Purification and characterization of thermostable superoxide dismutase from Anoxybacillus gonensis KA 55 MTCC 12684 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
International Journal of Biological Macromolecules: Structure, Function and Interactions
年/卷/期
2018 / 117 /
页码
1133-1139
ISSN号
0141-8130
作者单位
Shoolini Univ, Fac Appl Sci & Biotechnol, Solan 173229, India;Indian Vet Res Inst, Reg Stn, Palampur 176061, Himachal Prades, India;Indian Vet Res Inst, Reg Stn, Palampur 176061, Himachal Prades, India;Shoolini Univ, Fac Appl Sci & Biotechnol, Solan 173229, India;Shoolini Univ, Fac Appl Sci & Biotechnol, Solan 173229, India;Shoolini Univ, Fac Appl Sci & Biotechnol, Solan 173229, India;
作者
Bhatia, Kavita;Mal, Gorakh;Bhar, Rasbihari;Jyoti;Attri, Chandrika;Seth, Amit;
摘要
A thermostable superoxide dismutase from thermophilic bacterium Anoxybacillus gonensis KA 55 MTCC 12684 which was isolated from Manikaran hotspring of Himachal Pradesh was purified to apparent homogeneity by fractional ammonium sulphate precipitation and anion exchange chromatography. A purification factor of 33.1-fold was achieved, with the purified enzyme exhibiting specific activity of 5758.4 U/mg protein. The purified superoxide dismutase was optimally active at pH 9.0 and displayed stability over a broad pH range of 7.0-10.0 and was stable up to 70 degrees C. SOD was localized in polyacrylamide gel by activity staining, based on the reduction of nitroblue tetrazolium (NET) by superoxide ion. The molecular weight of superoxide dismutase was calculated as 31 kDa by SDS-PAGE. The K-m and V-max values of purified enzyme were found to be 1.002 mM and 14,285.71 U/mg of protein respectively. Tests of inhibitors indicated that the enzyme activity was inhibited by hydrogen peroxide and potassium cyanide but not by sodium azide showing that purified SOD was Cu/ZnSod. (C) 2018 Elsevier B.V. All rights reserved.
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关键词/主题词
Anoxybacillus gonensis;Superoxide dismutase;Purification and Cu/ZnSOD;
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