Wound-on-a-chip: High-throughput 3D wound healing assay with a novel SU-8 mesh chip 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
Sensors and Actuators, B. Chemical
年/卷/期
2019 / 280 /
页码
86-93
ISSN号
0925-4005
作者单位
Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Hubei Bioinformat & Mol Imaging Key Lab,Coll Life, Wuhan Natl Lab Optoelect,Syst Biol Theme,Dept Bio, Wuhan 430074, Hubei, Peoples R China;Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Hubei Bioinformat & Mol Imaging Key Lab,Coll Life, Wuhan Natl Lab Optoelect,Syst Biol Theme,Dept Bio, Wuhan 430074, Hubei, Peoples R China;Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Hubei Bioinformat & Mol Imaging Key Lab,Coll Life, Wuhan Natl Lab Optoelect,Syst Biol Theme,Dept Bio, Wuhan 430074, Hubei, Peoples R China;Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Hubei Bioinformat & Mol Imaging Key Lab,Coll Life, Wuhan Natl Lab Optoelect,Syst Biol Theme,Dept Bio, Wuhan 430074, Hubei, Peoples R China;Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Hubei Bioinformat & Mol Imaging Key Lab,Coll Life, Wuhan Natl Lab Optoelect,Syst Biol Theme,Dept Bio, Wuhan 430074, Hubei, Peoples R China;Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Hubei Bioinformat & Mol Imaging Key Lab,Coll Life, Wuhan Natl Lab Optoelect,Syst Biol Theme,Dept Bio, Wuhan 430074, Hubei, Peoples R China;Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Hubei Bioinformat & Mol Imaging Key Lab,Coll Life, Wuhan Natl Lab Optoelect,Syst Biol Theme,Dept Bio, Wuhan 430074, Hubei, Peoples R China;Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Hubei Bioinformat & Mol Imaging Key Lab,Coll Life, Wuhan Natl Lab Optoelect,Syst Biol Theme,Dept Bio, Wuhan 430074, Hubei, Peoples R China;
作者
Zhu, Jinchi;Wang, Yachao;Chen, Peng;Li, Yiwei;Yan, Shuangqian;Wang, Jie;Du, Wei;Liu, Bi-Feng;
摘要
The dynamic process of cells is the basis of many biological problems in living organisms and has been a major research subject over several decades. In this paper, we present a novel SU-8 mesh chip (SMC) with thousands of chamber arrays for high-throughput 3D wound healing assay, which could directly and efficiently evaluate 3D cell growth process and cellular drug responses in vitro. Four kinds of adherent cells (HeLa, HepG2, HUVEC, NIH-3T3) were successfully seeded into the SMC to form three-dimensional hollow cell aggregates models with precisely pre-defined sizes and shapes, followed by monitored their spontaneous growth process in real time. Compared with traditional 2D wound healing assay, this 3D model provided more complex cellular processes and some tissue-specific properties, which conferred a high degree of clinical and biological relevance to in vitro models. NIH-3T3 and HUVEC cell aggregates in this chip that termed as "wound on a chip" model had been developed and further investigated the drug response in these 3D wound healing processes by applying different concentrations of candidate drugs.
机翻摘要
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关键词/主题词
SU-8 mesh chip;3D cell culture;Wound-on-a-chip;Wound healing;
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