(IN201717038972) Microorganisms with increased photosynthetic capacity 机翻标题: 暂无翻译,请尝试点击翻译按钮。

源语言标题
Microorganisms with increased photosynthetic capacity
公开号/公开日
IN201717038972 A 2017-12-08 [IN201717038972] / 2017-12-08
申请号/申请日
2017IN-17038972 / 2017-11-01
发明人
James ROBERTS;Damian CARRIERI;Mark HEINNICKEL;
申请人
LUMEN BIOSCIENCE;RELIANCE HOLDING;
主分类号
IPC分类号
C07K-014/195C07K-014/405C12N-001/20
摘要
(IN201717038972) Microorganisms with increased photosynthetic capacity are described.  Increased photosynthetic capacity is achieved by down regulating activity of the RpaB pathway.  The microorganisms include Cyanobacteria including genetically modified Cyanobacteria.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2015US-62152506 2015-04-24
主权利要求
(IN201717038972)  Groups used in the appended claims. [01 13] Certain embodiments of this invention are described herein, including the best mode known to the inventors for carrying out the invention.  Of course, variations on these described embodiments will become apparent to those of ordinary skill in the art upon reading the foregoing description.  The inventor expects skilled artisans to employ such variations as appropriate, and the inventors intend for the invention to be practiced otherwise than specifically described herein.  Accordingly, this invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law.  Moreover, any combination of the above-described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context. [01 14] Furthermore, if references have been made to patents, printed publications, Journal articles and other written text throughout this specification (referenced materials herein), each of the referenced materials are individually incorporated herein by reference in their entirety for their referenced teaching. [01 15] In closing, it is to be understood that the embodiments of the invention disclosed herein are illustrative of the principles of the present invention.  Other modifications that may be employed are within the scope of the invention.  Thus, by way of example, but not of limitation, alternative configurations of the present invention may be utilized in accordance with the teachings herein.  Accordingly, the present invention is not limited to that precisely as shown and described. 116. The particulars shown herein are by way of example and for purposes of illustrative discussion of the preferred embodiments of the present invention only and are presented in the cause of providing what is believed to be the most useful and readily understood description of the principles and conceptual aspects of various embodiments of the invention.  In this regard, no attempt is made to show structural details of the invention in more detail than is necessary for the fundamental understanding of the invention, the description taken with the drawings and/or examples making apparent to those skilled in the art how the several forms of the invention may be embodied in practice. [01 17] Definitions and explanations used in the present disclosure are meant and intended to be controlling in any future construction unless clearly and unambiguously modified in the following examples or when application of the meaning renders any construction meaningless or essentially meaningless.  In cases where the construction of the term would render it meaningless or essentially meaningless, the definition should be taken from Webster's Dictionary, 3rd Edition or a dictionary known to those of ordinary skill in the art, such as the Oxford Dictionary of Biochemistry and Molecular Biology (Ed. Anthony Smith, Oxford University Press, Oxford, 2004). CLAIMS: CLAIMS: 1. A genetically-modified photosynthetic microorganism comprising a genetic modification that results in down-regulated RpaB pathway activity in the geneticallymodified photosynthetic microorganism as compared to a photosynthetic microorganism of the same species without the genetic modification. 2. A genetically-modified photosynthetic microorganism of claim 1 wherein the genetic modification comprises an exogenous nucleotide sequence that expresses an RpaB decoy under the control of a promoter. 3. A genetically-modified photosynthetic microorganism of claim 2 wherein the RpaB decoy is an N-terminal fragment of RpaB or SrrA comprising a phosphorreceiver domain but no DNA binding domain or a non-functional DNA binding domain. 4. A genetically-modified photosynthetic microorganism of claim 2 wherein the RpaB decoy is an N-terminal fragment of wild type RpaB including Asp56 or a conservative substitution thereof or an N-terminal fragment of wild type SrrA including Asp64 or a conservative substitution thereof. 5. A genetically-modified photosynthetic microorganism of claim 2 wherein the RpaB decoy is N-RpaB or N-SrrA. 6. A genetically-modified photosynthetic microorganism of claim 2 wherein the RpaB decoy is an N-RpaB variant that maintains Asp56 or a conservative substitution thereof or an N-SrrA variant that maintains Asp64 or a conservative substitution thereof. 