A novel fluorescent recombinant cell-based biosensor for screening NLRP3 inflammasome inhibitors 机翻标题: 暂无翻译,请尝试点击翻译按钮。

2020-02-10

Nucleotide-binding oligomerization domain-like receptor pyrin-domain-containing protein 3 (NLRP3) inflammasomes trigger a defense mechanism in response to physiological or pathological reactions caused by various injuries, and they have critical roles in the development of many major diseases. The development of plant source anti-inflammatory agents and the assessment of their anti-inflammatory properties still face many challenges, such as high costs and long testing periods. Exogenous anti-inflammatory factors can be evaluated at the protein level using the NLRP3-green fluorescent protein (GFP) THP-1 cell-based biosensor. To achieve this, THP-1 cells were stably transfected with a plasmid encoding the GFP reporter gene that is under the transcriptional regulation of the NLRP3 promoter. Because the NLRP3 promoter controls the green fluorescent signal, the fluorescence is an indicator of NLRP3 activity. Our results show the fluorescence intensity was dependent on both the degree of inflammation and the effect of the anti-inflammatory agent. Here, we successfully used the biosensor to screen an NLRP3 inhibitor in the presence of polyphenols. Our results demonstrate the potential of this cell-based biosensor in monitoring the development of inflammation and in screening inflammatory inhibitors.

暂无翻译结果,您可以尝试点击头部的翻译按钮。

点击如下链接查看来源站点的原文: 原文链接

最新评论

暂无评论。

登录后可以发表评论


意见反馈
返回顶部
调查问卷