Process for extracting lipids for use in production of biofuels 机翻标题: 暂无翻译,请尝试点击翻译按钮。

源语言标题
(KR20160101990) 바이오연료의 생산에 사용하기 위한 지질 추출 방법
公开号/公开日
KR20160101990 A 2016-08-26 [KR20160101990] / 2016-08-26
申请号/申请日
2016KR-7019377 / 2014-12-18
发明人
APT KIRK;BARCLAY WILLIAM;BLAZER MICAH;BORDEN JACOB;BURJA ADAM;DONG DANIEL;DURAZO ARMANDO;DUMENIL JEAN CHARLES;EDGE ARTHUR;HANSEN JON;HOFLER ALEXANDRA;JEFFERS DAVID;LYON CHRIS;PAI VIDYA;PFEIFER JOSEPH W III;SELLERS MARTIN J;
申请人
DSM;
主分类号
IPC分类号
C11B-001/02C11B-001/10C11B-001/12C12N-001/06C12N-009/24C12N-009/26C12P-007/64
摘要
(KR20160101990) Methods and systems used to extract lipids suitable in production of biofuels from a fermentation broth may include using heat to pre-treat the fermentation broth in order to more easily extract a product from oleaginous microorganisms in the broth.  Additionally or alternatively, a combination of enzymes including amylase, 1-4 mannosidase, and 1-3 mannosidase may be used to break down cell walls of the oleaginous microorganisms.  Residual broth water may be recycled and used as imbibition water for washing a process feedstock to extract sugar.  (From US2016355749 A1)
机翻摘要
暂无翻译结果,您可以尝试点击头部的翻译按钮。
地址
代理人
代理机构
;
优先权号
2013US-61918850 2013-12-20 2014WO-US71055 2014-12-18
主权利要求
(KR20160101990)  1. Yu Ji-seong (oleaginous) whole taking effect Boulos where the microbe contains (broth) about 90.deg.C to about 150.deg.C or about 100.deg.C to about 150.deg.C or about 110.deg.C to about 150.deg.C or about 120.deg.C to about 150.deg.C or about 130.deg.C to heats at about 150.deg.C temperature with the low of preprocessing phase.  And Succeeding from the Yu Ji-seong microbe the product the low of extracting phase As for under including, from whole taking effect Boulos the suitable geological features extraction method which produces Bio fuel. 2. There is in a 1st claim, Whole taking effect Boulos about 30 minutes to about 18 hours, or 3 timeout to about 18 hours, or 3 timeout to as for under heating for about 8 hours phase as for under including method. 3. Is in the 1st claim or the second claim, From 45.deg.C to 80.deg.C heats whole taking effect Boulos where the Yu Ji-seong microbe contains at 60 minute under with, the time when whole taking effect Boulos where the Yu Ji-seong microbe contains stays from 45.deg.C to 80.deg.C as for under minimizing phase the low of including method. 4. The 1st claim to there is in which one claim in the 3rd claim, Whole taking effect Boulos 1 per minute about 0.1 to at about 80.deg.C average speed as for under heating phase as for under including method. 5. Is in the 1st claim to which one claim in the fourth claim, Adds the mountain or a salty taste with the phase which regulates the ph of whole taking effect Boulos in addition the low of including method. 6. Is in the 1st claim to which one claim in the fifth claim, In order to permit additional isothermal processing (isothermal processing) whole taking effect Boulos at temperature of about 60.deg.C excess or about 70.deg.C excess or about 80.deg.C excess or about 85.deg.C excess or about 90.deg.C excess at the time of cooling as for height phase in addition as for under including method. 7. There is in a 6th claim, Additional isothermal processing applies a mechanical explosion as for under including method. 8. The 1st claim to there is in which one claim in the 7th claim, Whole taking effect Boulos 1 per minute about 0.2 to at about 80.deg.C average speed at the time of cooling as for height phase as for under including method. 9. The 1st claim to there is in which one claim in the 8th claim, After heating the phase which constructs whole taking effect Boulos in addition as for under including method. 10. The 1st claim to there is in which one claim in the 9th claim, Whole taking effect Boulos per 1 second about impeller tip per of 10 cm to 1 second about 240 cm (impeller tip) from speed as for under agitating phase in addition as for under including method. 11. The 1st claim to there is in which one claim in the 10th claim, Period preprocessing from the system where whole taking effect Boulos contains pressure with about 10 psi to about 150 psi or about 20 psi to about 150 psi or about 30 psi to about 150 psi or about 50 psi to about 150 psi at the time of maintenance as for height phase in addition as for under including method. 