Method for obtaning thin fibril collagen by contacting native collagen with an antibody 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2010255457 A1 2010-10-07 [US20100255457]US9458224 B2 2016-10-04 [US9458224] / 2010-10-072016-10-04
申请号/申请日
2009US-12419689 / 2009-04-07
发明人
ORGEL JOSEPH;ANTIPOVA OLGA;
申请人
MATRIX ODYSSEY;
主分类号
IPC分类号
A01N-001/02C07K-014/78
摘要
(US9458224) A collagen material having a form of thin fibrils generally free of fibril-bundling proteogylcan interactions, and a method for providing the thin fibril collagen material from native collagen fibers.  The method uses proteoglycan antibodies to disassociate the proteoglycan interactions in bundled collagen fibrils to provide the constituent fibrils.  The process can be used as a model for arthritis and the resulting fibrils can be used to form new extracellular matrix biomaterials and new tissues.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2009US-12419689 2009-04-07
主权利要求
(US9458224) What is claimed is: 1.  A method of obtaining fibril collagen, comprising: providing isolated collagen fibers, wherein the collagen fibers comprise native type II collagen fibers; and   treating the collagen fibers with an antibody to obtain collagen fibrils, wherein the antibody comprises an anti-biglycan antibody. 2. The method according to claim 1, wherein each of the collagen fibers are formed of constituent fibrils and treating the collagen fibers with an antibody comprises dividing the collagen fibers into the constituent fibrils. 3. The method according to claim 1, wherein treating the collagen fibers with an antibody comprises reducing a size of the collagen fibers. 4. The method according to claim 1, wherein treating the collagen fibers with an antibody comprises reducing or removing an amount of biglycan from the collagen fibers. 5. The method according to claim 1, wherein treating the collagen fibers with an antibody comprises incubating the collagen fibers with the antibody. 6. The method according to claim 1, further comprising incubating the collagen fibers with the antibody in a buffered solution. 7. The method according to claim 1, wherein providing collagen fibers comprises homogenizing a tissue containing the collagen fibers. 8. The method according to claim 1, wherein providing collagen fibers comprises removing the collagen fibers from a notochord. 9. The method according to claim 1, wherein providing collagen fibers comprises denaturing a tissue containing the collagen fibers. 10. The method according to claim 1, further comprising forming fibril bundles including the collagen fibrils. 11. The method according to claim 10, further comprising adding biglycan, decorin, fibronectin, aggrecan, polyethylene glycol, or combinations thereof to the collagen fibrils to form the fibril bundles. 12. The method according to claim 1, wherein each of the collagen fibrils has a width of 11 to 17 nanometers. 13. The method according to claim 12, wherein the collagen fibrils have a width of 11.5 to 12.5 nanometers. 14. The method according to claim 12, wherein the collagen fibrils comprise constituent fibrils of the native type II collagen fiber. 15. The method according to claim 12, wherein the collagen fibrils are substantially free of proteoglycan. 16. The method according to claim 12, wherein the collagen fibrils are stored in a buffered solution having a pH of 7.0 to 7.5. 17. The method according to claim 8, further comprising forming a fibril bundle matrix including the collagen fibrils and one or more of biglycan, decorin, fibronectin, aggrecan, and polyethylene glycol.
