METHOD FOR PRODUCING NEMATOCIDAL COMPOSITION BY HEAT TREATING A pH-ADJUSTED FERMENTATION BROTH 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
WO200176612 A1 2001-10-18 [WO200176612]WO200176612 A8 2002-06-20 [WO200176612] / 2001-10-182002-06-20
申请号/申请日
2001WO-US11699 / 2001-04-10
发明人
WARRIOR PREM;HEIMAN DANIEL F;REHBERGER LINDA A;JOHNSON RONALD E;HANSEN JAMES R;MCVICKER KEVIN A;
申请人
VALENT BIOSCIENCES;
主分类号
IPC分类号
A01N-061/00A01N-063/00A01N-063/02A61K-035/66A61K-035/74A61K-036/06A61K-036/062C12N-001/20C12P-001/00C12P-001/02C12P-001/04C12P-001/06
摘要
(WO200176612) The present invention is directed to a method for producing a new nematocidal composition particularly useful against plant parasitic nematodes and also a process to prevent damage resulting from nematode infestation.  The method for production of the composition involves heating a pH-adjusted fermentation broth of microorganisms to a temperature of at least about 100 DEG C for at least about 15 minutes.  Preferably, the microorganism is Gibberella fujikuroi, Streptomyces erythraeus, Bacillus sphaericus, Bacillus thuringiensis or Fusarium moniliforme.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2000US-60196257 2000-04-11
主权利要求
(WO200176612) 1. Claims We claim: L A method for improving biopesticidal activity of materials produced by fermentation comprising the steps of: fermenting a bacterium or fungus to obtain a fermentation broth; adjusting the pH of said fermentation broth to a pH of below about 2.5 with a biologically acceptable acid, or to a pH of above about 8 with a biologically acceptable alkali or base; and heat treating said fermentation broth to a temperature of at least about 100°C for at least about 15 minutes, then cooling to ambient temperature to obtain a pH- adjusted, heat treated composition having improved biopesticidal activity; with the proviso that said fungus is not Myrothecium verrucaria when the pH of said fermentation broth is adjusted to a pH of below about 2.5. 2. The method of claim 1 wherein said fungus or bacterium is selected from the group consisting of Gibberella fujikuroi, Streptomyces erythraeus, Bacillus sphaericus, Bacillus thuringiensis and Fusarium moniliforme. 3. A composition comprising a heat-treated, pH adjusted fermentation broth of a fungus or bacterium selected from the group consisting of Gibberella fujikuroi, Streptomyces erythraeus, Bacillus sphaericus, Bacillus thuringiensis and Fusarium moniliforme; wherein said broth is pH adjusted either to a pH of below about 2.5 with a biologically acceptable acid, or to a pH of above about 8 with a biologically acceptable alkali or base; and wherein said broth is heated to a temperature of at least about 100°C for at least about 15 minutes, then cooled to ambient temperature. 4. A method for control of nematodes on plants which comprises the step of administering to the locus, soil or seed of plants in need of such treatment, a nematocidally effective amount of a composition formed by heating a pH adjusted fermentation broth from a fungus or bacterium; wherein said broth is adjusted to a pH of below about 2.5 with a biologically acceptable acid, or to a pH of above about 8 with a biologically acceptable alkali or base; and wherein said broth is heated to at least 100°C for at least 15 minutes; with the proviso that said fungus is not Myrothecium verrucaria when the pH of said fermentation broth is adjusted to a pH of below about 2.5. 5. The method of claim 4 wherein said composition is administered at a rate of from about 1 to about 200 pounds per acre. 6. The method of claim 4 wherein said fungus or bacterium is selected from the group consisting of Gibberella fujikuroi, Streptomyces erythraeus, Bacillus sphaericus, Bacillus thuringiensis and Fusarium moniliforme. 7. The method of claim 4 wherein said composition further comprises at least one compound selected from the group consisting of herbicides, antimicrobials, fungicides, insecticides, plant growth regulators and nutrients. 8. A method for producing a nematocidal composition comprising the steps of: a) fermenting a bacterium or fungus to obtain a fermentation broth; b) suspending said broth in an aqueous solution; c) adjusting the pH of said broth in aqueous solution to a pH below about pH 2.5 with a biologically acceptable acid; d) heating the pH-treated broth of step c) to a temperature of at least about 100°C for at least about 15 minutes, then cooling to ambient temperature; and, e) recovering said composition; with the proviso that said fungus is not Myrothecium verrucaria. 