7. A genetically-modified photosynthetic microorganism of claim 2 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 23 or SEQ ID NO: 33. 8. A genetically-modified photosynthetic microorganism of claim 2 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 23 and SEQ ID NO: 33. 9. A genetically-modified photosynthetic microorganism of claim 1 wherein the genetic modification comprises an exogenous nucleotide sequence that expresses guide RNA and a Cas9 protein. 10. A genetically-modified photosynthetic microorganism of claim 2 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 4 1. 11. A genetically-modified photosynthetic microorganism of claim 2 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 43. 12. A genetically-modified photosynthetic microorganism of claim 2 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 4 1 and SEQ ID NO: 43. 13. A genetically-modified photosynthetic microorganism of claim 9 wherein the guide RNA comprises SEQ ID NO: 42 or a biologically active fragment thereof. 14. A genetically-modified photosynthetic microorganism of claim 9 wherein the Cas9 protein comprises SEQ ID NO: 44 or a variant thereof comprising a conservative substitution. 15. A genetically-modified photosynthetic microorganism of claim 9 wherein the Cas9 protein comprises a substitution at amino acid positions 10 and/or 841. 16. A genetically-modified photosynthetic microorganism of claim 2 wherein the promoter is an inducible promoter. 17. A genetically-modified photosynthetic microorganism of claim 2 wherein the promoter is endogenous to the genome of the genetically-modified photosynthetic microorganism. 18. A genetically-modified photosynthetic microorganism of claim 1 wherein the genetically-modified photosynthetic microorganism is a Cyanobacteria. 19. A genetically-modified photosynthetic microorganism of claim 1 wherein the genetically-modified photosynthetic microorganism is a Cyanobacteria selected from Synechococcus elongatus, Arthrospira maxima, Arthrospira platensis, and Cyanobacterium aponinum. 20. A genetically-modified photosynthetic microorganism of claim 1 wherein the genetically-modified photosynthetic microorganism has increased photosynthetic capacity as compared to a photosynthetic microorganism of the same species without the modification or as compared to a wild type photosynthetic microorganism of the same species. 1. A genetically-modified photosynthetic microorganism of claim 1 wherein the genetically-modified photosynthetic microorganism shows increased photosynthetic biomass accumulation from photoautotrophic growth as compared to a photosynthetic microorganism of the same species without the modification or as compared to a wild type photosynthetic microorganism of the same species. 22. A method of increasing photosynthetic capacity and/or biomass accumulation of a photosynthetic microorganism comprising modifying the photosynthetic microorganism to reduce RpaB pathway activity within the photosynthetic microorganism as compared to a photosynthetic microorganism of the same species without the modification or as compared to a wild type photosynthetic microorganism of the same species. 23. A method of claim 22 wherein the modifying comprises inserting an exogenous nucleotide sequence that expresses an RpaB decoy under the control of a promoter into the photosynthetic microorganism. 24. A method of claim 23 wherein the RpaB decoy is an N-terminal fragment of RpaB or SrrA comprising a phosphor-receiver domain but no DNA binding domain or a non-functional DNA binding domain. 25. A method of claim 23 wherein the expressed RpaB decoy is an N-terminal fragment of wild type RpaB including Asp56 or a conservative substitution thereof an N-terminal fragment of wild type SrrA including Asp64 or a conservative substitution thereof. 26. A method of claim 23 wherein the expressed RpaB decoy is N-RpaB or N-SrrA. 27. A method of claim 23 wherein the expressed RpaB decoy is an N-RpaB variant that maintains Asp56 or a conservative substitution thereof or an N-SrrA variant that maintains Asp64 or a conservative substitution thereof. 28. A method of claim 23 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 23 or SEQ ID NO: 33. 29. A method of claim 23 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 23 and SEQ ID NO: 33. 30. A method of claim 23 wherein the exogenous nucleotide sequence expresses guide RNA and a Cas9 protein. 3. 1. A method of claim 23 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 4 1. 32. A method of claim 23 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 43. 33. A method of claim 23 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 4 1 and SEQ ID NO: 43. 34. A method of claim 30 wherein the guide RNA comprises SEQ ID NO: 42 or a biologically active fragment thereof. 35. A method of claim 30 wherein the Cas9 protein comprises SEQ ID NO: 44 or a variant thereof comprising a conservative substitution. 36. A method of claim 30 wherein the Cas9 protein comprises a substitution at amino acid positions 10 and/or 841. 