(CN106434505) 本发明公开了种利用生物柴油副产物甘油合成D‑乳酸的重组菌及其应用,属于基因工程领域。本发明提供的重组菌,是将D‑乳酸脱氢酶基因ldhA导入敲除了1,3‑丙二醇氧化还原酶基因dhaT和醛还原酶/醇脱氢酶基因yqhD的宿主重组肺炎克雷伯氏菌中得到的工程菌株。本发明实现了以生物柴油副产物甘油为底物合成D‑乳酸,在摇瓶水平,产量和转化率分别达到12.7g/L和50.4%,光学纯度为100%,具有光学纯度和转化率高、生产成本低的特点。
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