Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
Materials Science & Engineering, C. Materials for biological applications
年/卷/期
2018 / 90 / Sep.
页码
494-503
ISSN号
0928-4931
作者单位
School of Biotechnology, Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, Himachal Pradesh, 173229, India;College of Materials Science and Engineering, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, Nanshan District Key Lab for Biopolymers and Safety Evaluation, Shenzhe;School of Biotechnology, Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, Himachal Pradesh, 173229, India;School of Biotechnology, Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, Himachal Pradesh, 173229, India;School of Biotechnology, Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, Himachal Pradesh, 173229, India;
作者
Indu Hira;Amit Kumar;Reena Kumari;Adesh K. Saini;Reena V. Saini;
摘要
Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite was prepared by precipitation technique. The composite was characterized by using FT-IR, XRD, HRTEM, SAED, EDS, and SEM. TEM images showed the hexagonal shape of nanocomposite with the size range of 50-70 nm. Further, PEC-GG-ZnO was used as an im-munomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL). The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml). ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment. Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL. The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death. Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
机翻摘要
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关键词/主题词
Immunotherapy;Nanocomposite;Anticancer;Pectin;Guar gum;
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