In this paper, the influence of clay amendment on antibiotic resistance genes (ARGs) fate and their relation with bacterial diversity in poultry manure (PM) composting was evaluated. The results confirmed that 4% clay amendment was significantly reduce the relative abundances (RAs) 94% of ARGs, while higher dosage of clay blended treatments (T5 and T6) composts were observed relatively very high percentage of ARGs as compared to other clay blended treatments. Clay addition play potential role on the occurrence and distribution of ARGs during the composting by altering the overall RAs of Firmicutes and Proteobacteria, and there host bacterial species. The heat map correlation figure also indicated that reduction of ARGs was significantly associated with the alteration of microbial dynamics and physicochemical parameters during the composting. Therefore, the addition of 4% clay in to PM composting system could efficiently mitigate the greater concentration of ARGs in PM compost and also environmental risk associated with its application.