Fungicide residues exposure and β-amyloid aggregation in a mouse model of alzheimer’s disease 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
Environmental Health Perspectives
年/卷/期
Jan 2020 / 128 / 1
出版商
Public Health Services, US Dept of Health and Human Services
ISSN号
00916765
语言
English
作者
Lafon, Pierre-André 1 ; Wang, Yunyun 2 ; Arango-Lievano, Margarita 3 ; Torrent, Joan 1 ; Salvador-Prince, Lucie 1 ; Mansuy, Marine 1 ; Mestre-Francès, Nadine 1 ; Givalois, Laurent 1 ; Liu, Jianfeng 4 ; Mercader, Josep Vicent 5 ; Jeanneteau, Freddy 3 ; Desrumaux, Catherine 6 ; Perrier, Véronique 1 1 MMDN, University of Montpellier, EPHE, INSERM, Montpellier, France Veronique.perrier@umontpellier.fr 2 MMDN, University of Montpellier, EPHE, INSERM, Montpellier, France, Cellular Signaling Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China 3 Institut de Génomique Fonctionnelle, University of Montpellier, CNRS, INSERM, Montpellier, France 4 Cellular Signaling Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China 5 Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Paterna, València, Spain 6 MMDN, University of Montpellier, EPHE, INSERM, Montpellier, France, LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, Dijon, France
摘要
BACKGROUND: Pesticide residues have contaminated our environment and nutrition over the last century. Although these compounds are present at very low concentrations, their long-term effects on human health is of concern. The link between pesticide residues and Alzheimer’s disease is not clear and difficult to establish. To date, no in vivo experiments have yet modeled the impact of this chronic contamination on neurodegenerative disorders. OBJECTIVES: We investigated the impact of fungicide residues on the pathological markers of Alzheimer’s disease in a transgenic mouse model. METHODS: Transgenic (J20, hAPPSw/Ind ) mice were chronically exposed to a cocktail of residues of cyprodinil, mepanipyrim, and pyrimethanil at 0:1 μg/L in their drinking water for 9 months. We assessed the effects of fungicide residues on the pathological markers of the disease including Aβ aggregates, neuroinflammation, and neuronal loss. Then, we studied the dynamics of Aβ aggregation in vivo via a longitudinal study using two-photon microscopy. Finally, we investigated the molecular mechanisms involved in the production and clearance of Aβ peptides. RESULTS: We found that a chronic exposure to three fungicide residues exacerbated aggregation, microgliosis, and neuronal loss. These fungicides also increased vascular amyloid aggregates reminiscent of cerebral amyloid angiopathy between 6 and 9 months of treatment. The mechanism of action revealed that fungicides promoted Aβ peptide fibril formation in vitro and involved an in vivo overexpression of the levels of the β-secretase– cleaving enzyme (BACE1) combined with impairment of Aβ clearance through neprylisin (NEP). CONCLUSIONS: Chronic exposure of the J20 mouse model of Alzheimer’s disease to a cocktail of fungicides, at the regulatory concentration allowed in tap water (0:1 μg/L), strengthened the preexisting pathological markers: neuroinflammation, Aβ aggregation, and APP β-processing. We hypothesize prevention strategies toward pesticide long-term exposure may be an alternative to counterbalance the lack of treatment and to slow down the worldwide Alzheimer’s epidemic. https://doi.org/10.1289/EHP5550.
机翻摘要
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关键词/主题词
Alzheimer disease (major);amyloid beta protein (major);animal experiment;animal model;animal tissue;Article;beta secretase;chemical structure;controlled study;cyprodinil;cytokine;degenerative disease;enzyme assay;fibril;fluorescence microscopy;fungicide (major);gene expression;gene overexpression;genotype;gliosis;hippocampus;human;immunoblotting;immunohistochemistry;interleukin derivative;male;mepanipyrim +;mouse;nervous system inflammation;nonhuman;optical coherence tomography;priority journal;protein aggregation (major);pyrimethanil +;real time polymerase chain reaction;reverse transcription polymerase chain reaction;RNA extraction;transgenic mouse;unclassified drug;upregulation;vascular amyloidosis;Western blotting
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