9. The method of claim 8 wherein said pH is adjusted below about 2. 10. The method of claim 8 wherein said pH is adjusted below about 1.5. 11. The method of claim 8 further comprising adjusting pH of said composition formed in step d) to a range of about pH 4 to about pH 8. 12. The method of claim 8 further comprising adding at least one compound to said composition of step e), said compound selected from the group consisting of herbicides, antimicrobials, fungicides, insecticides, plant growth regulators and nutrients. 13. The method of claim 8 wherein said fermentation broth of step a) has water- soluble biomass and water-insoluble biomass. 14. The method of claim 13 wherein said water-soluble biomass is separated from said water-insoluble biomass, and said water-insoluble biomass is suspended in aqueous solution in step b). 15. A method for producing a nematocidal composition comprising the steps of: a) fermenting a bacterium or fungus to obtain a fermentation broth; b) suspending said broth in an aqueous solution; c) adjusting the pH of said broth in aqueous solution to a pH above about pH 8 with a biologically acceptable alkali or base; d) heating the pH-treated broth of step c) to a temperature of at least about 100°C for at least about 15 minutes, then cooling to ambient temperature; e) recovering said composition. 16. The method of claim 15 wherein said pH is adjusted above about 9. 17. The method of claim 15 wherein said pH is adjusted above about 10. 18. The method of claim 15 further comprising adjusting pH of said composition formed in step d) to a range of about pH 4 to about pH 8. 19. The method of claim 15 further comprising adding at least one compound to said composition of step e), said compound selected from the group consisting of herbicides, antimicrobials, fungicides, insecticides, plant growth regulators and nutrients. 20. The method of claim 15 wherein said fermentation broth of step a) has water- soluble biomass and water-insoluble biomass. 21. The method of claim 20 wherein said water-soluble biomass is separated from said water-insoluble biomass, and said water-insoluble biomass is suspended in aqueous solution in step b).
法律状态
(WO200176612) LEGAL DETAILS FOR WO200176612  Actual or expected expiration date=2003-10-11    Legal state=DEAD    Status=LAPSED     Event publication date=2001-04-10  Event code=WO/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=WO WOUS0111699  Application date=2001-04-10  Standardized application number=2001WO-US11699     Event publication date=2001-10-18  Event code=WO/A1  Event type=Examination events  Published application with search report  Publication country=WO  Publication number=WO200176612  Publication stage Code=A1  Publication date=2001-10-18  Standardized publication number=WO200176612     Event publication date=2001-10-18  Event code=WO/AK  Event indicator=Pos  Event type=Designated states  Designated states AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW    Event publication date=2001-10-18  Event code=WO/AL  Event indicator=Pos  Event type=Designated states  Designated countries for regional patents GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG    Event publication date=2002-05-23  Event code=WO/DFPE  Event type=Examination events  Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)    Event publication date=2002-06-20  Event code=WO/A8  Event indicator=Pos  Event type=Examination events  Modified first page  Publication country=WO  Publication number=WO200176612  Publication stage Code=A8  Publication date=2002-06-20  Standardized publication number=WO200176612     Event publication date=2002-06-20  Event code=WO/AK  Event indicator=Pos  Event type=Designated states  Designated states AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW    Event publication date=2002-06-20  Event code=WO/AL  Event indicator=Pos  Event type=Designated states  Designated countries for regional patents GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG    Event publication date=2002-06-20  Event code=WO/CFP  Event type=Corrections  Corrected version of a pamphlet front page    Event publication date=2002-06-20  Event code=WO/CR1  Event type=Corrections  Correction of entry in section i Correction of entry in sect. i    Event publication date=2003-10-11  Event code=WO/EETL  Event type=Event indicating Not In Force  PCT Application validity period expired. 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专利类型码
A1A8
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