Method for producing porous cellulose beads using alkali aqueous solution, carrier for ligand immobilization, and adsorbent 机翻标题: 暂无翻译,请尝试点击翻译按钮。

源语言标题
(WO201546473) アルカリ​水溶液​を​用い​た​多孔​質​セルロース​ビーズ​の​製造​方法、リガンド​固定​化​用​担体​および​吸着​体
公开号/公开日
WO2015046473 A1 2015-04-02 [WO201546473] / 2015-04-02
申请号/申请日
2014WO-JP75740 / 2014-09-26
发明人
OKUBO TAKAHIRO;KAWAI YOSHIKAZU;HIRANO MASARU;KONOIKE FUMINORI;KARASUGI KEIICHI;HONDA TATSUYA;
申请人
KANEKA;
主分类号
IPC分类号
B01D-015/08B01J-020/24C07K-001/22C08B-001/08C08B-015/08C08B-015/10C08B-037/12G01N-030/88
摘要
(WO201546473)  This method for producing porous cellulose beads is characterized in that a cellulose slurry is made by adding b) water to a) a cellulose microdispersion produced by mixing a low-temperature alkali aqueous solution and cellulose, and the slurry is then brought into contact with d) a coagulation solvent.  This carrier for ligand immobilization is obtained by shrinking polysaccharide porous beads 10% or more by a shrinkage rate determined by the formula, and crosslinking.  Shrinkage rate (%) = (1-V2/V1)×100) (V1 is the bead volume before shrinkage, V2 is the bead volume after shrinkage)
机翻摘要
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地址
代理人
代理机构
;
优先权号
2013JP-0202007 2013-09-27 2013JP-0211453 2013-10-08
主权利要求
(WO201546473)  Claims machine translated from Japanese 1. A) Mixing with the alkaline aqueous solution and the cellulose, in the cellulose differential scattering liquid of the low temperature which it produces,  b) After making cellulose slurry including the water,  d) It features that it makes the solidification solvent contact, production method of the porous cellulose beads. 2. The description above b) After the process,  c) Cellulose slurry is heated,  After that, the description above d) in the claim 1 which does process production method of statement. 3. Alkaline density of the cellulose differential scattering liquid is below 10wt% above 8wt%, in claim 1 or 2 production method of statement. 4. Alkaline density of cellulose slurry is above 5wt%, either of the claim 1-3 in one section production method of statement. 5. The temperature which produces cellulose slurry is below the 20.deg.C above the 4.deg.C, either of the claim 1-4 in one section production method of the cellulose porous beads of statement. 6. The liquid - the liquid it disperses the aforementioned cellulose slurry in the non water soluble liquid which is dispersion medium and after producing the liquid drop, the said liquid - it features that the liquid dispersed liquid is made to contact the solidification solvent, either of the claim 1-5 in one section production method of the porous cellulose beads of statement. 7. It features that density of the cellulose of the aforementioned cellulose slurry is 1-7wt%, either of the claim 1-6 in one section production method of the cellulose beads of statement. 8. It features that the aforementioned cellulose is the regenerated cellulose, the crystal characteristic cellulose, the crystallite characteristic cellulose or cellulose acetate, either of the claim 1-7 in one section production method of the porous cellulose beads of statement. 9. It features that the degree of polymerization of the aforementioned cellulose is 1000 or less, in claim 8 production method of the porous cellulose beads of statement. 10. The aforementioned non water soluble liquid dichlorobenzene, Hexan, ethyl acetate, the direct condition saturated fatty acid of the carbon count 6-12 and the unsaturated fatty acid of the carbon count 16-24, fusion point the flora and fauna oils and fats below the 100.deg.C, the hydrogenation flora and fauna oil, the division oil which it divided refined high fusion point fraction of the flora and fauna oils and fats and that hydrogen additive, saturated fatty acid triglycerides, for food wax, the minute seaweed origin oils and fats and the microbial oils and fats, features that they are medium chain fatty acid triglycerides or unsaturated fatty acid triglycerides, either of the claim 1-9 in one section production method of the porous cellulose beads of statement. 11. The aforementioned solidification solvent alcohol or features that glycol is contained, either of the claim 1-9 in one section production method of the porous cellulose beads of statement. 12. The aforementioned alcohol the isobutanol, 2 - butanol and [sec]-butanoru, 2 - methyl - 2 - propanol, 1 - propanol, 2 - features that they are 1 kinds or more which are selected from the group which consists of propanol, the ethanol and methanol, in claim 11 production method of the porous cellulose beads of statement. 13. It features that they are 1 kinds or more which are selected from the group where the aforementioned glycol consists of the glycerine, ethylene glycol and propylene glycol, in claim 11 production method of the porous cellulose beads of statement. 14. The removal limited molecular weight the 1.0x10(6)-1.0x10 (11) either of the claim 1-13 which features that is the porous cellulose beads which in one section are obtained with production method of statement. 15. In the claim 14 which features that median particle diameter is 100 .micro.m from 50 .micro.m the porous cellulose beads of statement. 16. The carrier for ligand fixation which can by 10% or more contracting the polysaccharide porous beads in the coefficient of contraction which becomes settled due to the below-mentioned formula builds a bridge.  Coefficient of contraction (%) = (1-V [2] /V [1]) x100   (In formula, V [1] shows the gel cubic measure of the polysaccharide porous beads before the contracting, V [2] shows the gel cubic measure of the polysaccharide porous beads after the contracting.) 