-09725265 2000-11-29
主权利要求
(US6492121) What is claimed is: 1.  A method for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with only a fluorescent dye, which comprises: providing, as said probe, a nucleic acid probe capable of reducing fluorescence emission from said fluorescent dye when hybridized with said target nucleic acid; hybridizing said probe to said target nucleic acid; measuring a decrease in fluorescence emission from said fluorescent dye after said hybridization relative to fluorescence emission from said fluorescent dye before said hybridization, wherein only said nucleic acid probe is provided as a nucleic acid probe in the reaction system of the hybridization;  and correlating a rate of said decrease in fluorescence emission from said fluorescent dye after said hybridization to the concentration of the target nucleic acid. 2. The method according to claim 1, wherein said probe has G or C as a 3' end base and is labeled at said 3' end thereof with said fluorescent dye. 3. The method according to claim 1, wherein the nucleic acid probe has G or C as a 5' end base and is labeled at said 5' end thereof with said fluorescent dye. 4. The method according to claim 1, wherein a hydroxyl group of a 2' or 3' carbon of a ribose or a 3' carbon of a deoxyribose at 3' end of said probe has been phosphorylated. 5. The method according to claim 1, wherein an oligoribonucleotide of said probe is a chemically-modified nucleic acid. 6. The method according to claim 1, wherein an oligonucleotide of said probe is a chimeric oligonucleotide comprising a ribonucleotide and a deoxyribonucleotide. 7. The method according to claim 6, wherein said ribonucleotide is a 2'-O-methyloligoribonucleotide.
法律状态
(US6492121) LEGAL DETAILS FOR US2001000175  Actual or expected expiration date=2020-04-20    Legal state=ALIVE    Status=GRANTED     Event publication date=2000-11-29  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US09725265  Application date=2000-11-29  Standardized application number=2000US-09725265     Event publication date=2000-11-29  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: FREDMAN, JEFFREY NORMAN    Event publication date=2000-11-29  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1637     Event publication date=2000-11-29  Event code=US/ENT  Event type=Administrative notifications  Entity Status Set to Undiscounted Business Entity Status: UNDISCOUNTED    Event publication date=2000-11-29  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=2000-11-29  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: 199953US0XDIV    Event publication date=2000-11-29  Event code=US/CUST  Event type=Examination events  Attorney/Agent Customer Number Customer Nbr: 22850    Event publication date=2000-11-29  Event code=US/APE  Event type=Corrections  Preliminary amendments    Event publication date=2001-01-06  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=2001-03-21  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2001-04-05  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2001000175  Publication stage Code=A1  Publication date=2001-04-05  Standardized publication number=US20010000175     Event publication date=2001-05-23  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2001-11-01  Event code=US/EXIN  Event type=Examination events  Event type=OAO  Event type=INT  Examiner Interview Summary Record (PTOL - 413)    Event publication date=2001-11-26  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=2001-11-26  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=2002-01-04  Event code=US/CTFR  Event type=Examination events  Event type=OA  Event type=OAO  Final Rejection    Event publication date=2002-02-07  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=2002-04-04  Event code=US/CTFAR  Event type=Examination events  Event type=OAI  Response after Final Action    Event publication date=2002-05-02  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=2002-08-02  Event code=US/ANA  Event type=Corrections  Event type=OAI  Amendment after Notice of Allowance (Rule 312)    Event publication date=2002-08-06  Event code=US/APRDY  Event indicator=Pos  Event type=Examination events  Application Is Considered Ready for Issue    Event publication date=2002-10-31  Event code=US/N271  Event type=Examination events  Event type=OAO  Response to Amendment under Rule 312    Event publication date=2002-11-21  Event code=US/PAT  Event indicator=Pos  Event type=Event indicating In Force  Patented Case    Event publication date=2002-11-21  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Event type=Restitution or restoration  Issue Notification Mailed    Event publication date=2002-12-10  Event code=US/B2  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as second publication  Publication country=US  Publication number=US6492121  Publication stage Code=B2  Publication date=2002-12-10  Standardized publication number=US6492121     Event publication date=2002-12-10  Event code=US/354  Event indicator=Pos  Event type=Extension of term of duration of protection  Patent term extended under  35 U.S.C 154(b) until/for Delays (A,B,C): 0  Overlap Delays: 0  Non Overlap Delays: 8   PTO Office Delays: 0  Applicant Delays: 214  Adjustment total:  Number of days of extension=0    Event publication date=2002-12-12  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE  Effective date of the event=2001-04-01  ESTABLISHMENT OF INDEPENDENT ADMINISTRATIVE INSTITUTION BY JAPANESE GOVERNMENT ASSIGNOR:JAPAN AS REPRESENTED BY SECRETARY AND AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY REEL/FRAME:013563/0685     Event publication date=2003-01-28  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE  Effective date of the event=2002-12-17  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNOR:JAPAN BIOINDUSTRY ASSOCIATION REEL/FRAME:013705/0336     Event publication date=2003-01-28  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: KANKYO ENGINEERING CO., LTD., JAPAN  Effective date of the event=2002-12-17  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNOR:JAPAN BIOINDUSTRY ASSOCIATION REEL/FRAME:013705/0336     Event publication date=2005-12-20  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. PAYMENT NOTICE YEAR:  Year of payment of annual fees=3     Event publication date=2006-03-22  Event code=US/FPAY  Event indicator=Pos  Event type=Event indicating In Force  Event type=Payment or non-payment notifications  Fee payment recorded   Annual fees payment date=2006-03-22     Year of payment of annual fees=4     Event publication date=2009-11-12  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: NIPPON STEEL KANKYO ENGINEERING CO., LTD., JAPAN  Effective date of the event=2006-10-01  CHANGE OF NAME ASSIGNOR:KANKYO ENGINEERING CO., LTD. REEL/FRAME:023498/0693     Event publication date=2009-12-22  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. 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专利类型码
A1B2
国别省市代码
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