Photoactivable Polymers Embedded with Cadmium-Free Quantum Dots and Crystal Violet: Efficient Bactericidal Activity against Clinical Strains of Antibiotic-Resistant Bacteria 机翻标题: 暂无翻译,请尝试点击翻译按钮。

ACS applied materials & interfaces
2019 / 11 / 13
UCL, Dept Chem, Mat Chem Res Ctr, 20 Gordon St, London WC1H 0AJ, England;UCL, UCL Eastman Dent Inst, Dept Microbial Dis, 256 Grays Inn Rd, London WC1X 8LD, England;UCL, UCL Div Surg & Intervent Sci, Charles Bell House,43-45 Foley St, London W1W 7TS, England;UCL, UCL Div Surg & Intervent Sci, Charles Bell House,43-45 Foley St, London W1W 7TS, England;UCL, UCL Div Surg & Intervent Sci, Charles Bell House,43-45 Foley St, London W1W 7TS, England;Nanoco Technol Ltd, 46 Grafton St, Manchester M13 9NT, Lancs, England;
Parkin, Ivan P.;Allan, Elaine;Yaghini, Elnaz;Owusu, Ethel G. A.;MacRobert, Alexander J.;Naasani, Imad;
The rising incidence of antibiotic-resistant infections from contaminated surfaces in hospitals or implanted medical devices has led to increasing interest in new antibacterial surfaces. Photoactivatable surfaces that can generate cytotoxic reactive oxygen species under exposure to ambient light is a promising approach to inactivation of surface-borne microorganisms. There is growing interest in the use of quantum dots (QDs) as light-harvesting agents for photobactericidal applications, but the cadmium in commonly used QDs will restrict clinical application. Herein, the photobactericidal activity of novel polyurethane substrates containing cadmium-free QDs was tested against clinical multidrug-resistant Gram-positive and Gram-negative bacterial strains: methicillin-resistant Staphylococcus aureus (MRSA) and a carbapenemase-producing strain of Escherichia coli (E. coli). To enhance the capacity for reactive oxygen species generation, QDs were incorporated into the polymer with a photosensitizing dye, crystal violet. Close proximity between the QD and dye enables electron and energy transfer processes leading to generation of cytotoxic singlet oxygen and superoxide radicals. A QD solution in cyclohexane was premixed with a solution of CV in the more polar solvent, dichloromethane, to promote the formation of QD-CV nanocomposite complexes via CV adsorption. This solution was then used to embed the QDs and crystal violet into medical grade polyurethane via swell encapsulation. The combination of QD and CV elicited significant synergistic antibacterial activity under visible light against MRSA within 1 h (99.98% reduction) and E. coli within 4 h (99.96% reduction). Photoluminescence lifetime and singlet oxygen phosphorescence measurements demonstrated that interaction between the QDs and the crystal violet occurs within the polymer and leads to enhanced generation of reactive oxygen species. Strong inhibition of kill was observed using the superoxide scavenger, superoxide dismutase. The efficacy of these QD CV polymer substrates, that can harvest light across the visible spectrum, against multidrug-resistant bacteria demonstrates the feasibility of this approach.
antibacterial;surfaces;polymer;light-activated;photodynamic therapy;quantum dots;crystal violet;reactive oxygen species;