12. The 1st claim to there is in which one claim in the 11th claim, Period preprocessing, to the system where whole taking effect Boulos contains the salt about under existing, to system about 0.01 M to the ion robbery of about 2 M as for under creating method. 13. The 1st claim to there is in which one claim in the 12th claim, The Yu Ji-seong microbe as for bacteriolysis height phase in addition about under including, the product oil small and the residues which are emitted (debris) more than 80% of bulk 0.1 .micro.m as for under exceeding the cow which has the size of diameter and residues particle size distribution as for under creating method. 14. The 1st claim to there is in which one claim in the 13th claim, The Yu Ji-seong microbe as for bacteriolysis height phase in addition about under including, the product oil small is emitted and more than 95% of residues bulk 0.1 .micro.m as for under exceeding the cow which has the size of diameter and residues particle size distribution as for under creating method. 15. There is in a 14th claim, As for under exceeding mixes 120 cm/s from impeller tip speed with the oil and the cell residues small as on continuation as for under frequency phase in addition the low of including method. 16. The 1st claim to there is in which one claim in the 15th claim, 30 minutes for additions to about 8 hours heats taking effect Boulos from more than 90.deg.C with taking effect Boulos which passed a preprocessing could amalgamate the method. 17. The 1st claim to there is in which one claim in the 16th claim, Extraction process metal in whole taking effect Boulos compared with oil at ratio more than of 2 at the time of concentration as for height method. 18. The 1st claim to there is in which one claim in the 17th claim, Will fix tightly about the sugar content method the low of executing phase.  In order to collect the petroleum and the whole the biomass which is constructed (biomass) and/or the solvent as for under using about under extraction technique and comparison the petroleum where the metal and the weapon element are fewer as for under frequency phase as for under including method. 19. The 1st claim to there is in which one claim in the 18th claim, Whole taking effect Boulos as for under exceeding the salt and the ion and was assembled will be fixed tightly 0.05 g liter at consistency the sugar content source as for under including method. 20. There is in a 19th claim, The salt and the ion Na, K and Ca, Mg, Zn and the chloride and the sulfate, the method which is chosen from the army which is composed of the phosphate, the lead nitrate, and these union water. 21. There is in a 19th claim, Salt and ion potassium, calcium or these union water as for under including method. 22. The 19th claim to there is in which one claim in the 21st claim, The salt and the ion at consistency of 0.5 to 40 g liter the method which becomes the enemy. 23. The 19th claim to there is in which one claim in the 22nd claim, Salt and ion compared with natrium potassium of high concentration as for under including method. 24. The 19th claim to there is in which one claim in the 22nd claim, Salt and ion 1 g liter as for under exceeding at consistency calcium as for under including method. 25. The 19th claim to there is in which one claim in the 23rd claim, Salt and ion 2.5 g liter as for under exceeding at consistency potassium as for under including method. 26. The 19th claim to there is in which one claim in the 25th claim, When the product is emitted from the Yu Ji-seong microbe, the method which helps the oil coat frequency the salt and the ion by a fusion. 27. There is in a 26th claim, Taking effect Boulos period fusion salt and ion of 0.5 to 40 g liter consistency as for under including method. 28. There is in a 26th claim, The fusion, more than 80% of the geological features bulk which amalgamates 40 .micro.m as for under exceeding has the size of diameter, the geological features particle size distribution which amalgamates as for under creating method. 29. There is in a 26th claim, The fusion, more than 95% of the geological features bulk which amalgamates 40 .micro.m as for under exceeding has the size of diameter, the geological features particle size distribution which amalgamates as for under creating method. 