Methods and compositions for the simultaneous control of root diseases 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
CA2245504 A1 1997-08-07 [CA2245504] / 1997-08-07
申请号/申请日
1997CA-2245504 / 1997-02-03
发明人
THOMASHOW LINDA S;KIM DAL-SOO;WELLER DAVID M;COOK R JAMES;
申请人
UNIVERSITY OF WASHINGTON;US DEPARTMENT OF AGRICULTURE;
主分类号
IPC分类号
A01N-063/00
摘要
(CA2245504) Methods and products useful to simultaneously control the root diseases caused by Gaeumannomyces graminis, Rhizoctonia, and Pythium in crops susceptible to these root diseases are described.  These include a method to identify seedtreatment products for potential to simultaneously control the three kinds of root diseases; Bacillus strains having activity against the three kinds of root diseases and methods of obtaining the same, and compositions comprising a fungicide and a Bacillus strain having activity against the three kinds of root diseases, which compositions are useful for field control of the combination of the three kinds of root diseases.
机翻摘要
暂无翻译结果,您可以尝试点击头部的翻译按钮。
地址
代理人
代理机构
;
优先权号
1996US-60011192 1996-02-05 1997US-08788604 1997-01-24
主权利要求
(CA2245504)  What is claimed is: 1. A method for screening bacteria for selection of Bacillus strains having activity against the root diseases caused by Gaeumannomyces graminis, Rhizoctonia, and Pythium, which comprises: (1) isolating root-associated Bacillus spp. as follows: (a) collecting roots from seedlings or stubble from fields cropped continuously for at least three years to a crop susceptible to the targeted root diseases;  (b) washing the roots in sterile water or suitable buffer at 4[deg.]C for a number of washing sufficient to remove material not tightly adhering to the roots;  (c) transferring the roots to fresh sterile water or buffer and heating for a time and at a temperature sufficient to kill bacteria unable to form spores;  (d) macerating the roots, wherein said macerating may be carried out before or after said heating of said roots;  (e) obtaining pure isolates by making up the heat-treated root macerates to a known volume with buffer, dilution plating on a suitable medium to isolate individual colonies, and restreaking the individual colonies on a suitable medium to obtain pure isolates;  (2) screening the strains isolated in step (1)(e) for inhibitory activity in vitro against Rhizoctonia, Gaeumannomyces graminis and Pythium as follows: (a) first, screening the strains isolated in step (1)(e) for inhibitory activity in vitro against Rhizoctonia by growing a test strain on a suitable medium in the presence of Rhizoctonia, and selecting those strains that show inhibitory activity as measured by a zone of inhibition between the test strain and Rhizoctonia;  (b) screening the strains selected in step (2)(a) for inhibitory activity in vitro against Gaeumannomyces graminis and Pythium individually, in any order, by growing said test strain on a suitable medium in the 48 presence of Gaeumannomyces graminis and selecting those strains that show a zone of inhibition between the test strain and Gaeumannomyces graminis, and growing said test strain on a suitable medium in the presence of Pythium, and selecting those strains that show a zone of inhibition between the test strain and Pythium;  (3) screening the strains selected in step (2)(b) for biocontrol activity in the greenhouse or growth chamber against Rhizoctonia, Gaeumannomyces graminis and Pythium as follows: (a) first, screening the strains for biocontrol activity against Rhizoctonia by growing seeds of a cereal or grain legume crop treated with a test strain, in soil in the greenhouse or growth chamber in the presence of Rhizoctonia, and selecting those strains that show biocontrol activity as measured by reduction of Rhizoctonia root rot, or by increases in seedling emergence, plant height, first true leaf or other standard measure compared to control plants grown under the same conditions from seeds not treated with the test strain;  (b) screening the strains selected in step (3)(a) for biocontrol activity against Gaeumannomyces graminis and Pythium individually, in any order, by growing seeds of said cereal or grain legume crop treated with said test strain in soil in the greenhouse or growth chamber in the presence of Gaeumannomyces graminis, and selecting those strains that show biocontrol activity as measured by reduction of take-all or by increases in seedling emergence, plant height, length of first true leaf, or other standard measure compared to control plants grown under the same conditions from seeds without the test strain, and growing seeds treated with said test strain in soil in the greenhouse or growth chamber in the presence of Pythium, and selecting those strains that show biocontrol activity as measured by reduction of Pythium root rot or by increases in seedling emergence, plant height, length of first true leaf, or other 49 standard measure compared to control plants grown under the same conditions from seeds not treated with the test strain. 