一种​复合​酶​法​提取​南极​磷虾​虾​青​素​的​方法

源语言标题
(CN107033049) 一种​复合​酶​法​提取​南极​磷虾​虾​青​素​的​方法
公开号/公开日
CN107033049 A 2017-08-11 [CN107033049] / 2017-08-11
申请号/申请日
2017CN-0433579 / 2017-06-09
发明人
ZHAO YONGQIANG;LI LAIHAO;YANG XIANQING;YANG SHAOLING;
申请人
CHINESE ACADEMY OF FISHERY SCIENCES;
主分类号
IPC分类号
C07C-403/24
摘要
(CN107033049) 本​发明​公开​了​种​复合​酶​法​提取​南极​磷虾​虾​青​素​的​方法,包括​如下​步骤:(1)将​冷冻​南极​磷虾​搅碎,得到​湿​虾​鲜​样;(2)按​定​水​料​比加​入​蒸馏水,并​添加​复合​酶​得到​南极​磷虾​复合​酶​溶液;(3)将​所​述​南极​磷虾​复合​酶​溶液​在​定​条件下​进行​酶​解,得到​南极​磷虾​酶​解​液;(4)将​所​述​南极​磷虾​酶​解​液​冰水​浴​冷却​后,进行​低温​离心,得到​离心​沉淀物;(5)将​所​述​离心​沉淀物​按​定​料​液​比​用​有机​溶剂​提取​后​离心,得到​南极​磷虾​虾​青​素​提取​液,经​测定​南极​磷虾​虾​青​素​提取​量​在​62.34±1.17​μ​g/g(湿​重)以上。本​发明​采用​复合​酶​提取​南极​磷虾​虾​青​素​的​方法,相对于​单独​酶​解作​用,大大​提高​了​虾​青​素​提取​量;同时​机械​振荡​提取​时间​短,且​为​常温​提取,对虾​青​素​破坏​作用​小,更好​地​提高​了​提取​效率,为​复合​酶​法​提取​虾​青​素​提供​参考。
地址
代理人
代理机构
;
优先权号
2017CN-0433579 2017-06-09
主权利要求
(CN107033049) 1. One composite enzymatic extraction method of astaxanthin Antarctic krill, characterized by comprising the following steps: The (1) frozen cakes Antarctic krill, shrimp to obtain wet; Distilled (2) water was added, and adding a conjugated enzyme to give the Antarctic krill-conjugated enzyme solution; Multizymes Antarctic krill was (3) enzymatically digest solution, to give the Antarctic krill enzymatic digests; After water bath cooling liquid ice will (4) Antarctic krill enzyme, was then centrifuge, leaving a centrifuged precipitate; The pellet (5) was centrifuged after extraction with an organic solvent, to give the Antarctic krill astaxanthin extract. 2. By enzymatic extraction method of astaxanthin Antarctic krill composite according to claim 1, characterized in: step (2), composite enzyme is Alcalase of the 3:2 ratio of alkaline proteases and papain composite composition. 3. Enzymatic extraction method of astaxanthin Antarctic krill using composite according to claim 1, characterized in: step (2), Antarctic krill-conjugated enzyme solution in the water material ratio (V: m, mL: g) was 4:1 - 5:1, enzyme dosage was 1.5% -2.0%. 4. Extracting the Antarctic krill method of astaxanthin using composite enzymatic according to claim 1, characterized in: step (3), conditions were as: time for zymolysis 2.0-2.5 h, 50 - 55 degrees Celsius temperature was digested. 5. Enzymatic extraction Antarctic krill method of astaxanthin using composite according to claim 1, characterized in: step (4), enzymatic liquid ice water bath 20-25min 20-30min,8000-10000r/min then centrifuge cooling time. 6. Enzymatic extraction method of astaxanthin Antarctic krill using composite according to claim 1, characterized in: step (5), the extracting agent is anhydrous ethanol, extraction conditions were: liquor ratio (m: V, g: mL) 1:4 - 1:5, 5 - 10min oscillation time, oscillation extracting 2 times. 7. Extracting the Antarctic krill method of astaxanthin using composite enzymatic according to claim 1, characterized in: step (5), after centrifugation conditions are extracted 5 - 10min 4000-5000r/min centrifugation.
法律状态
(CN107033049) LEGAL DETAILS FOR CN107033049  Actual or expected expiration date=2037-06-09    Legal state=ALIVE    Status=PENDING     Event publication date=2017-06-09  Event code=CN/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=CN CN201710433579  Application date=2017-06-09  Standardized application number=2017CN-0433579     Event publication date=2017-08-11  Event code=CN/A  Event type=Examination events  Published application  Publication country=CN  Publication number=CN107033049  Publication stage Code=A  Publication date=2017-08-11  Standardized publication number=CN107033049
专利类型码
A
国别省市代码
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