The study aims to investigate the construction and efficacy of a 3D-printed antibiotic scaffold through the combination of vancomycin-loaded polymethyl methacrylate with a 3D-printed porous titanium scaffold. The titanium scaffolds were constructed with orthogonal micro-structures usingselective laser melting and the micro-pores were filled with vancomycin-loaded polymethyl methacrylate while the polymethyl methacrylate was in its liquid phase. Structural features, the release of antibiotics, and the antibacterial performance of vancomycin-loaded polymethyl methacrylate-titaniumscaffolds were measured and compared with a titanium-free control group. Tests were also conducted to determine the loading force, deformation, and maximum pressure that the three different scaffolds could withstand. The results demonstrate that vancomycin-loaded polymethyl methacrylate maypermeate into the pores of a 3D-printed titanium scaffold, which allows the vancomycin-loaded polymethyl methacrylate-titanium scaffold to remain active over a longer period and have a stronger effect on<i> Staphylococcus aureus populations than a vancomycin-loaded polymethyl methacrylateblock (<i>P < 0.05). The vancomycin-loaded polymethyl methacrylate-titanium scaffold also has greater biomechanical strength than its constituents. These results suggest that selective laser meltingis a promising technique for producing porous 3D-printedtitanium scaffolds and that maybe efficient and reliable in the treatment of bone infection when impregnated with vancomycin-loaded polymethyl methacrylate.