Method for producing lower alkyl ester 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2016265019 A1 2016-09-15 [US20160265019]US9708637 B2 2017-07-18 [US9708637] / 2016-09-152017-07-18
申请号/申请日
2016US-15164381 / 2016-05-25
发明人
SHAKULOV RUSTEM SAIDOVICH;KLYACHKO ELENA VITALIEVNA;DOROSHENKO VERA GEORGIEVNA;AIRIKH LARISA GOTLIBOVNA;
申请人
AJINOMOTO;
主分类号
IPC分类号
C12N-009/02C12N-009/90C12N-009/92C12P-013/20C12P-013/22C12P-013/24C12P-021/02
摘要
(US9708637) A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2007RU-0135818 2007-09-27 2008US-12238704 2008-09-26 2016US-15164381 2016-05-25
主权利要求
(US9708637) 1. A method for producing a lower alkyl ester of alpha -L-aspartyl-L-phenylalanine, comprising: A) cultivating a bacterium of Escherichia coli in a culture medium which is able to produce and accumulate L-phenylalanine in the medium, and B) synthesizing the lower alkyl ester of alpha -L-aspartyl-L-phenylalanine from aspartic acid or a derivative thereof and the L-phenylalanine obtained in step A); wherein the bacterium comprises a DNA comprising: i) a gene selected from the group consisting of tyrA, pheA, pgi, ilvE, ilvA, tdcB, sdaA, sdaB, argA, argG, proB, thrB, and combinations thereof, and ii) a DNA fragment able to be transcribed and encoding the peptide of SEQ ID NO: 2, or a variant thereof consisting of a deletion, insertion, substitution, or addition of 1 amino acid as compared to SEQ ID NO: 2; and wherein said DNA fragment of ii) is attached to the 3' end of said gene of i) and consequently enhances production of an L-amino acid.
法律状态
(US9708637) LEGAL DETAILS FOR US2016265019  Actual or expected expiration date=2028-09-26    Legal state=ALIVE    Status=GRANTED     Event publication date=2016-05-25  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US15164381  Application date=2016-05-25  Standardized application number=2016US-15164381     Event publication date=2016-05-25  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: ROBINSON, HOPE A    Event publication date=2016-05-25  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1652     Event publication date=2016-05-25  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: US-347D Customer Nbr: 38108    Event publication date=2016-05-25  Event code=US/ENT  Event type=Administrative notifications  Business Entity Status: UNDISCOUNTED    Event publication date=2016-05-25  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=2016-05-25  Event code=US/PTARDY  Event indicator=Pos  Event type=Examination events  Patent Term Adjustment - Ready for Examination    Event publication date=2016-05-25  Event code=US/ENTYSTA  Event type=Administrative notifications  Entity Status Set to Undiscounted    Event publication date=2016-05-26  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2016-06-09  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner in GAU    Event publication date=2016-06-10  Event code=US/APPFILEREC  Event type=Administrative notifications  Event type=OAO  Filing Receipt    Event publication date=2016-06-29  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: AJINOMOTO CO., INC., JAPAN  Effective date of the event=2016-06-10  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:SHAKULOV, RUSTEM SAIDOVICH KLYACHKO, ELENA VITALIEVNA DOROSHENKO, VERA GEORGIEVNA AND OTHERS REEL/FRAME:039039/0570     Event publication date=2016-09-15  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2016265019  Publication stage Code=A1  Publication date=2016-09-15  Standardized publication number=US20160265019     Event publication date=2016-09-15  Event code=US/PGPUBN  Event indicator=Pos  Event type=Examination events  Event type=OAO  PG-Pub Issue Notification    Event publication date=2016-10-17  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2017-03-30  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2017-05-05  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=2017-05-31  Event code=US/RECEIPTCOR  Event type=Administrative notifications  Event type=OAO  Filing Receipt - Corrected    Event publication date=2017-06-06  Event code=US/APRDY  Event indicator=Pos  Event type=Examination events  Application Is Considered Ready for Issue    Event publication date=2017-06-28  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Issue Notification Mailed    Event publication date=2017-07-18  Event code=US/B2  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as second publication  Publication country=US  Publication number=US9708637  Publication stage Code=B2  Publication date=2017-07-18  Standardized publication number=US9708637     Event publication date=2017-07-18  Event code=US/354  Event indicator=Pos  Event type=Extension of term of duration of protection  Patent term extended under  35 U.S.C 154(b) until/for Delays (A,B,C): 0  Overlap Delays: 0  Non Overlap Delays: 0   PTO Office Delays: 0  Applicant Delays: 80  Adjustment total:  Number of days of extension=0
专利类型码
A1B2
国别省市代码
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