(EP2650376) Method for producing an l-amino acid by reducing the intracellular hydrogen peroxide concentration 机翻标题: 暂无翻译,请尝试点击翻译按钮。

源语言标题
(EP2650376) Method for producing an l-amino acid by reducing the intracellular hydrogen peroxide concentration
公开号/公开日
EP2650376 EP2650376 EP2650376 / 2020-03-04 2016-07-20 2013-10-16
申请号/申请日
EP11846714 / 2011-12-08
发明人
DOI HIDETAKAHOSHINO YASUSHIMASUMITSU YURIUSUDA YOSHIHIRO;
申请人
AJINOMOTO;
主分类号
IPC分类号
C07K-014/245 C12N-001/20 C12N-001/38 C12N-015/09 C12P-013/08
摘要
(EP2650376) In a method for producing an L-amino acid comprising culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium, a bacterium which has been subjected to a modification selected from the group consisting of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.
机翻摘要
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地址
代理人
(EP2650376) Strehl Schübel-Hopf & Partner ([DE]) Reg. Nb: 100060622
代理机构
;
优先权号
2010JP-0276062 2011WO-JP78380
主权利要求
(EP2650376) 1. A method for producing an L-amino acid comprising culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium, wherein the intracellular hydrogen peroxide concentration of the bacterium is reduced by:   subjecting the bacterium to a modification selected from the group consisting of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof; and/or   adding thiourea to the medium. 2. The method according to any one of claim 1, wherein the bacterium belongs to the genus Escherichia, Pantoea, or Enterobacter. 3. The method according to claim 2, wherein the bacterium is Escherichia coli, Pantoea ananatis, or Enterobacter aerogenes. 4. The method according to any one of claims 1 to 3, wherein the oxyS gene encodes RNA having the nucleotide sequence of SEQ ID NO: 9, or a conservative variant thereof. 5. The method according to any one of claims 1 to 4, wherein the fixABC genes encode proteins having the amino acid sequences of SEQ ID NOS: 11, 13, and 15, or a conservative variant thereof. 6. The method according to any one of claims 1 to 5, wherein the carbon source is a fatty acid. 7. The method according to claim 6, wherein the fatty acid is oleic acid. 8. The method according to claim 6, wherein the fatty acid is a mixture of fatty acids derived from a fat or oil. 9. The method according to any one of claims 1 to 5, wherein the carbon source is an alcohol. 10. The method according to claim 9, wherein the alcohol is glycerol. 11. The method according to claim 9, wherein the alcohol is ethanol. 12. The method according to any one of claims 1 to 5, wherein the carbon source is a mixture of a fatty acid and glycerol obtained by hydrolyzing a fat or oil. 13. The method according to claim 11, wherein the bacterium is Escherichia coli, and has been modified so that it can aerobically utilize ethanol. 14. The method according to any one of claims 1 to 13, wherein the L-amino acid is L-lysine. 15. The method according to claim 14, wherein activity or activities of one or more kinds of enzymes selected from the group consisting of dihydrodipicolinate reductase, diaminopimelate decarboxylase, diaminopimelate dehydrogenase, phosphoenolpyruvate carboxylase, aspartate aminotransferase, diaminopimelate epimerase, aspartate semialdehyde dehydrogenase, tetrahydrodipicolinate succinylase, and succinyldiaminopimelate deacylase are enhanced, and/or activity of lysine decarboxylase is attenuated.
法律状态
GRANTED
专利类型码
B1 A4 A1
国别省市代码
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