2. A biologically pure strain of Bacillus which has activity against the root diseases caused by Gaeumannomyces graminis, Rhizoctonia, and Pythium as determined by passing the screen test of claim 1. 3. The biologically pure strain of Bacillus of claim 2 further identified as having the identifying characteristics of NRRL B-21525. 4. A biocontrol composition useful for field control of the root diseases caused by Gaeumannomyces graminis, Rhizoctonia, and Pythium, which comprises a chemical fungicide and a biologically pure strain of Bacillus sp. which has activity against the root diseases as determined by passing the screen test of claim 1. 5. The biocontrol composition of claim 4 wherein said biological pure strain of Bacillus has the identifying characteristics of NRRL B-21525. 6. The biocontrol composition of claim 4 wherein said chemical fungicide is difenoconazole. 7. A product comprising a seed of a cereal or legume grain crop coated with a biologically pure strain of Bacillus which has activity against the root diseases caused by Gaeumannomyces graminis, Rhizoctonia, and Pythium as determined by passing the screen test of claim 1, the concentration of said strain being about 10 4 to 10 8 colony forming units per seed. 8. The product of claim 7 which further includes a chemical fungicide. 9. A method of controlling the root diseases caused by Gaeumannomyces graminis, Rhizoctonia, and Pythium in crops susceptible to said root diseases, which comprises treating seed of said crop with a composition comprising an effective amount of a biologically pure strain of Bacillus which has activity against the root diseases as determined by passing the screen test of claim 1. 10. The method of claim 9 wherein said composition further includes an effective amount of a chemical fungicide. 11. The method of claim 9 wherein said biologically pure strain of Bacillus has the identifying characteristics of NRRL B-21525. 12. The method of claim 10 wherein said chemical fungicide is difenoconazole. 13. A biologically pure strain of Bacillus that (a) has the ability to grow at 4[deg.]C, (b) has the ability to grow on the roots of cereal or legume grain crops, (c) is inhibitory in vitro against Rhizoctonia, Gaeumannomyces graminis and Pythium, and (d) has the ability to suppress root disease caused by Rhizoctonia, Gaeumannomyces graminis and Pythium in plants grown from seeds treated with said strain and grown in the presence of Rhizoctonia, Gaeumannomyces graminis and Pythium in the greenhouse or growth compared to plants grown under the same conditions from seed not treated with said strain. 14. A method for identifying seed-treatment products for potential to simultaneously control the root diseases caused by the pathogens Gaeumannomyces graminis, Rhizoctonia, and Pythium, which comprises planting seeds treated with a test seed-treatment product into field soil with minimal soil disruption, said soil having populations of Gaeumannomyces graminis, Rhizoctonia, and Pythium sufficient to provide substantially equal and simultaneous pressure of the pathogens, 51 and identifying said seed-treatment products having potential to simultaneously control the root diseases caused by the pathogens as measured by increased growth or decreased root disease in plants grown from said treated seeds compared to plants grown under the same conditions from seed not having the test seed treatment. 15. The method of claim 14 wherein said field soil has a pH of about 5 to 6, a clay content of less than about 20%, a soil temperature ranging from about 5 to 15[deg.]C, and a soil moisture status near field capacity. 52
法律状态
(CA2245504) LEGAL DETAILS FOR CA2245504  Actual or expected expiration date=2009-02-03    Legal state=DEAD    Status=LAPSED     Event publication date=1997-02-03  Event code=CA/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=CA CA2245504  Application date=1997-02-03  Standardized application number=1997CA-2245504     Event publication date=1997-08-07  Event code=CA/A1  Event type=Examination events  Application laid open  Publication country=CA  Publication number=CA2245504  Publication stage Code=A1  Publication date=1997-08-07  Standardized publication number=CA2245504     Event publication date=2002-01-29  Event code=CA/EEER  Event indicator=Pos  Event type=Examination events  Examination request    Event publication date=2009-02-03  Event code=CA/FZDE  Event indicator=Neg  Event type=Event indicating Not In Force  Dead
专利类型码
A1
国别省市代码
若您需要申请原文,请登录。

最新评论

暂无评论。

登录后可以发表评论

意见反馈
返回顶部