Method for producing L-amino acid 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2015275246 A1 2015-10-01 [US20150275246]US9487806 B2 2016-11-08 [US9487806] / 2015-10-012016-11-08
申请号/申请日
2015US-14735303 / 2015-06-10
发明人
TOYAZAKI MIKU;NOGUCHI KEIKO;MORIYA MIKA;UEHARA YURI;
申请人
AJINOMOTO;
主分类号
IPC分类号
C12N-015/70C12P-013/04C12P-013/08
摘要
(US9487806) A method for producing of an L-amino acid is provided.  An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, which has been modified so that the acpP-fabF operon is attenuated, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2013JP-0218221 2013-10-21 2014WO-JP77993 2014-10-21
主权利要求
(US9487806) 1. A method for producing an L-aminoacid comprising: culturing an Escherichia coli bacterium having an L-aminoacid-producing ability in a medium so that an L-aminoacid is produced and accumulates in the medium or the cells of the Escherichia coli bacterium; and collecting the L-aminoacid from the medium or the cells, wherein the Escherichia coli bacterium has been genetically modified to have attenuated but not eliminated expression of the chromosomal acyl carrier protein (acpP) gene as compared to a non-modified Escherichia coli bacterium. 7. A method for producing L-lysine comprising: culturing an Escherichia coli bacterium having L-lysine-producing ability in a medium so that L-lysine is produced and accumulates in the medium or the cells of the Escherichia coli bacterium; and collecting the L-lysine from the medium or the cells, wherein the Escherichia coli bacterium has been genetically modified to have attenuated but not eliminated expression of the chromosomal acpP gene as compared with a non-modified Escherichia coli bacterium by modifying an expression control sequence of the chromosomal acpP gene. 8. A method for producing L-lysine comprising: culturing an Escherichia coli having L-lysine-producing ability in a medium so that L-lysine is produced and accumulates in the medium or the cells of the Escherichia coli bacterium; and collecting the L-lysine from the medium or the cells, wherein the Escherichia coli bacterium has been genetically modified to have attenuated but not eliminated expression of the chromosomal acpP gene as compared with a non-modified Escherichia coli bacterium, wherein the expression of the chromosomal acpP gene is attenuated by replacing the cytosine of the chromosomal acpP gene at the nucleotide corresponding to position 177 of the nucleotide sequence of SEQ ID NO: 7 with another base. 9. A method for producing L-lysine comprising: culturing an Escherichia coli having L-lysine-producing ability in a medium so that L-lysine is produced and accumulates in the medium or the cells of the Escherichia coli bacterium; and collecting the L-lysine from the medium or the cells, wherein the Escherichia coli bacterium has been genetically modified to have attenuated but not eliminated expression of the chromosomal acpP gene as compared with a non-modified Escherichia coli bacterium, wherein the expression of the chromosomal acpP gene is attenuated by replacing the cytosine of the chromosomal acpP gene at the nucleotide corresponding to position 177 of the nucleotide sequence of SEQ ID NO: 7 with adenine.
法律状态
(US9487806) LEGAL DETAILS FOR US2015275246  Actual or expected expiration date=2034-10-21    Legal state=ALIVE    Status=GRANTED     Event publication date=2015-06-10  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US14735303  Application date=2015-06-10  Standardized application number=2015US-14735303     Event publication date=2015-06-10  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: STEADMAN, DAVID J    Event publication date=2015-06-10  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1656     Event publication date=2015-06-10  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: US-525 Customer Nbr: 38108    Event publication date=2015-06-10  Event code=US/ENT  Event type=Administrative notifications  Business Entity Status: UNDISCOUNTED    Event publication date=2015-06-10  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=Yes     Event publication date=2015-06-10  Event code=US/PTARDY  Event indicator=Pos  Event type=Examination events  Patent Term Adjustment - Ready for Examination    Event publication date=2015-06-10  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2015-06-10  Event code=US/ENTYSTA  Event type=Administrative notifications  Entity Status Set to Undiscounted    Event publication date=2015-06-22  Event code=US/APPFILEREC  Event type=Administrative notifications  Event type=OAO  Filing Receipt    Event publication date=2015-07-22  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner in GAU    Event publication date=2015-07-29  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: AJINOMOTO CO., INC., JAPAN  Effective date of the event=2015-07-17  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:TOYAZAKI, MIKU NOGUCHI, KEIKO MORIYA, MIKA AND OTHERS REEL/FRAME:036205/0651     Event publication date=2015-10-01  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2015275246  Publication stage Code=A1  Publication date=2015-10-01  Standardized publication number=US20150275246     Event publication date=2015-10-01  Event code=US/PGPUBN  Event indicator=Pos  Event type=Examination events  Event type=OAO  PG-Pub Issue Notification    Event publication date=2016-02-18  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2016-04-26  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2016-06-14  Event code=US/EXAC  Event type=Examination events  Event type=OAO  Examiner's Amendment Communication    Event publication date=2016-06-29  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=2016-07-08  Event code=US/ANA  Event type=Corrections  Event type=Examination events  Event type=OA  Amendment after Notice of Allowance (Rule 312)    Event publication date=2016-07-13  Event code=US/ANA  Event type=Corrections  Event type=Examination events  Event type=OA  Amendment after Notice of Allowance (Rule 312)    Event publication date=2016-07-13  Event code=US/N271  Event type=Examination events  Event type=OAO  Response to Amendment under Rule 312    Event publication date=2016-07-15  Event code=US/N271  Event type=Examination events  Event type=OAO  Response to Amendment under Rule 312    Event publication date=2016-07-16  Event code=US/ANA  Event type=Corrections  Event type=Examination events  Event type=OA  Amendment after Notice of Allowance (Rule 312)    Event publication date=2016-08-18  Event code=US/N271  Event type=Examination events  Event type=OAO  Response to Amendment under Rule 312    Event publication date=2016-09-28  Event code=US/APRDY  Event indicator=Pos  Event type=Examination events  Application Is Considered Ready for Issue    Event publication date=2016-10-19  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Issue Notification Mailed    Event publication date=2016-11-08  Event code=US/B2  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as second publication  Publication country=US  Publication number=US9487806  Publication stage Code=B2  Publication date=2016-11-08  Standardized publication number=US9487806     Event publication date=2016-11-08  Event code=US/354  Event indicator=Pos  Event type=Extension of term of duration of protection  Patent term extended under  35 U.S.C 154(b) until/for Delays (A,B,C): 0  Overlap Delays: 0  Non Overlap Delays: 0   PTO Office Delays: 0  Applicant Delays: 14  Adjustment total:  Number of days of extension=0
专利类型码
A1B2
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