17. The polysaccharide porous beads passing the water soluble organic solvent and the contractile process which is made to contact with the alkaline water, in the claim 16 which is produced the carrier for ligand fixation of statement. 18. While coexisting the building a bridge medicine with the aforementioned contractile process, contracting it is produced by building a bridge, or  After the description above contractile process, the contractile beads which are obtained executing the building a bridge process which builds a bridge depending, in the claim 17 which is produced the carrier for ligand fixation of statement. 19. Vis-a-vis the beads which can by the aforementioned building a bridge, the said beads the building a bridge medicine and the additional building a bridge process which is made to contact with the alkaline water in the claim 18 which 1 times or more is executed the carrier for ligand fixation of statement. 20. In either of the claim 16-19 where the aforementioned polysaccharide is cellulose or [agarosu] the carrier for ligand fixation of statement. 21. The aforementioned water soluble organic solvent, in either of the claim 17-20 which at least is 1 where it is selected from the alcoholic solvent, the sulfoxide solvent, the amide solvent, the ketone solvent, and the ether solvent kinds the carrier for ligand fixation of statement. 22. In either of the claim 19-21 which does not use the alcoholic solvent with additional building a bridge process the carrier for ligand fixation of statement. 23. The adsorbent which in either of the claim 16-22 fixes the ligand in the carrier of statement. 24. In the claim 23 where the aforementioned ligand is [ahuiniteirigando] the adsorbent of statement. 25. Aforementioned [ahuiniteirigando], in the claim 24 which is protein A, protein G or protein L the adsorbent of statement. 26. Being refinement method of the antibody due to [ahuiniteikuromatogurahui], making the supply raw materials the adsorbent of statement contact, in claim, 24 or 25 including the stage where it makes the antibody adsorb, adds the effluent which separates the antibody from stage and the adsorbent which wash the antibody which adsorbs into the adsorbent appropriately, collects the antibody from the effluent, the method of becoming.
法律状态
(WO201546473) LEGAL DETAILS FOR WO2015046473  Actual or expected expiration date=2017-03-27    Legal state=DEAD    Status=LAPSED     Event publication date=2014-09-26  Event code=WO/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=WO WOJP2014075740  Application date=2014-09-26  Standardized application number=2014WO-JP75740     Event publication date=2015-04-02  Event code=WO/A1  Event type=Examination events  Published application with search report  Publication country=WO  Publication number=WO2015046473  Publication stage Code=A1  Publication date=2015-04-02  Standardized publication number=WO201546473     Event publication date=2017-03-27  Event code=WO/EETL  Event type=Event indicating Not In Force  PCT Application validity period expired. LEGAL DETAILS FOR DESIGNATED STATE DE  Actual or expected expiration date=2016-03-29    Legal state=DEAD    Status=LAPSED   Corresponding cc:  Designated or member state=DE     Event publication date=2016-03-29  Event code=WO/NENP  Event type=Event indicating Not In Force  Non-entry into the national phase in: Corresponding cc:  Designated or member state=DE  LEGAL DETAILS FOR DESIGNATED STATE EP3050902  Actual or expected expiration date=2034-09-26    Legal state=ALIVE    Status=PENDING   Corresponding cc:  Designated or member state=EP Corresponding appl: EP14849556  Application date in the designated or member state=2014-09-26   Application number in the designated or member state=2014EP-0849556 Corresponding cc:  Designated or member state=EP Corresponding pat: EP3050902  Publication stage code in the designated or member state=A1  Publication date in the designated or member state=2016-08-03   Publication number in the designated or member state=EP3050902    Event publication date=2015-05-20  Event code=WO/121  Event type=Designated states  EP: The EPO has been informed by wipo that ep was designated in this application Corresponding cc:  Designated or member state=EP  LEGAL DETAILS FOR DESIGNATED STATE JPWO2015046473  Actual or expected expiration date=2034-09-26    Legal state=ALIVE    Status=PENDING   Corresponding cc:  Designated or member state=JP Corresponding appl: JP2015539401  Application date in the designated or member state=2014-09-26   Corresponding cc:  Designated or member state=JP Corresponding pat: JPWO2015046473  Publication stage code in the designated or member state=A1  Publication date in the designated or member state=2017-03-09   Publication number in the designated or member state=JP2015046473W    Event publication date=2016-03-11  Event code=WO/ENP  Event type=Entry into national phase  Entry into the national phase in: Corresponding cc:  Designated or member state=JP  LEGAL DETAILS FOR DESIGNATED STATE US2016244483  Actual or expected expiration date=2034-09-26    Legal state=ALIVE    Status=PENDING   Corresponding cc:  Designated or member state=US Corresponding appl: US15025152  Application date in the designated or member state=2014-09-26   Application number in the designated or member state=2014US-15025152 Corresponding cc:  Designated or member state=US Corresponding pat: US2016244483  Publication stage code in the designated or member state=A1  Publication date in the designated or member state=2016-08-25   Publication number in the designated or member state=US20160244483
专利类型码
A1
国别省市代码
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