法律状态
(US9458224) LEGAL DETAILS FOR US2010255457  Actual or expected expiration date=2034-11-19    Legal state=ALIVE    Status=GRANTED     Event publication date=2009-04-07  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US12419689  Application date=2009-04-07  Standardized application number=2009US-12419689     Event publication date=2009-04-07  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: STEADMAN, DAVID J    Event publication date=2009-04-07  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1656     Event publication date=2009-04-07  Event code=US/SMALL  Event type=Administrative notifications  Appl Has Filed a Verified Statement of Micro to Small Entity Status Business Entity Status: MICRO    Event publication date=2009-04-07  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=2009-04-07  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: 3722649.00004    Event publication date=2009-04-07  Event code=US/CUST  Event type=Examination events  Attorney/Agent Customer Number Customer Nbr: 24573    Event publication date=2009-04-07  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: ILLINOIS INSTITUTE OF TECHNOLOGY, ILLINOIS  Effective date of the event=2009-04-03  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:ORGEL, JOSEPH ANTIPOVA, OLGA REEL/FRAME:022516/0567     Event publication date=2009-04-27  Event code=US/APPFILEREC  Event type=Administrative notifications  Event type=OAO  Filing Receipt    Event publication date=2009-10-07  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2010-01-07  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=2010-10-07  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2010255457  Publication stage Code=A1  Publication date=2010-10-07  Standardized publication number=US20100255457     Event publication date=2010-10-07  Event code=US/PGPUBN  Event indicator=Pos  Event type=Examination events  Event type=OAO  PG-Pub Issue Notification    Event publication date=2011-01-31  Event code=US/ELC  Event type=Examination events  Event type=OAI  Response to Election / Restriction Filed    Event publication date=2011-05-22  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=2011-09-09  Event code=US/RESTREQ  Event type=Examination events  Event type=Corrections  Event type=OAO  Restriction/Election Requirement    Event publication date=2011-11-16  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2012-03-08  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=2012-03-08  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=2012-04-23  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2012-08-27  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=2012-08-27  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=2012-10-10  Event code=US/CTFR  Event type=Examination events  Event type=OA  Event type=OAO  Final Rejection    Event publication date=2013-01-15  Event code=US/NOAPLF  Event type=APL  Notice of Appeal Filed    Event publication date=2013-04-18  Event code=US/APB  Event type=APL  Event type=OAI  Appeal Brief Filed    Event publication date=2013-04-18  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=2013-04-23  Event code=US/APLRVC  Event type=APL  Appeal Brief Review Complete    Event publication date=2013-06-20  Event code=US/APEA  Event type=APL  Event type=OAO  Examiner's Answer to Appeal Brief    Event publication date=2016-03-21  Event code=US/BPAIR  Event type=APL  BPAI Decision - Examiner Reversed    Event publication date=2016-06-03  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=2016-07-11  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: MATRIX ODYSSEY, LLC, ILLINOIS  Effective date of the event=2016-04-27  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNOR:ILLINOIS INSTITUTE OF TECHNOLOGY REEL/FRAME:039125/0731     Event publication date=2016-07-19  Event code=US/POAC  Event type=Change of name or address  Change in Power of Attorney (May Include Associate POA)    Event publication date=2016-08-26  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: MATRIX ODYSSEY, LLC, ILLINOIS  Effective date of the event=2016-04-27  CORRECTIVE ASSIGNMENT TO CORRECT THE ASSIGNEE ADDRESS PREVIOUSLY RECORDED ON REEL 039125 FRAME 0731. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT ASSIGNOR:ILLINOIS INSTITUTE OF TECHNOLOGY REEL/FRAME:039843/0282     Event publication date=2016-08-26  Event code=US/SMALL  Event type=Administrative notifications  Appl Has Filed a Verified Statement of Micro to Small Entity Status    Event publication date=2016-09-01  Event code=US/APRDY  Event indicator=Pos  Event type=Examination events  Application Is Considered Ready for Issue    Event publication date=2016-09-14  Event code=US/PAT  Event indicator=Pos  Event type=Event indicating In Force  Patented Case    Event publication date=2016-09-14  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Event type=Restitution or restoration  Issue Notification Mailed    Event publication date=2016-10-04  Event code=US/B2  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as second publication  Publication country=US  Publication number=US9458224  Publication stage Code=B2  Publication date=2016-10-04  Standardized publication number=US9458224     Event publication date=2016-10-04  Event code=US/354  Event indicator=Pos  Event type=Extension of term of duration of protection  Patent term extended under  35 U.S.C 154(b) until/for Delays (A,B,C): 724  Overlap Delays: 0  Non Overlap Delays: 2104   PTO Office Delays: 0  Applicant Delays: 52  Adjustment total:  Number of days of extension=2052    Event publication date=2017-05-31  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: NATIONAL SCIENCE FOUNDATION, VIRGINIA  Effective date of the event=2017-02-14  CONFIRMATORY LICENSE ASSIGNOR:ILLINOIS INSTITUTE OF TECHNOLOGY REEL/FRAME:042625/0953     Event publication date=2017-09-26  Event code=US/CC  Event type=Corrections  Event type=Restitution or restoration  Certificate of Correction - Post Issue Communication
专利类型码
A1B2
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