37. A method of claim 23 wherein the promoter is an inducible promoter. 38. A method of claim 23 wherein the promoter is endogenous to the genome of the genetically-modified photosynthetic microorganism. 39. A method of claim 22 wherein the genetically-modified photosynthetic microorganism is a Cyanobacteria. 40. A method of claim 22 wherein the genetically-modified photosynthetic microorganism is a Cyanobacteria selected from Synechococcus elongatus, Arthrospira maxima, Arthrospira platensis, and Cyanobacterium aponinum. 1. A method of claim 22 wherein the genetically-modified photosynthetic microorganism has increased photosynthetic capacity as compared to a photosynthetic microorganism of the same species without the modification or as compared to a wild type photosynthetic microorganism of the same species. 42. A method of claim 22 wherein the genetically-modified photosynthetic microorganism shows increased photosynthetic biomass accumulation from photoautotrophic growth as compared to a photosynthetic microorganism of the same species without the modification or as compared to a wild type photosynthetic microorganism of the same species. 43. A method of increasing photoautotrophic growth of a photosynthetic microorganism comprising: Modifying the photosynthetic microorganism to reduce RpaB pathway activity within the photosynthetic microorganism as compared to a photosynthetic microorganism of the same species without the modification or as compared to a wild type photosynthetic microorganism of the same species and culturing the modified photosynthetic microorganism in a liquid culture thereby increasing photoautotrophic growth of the photosynthetic microorganism as compared to the photosynthetic microorganism of the same species without the modification or as compared to the wild type photosynthetic microorganism of the same species. 44. A method of claim 43 wherein the modifying comprises inserting an exogenous nucleotide sequence that expresses an RpaB decoy under the control of a promoter into the photosynthetic microorganism. 45. A method of claim 44 wherein the RpaB decoy is an N-terminal fragment of RpaB or SrrA comprising a phosphor-receiver domain but no DNA binding domain or a non-functional DNA binding domain. 46. A method of claim 44 wherein the expressed RpaB decoy is an N-terminal fragment of wild type RpaB including Asp56 or a conservative substitution thereof an N-terminal fragment of wild type SrrA including Asp64 or a conservative substitution thereof. 47. A method of claim 44 wherein the expressed RpaB decoy is N-RpaB or N-SrrA. 48. A method of claim 44 wherein the expressed RpaB decoy is an N-RpaB variant that maintains Asp56 or a conservative substitution thereof or an N-SrrA variant that maintains Asp64 or a conservative substitution thereof. 49. A method of claim 44 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 23 or SEQ ID NO: 33. 50. A method of claim 44 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 23 and SEQ ID NO: 33. 1. A method of claim 44 wherein the exogenous nucleotide sequence that expresses guide RNA and a Cas9 protein. 52. A method of claim 44 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 4 1. 53. A method of claim 44 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 43. 54. A method of claim 44 wherein the exogenous nucleotide sequence comprises SEQ ID NO: 4 1 and SEQ ID NO: 43. 55. A method of claim 5 1 wherein the guide RNA comprises SEQ ID NO: 42 or a biologically active fragment thereof. 56. A method of claim 5 1 wherein the Cas9 protein comprises SEQ ID NO: 44 or a variant thereof comprising a conservative substitution. 57. A method of claim 5 1 wherein the Cas9 protein comprises a substitution at amino acid positions 10 and/or 841. 58. A method of claim 44 wherein the promoter is an inducible promoter. 59. A method of claim 44 wherein the promoter is endogenous to the genome of the genetically-modified photosynthetic microorganism. 60. A method of claim 43 wherein the modified photosynthetic microorganism is a Cyanobacteria. 6. 1. A method of claim 43 wherein the modified photosynthetic microorganism is a Cyanobacteria selected from Synechococcus elongatus, Arthrospira maxima, Arthrospira piatensis, and Cyanobacterium aponinum.
法律状态
(IN201717038972) LEGAL DETAILS FOR IN201717038972  Actual or expected expiration date=2037-11-01    Legal state=ALIVE    Status=PENDING     Event publication date=2017-11-01  Event code=IN/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=IN IN201717038972  Application date=2017-11-01  Standardized application number=2017IN-17038972     Event publication date=2017-12-08  Event code=IN/A  Event indicator=Pos  Event type=Examination events  Application laid open  Publication country=IN  Publication number=IN201717038972  Publication stage Code=A  Publication date=2017-12-08  Standardized publication number=IN201717038972
专利类型码
A
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