30. The 1st claim to there is in which one claim in the 29th claim, After heating taking effect Boulos at the time of decompression as for height phase.  And to cool whole taking effect Boulos, to before additional processing the solid person in Boulos at the time of concentration as for height phase in addition as for under including method. 31. The 1st claim to there is in which one claim in the 30th claim, After heating the solvent to taking effect Boulos which became the dry cell or the bacteriolysis about under adding the blend as for under forming phase in addition as for under including method. 32. There is in a 31st claim, The solvent the hexane, also space and space, the diesel, from the army which is composed of the alcohol, and these union water as for under selecting method. 33. The 31st claim or there is in a 32nd claim, The blend of taking effect Boulos which became the bacteriolysis and the solvent about under agitating the oil from the Yu Ji-seong microbe and to contact, as for under this extracting phase in addition as for under including method. 34. There is in a 33rd claim, The solvent and the oil from taking effect Boulos which became the bacteriolysis as for under separating phase in addition as for under including method. 35. There is in a 34th claim, In order to separate the solvent and the oil from taking effect Boulos which became the bacteriolysis the centrifugal machine as for under using phase as for under including method. 36. The 34th claim or there is in a 35th claim, The solvent and the oil to react, above of some of the oil with fuel ingredient at the time of conversion as for height phase in addition as for under including method. 37. There is in a 36th claim, The solvent and the remainder oil, with the fuel which included Bio fuel at the time of conversion as for height phase in addition as for under including method. 38. The 34th claim to there is in which one claim in the 37th claim, After use Boulos crops fertilizer, animal feed, yeast extract, yeast hydrolysis water or carbon/nutrients as source as for under using phase in addition as for under including method. 39. The 1st claim to there is in which one claim in the 38th claim, Whole taking effect Boulos where the Yu Ji-seong microbe contains the sugar content feedstock as for under including method. 40. There is in a 39th claim, Whole taking effect Boulos where the Yu Ji-seong microbe and the sugar content feedstock contain, taking effect Boulos 1 per liter about 50 to the geological features of about 250 g, per taking effect Boulos 1 liter about 0 to the sugar content of about 50 g, per taking effect Boulos 1 liter about 0 to will become the salt and taking effect Boulos 1 per liter about 10 of about 40 g to solid color of about 100 g, (lipid-free) dry biomass as for under including method. 41. The 1st claim to there is in which one claim in the 40th claim, Yu Ji-seong microbe 40 weight % region of above as for under including method. 42. The 1st claim to there is in which one claim in the 41st claim, Whole taking effect Boulos where the Yu Ji-seong microbe contains as for under pasteurization at a low temperature phase in addition as for under including method. 43. There is in a 42nd claim, About 1 minute to about for 3 hours heats whole taking effect Boulos with about 40.deg.C to about 80.deg.C with whole taking effect Boulos as for under pasteurization at a low temperature phase the low of including method. 44. There is in a 43rd claim, Whole taking effect Boulos about 90.deg.C to about 150.deg.C or about 100.deg.C to about 150.deg.C or about 110.deg.C to about 150.deg.C or about 120.deg.C to about 150.deg.C or about 130.deg.C to from about 150.deg.C temperature about 30 minutes to about 18 hours, or 3 timeout to about 18 hours, or 3 timeout to for about 8 hours at the time of maintenance as for height phase in addition as for under including method. 45. There is in a 44th claim, Period heating period the phase which sterling (stirring) does whole taking effect Boulos in addition as for under including method. 46. The 44th claim or there is in a 45th claim, To whole taking effect Boulos mountain as for under adding phase in addition as for under including method. 47. The 44th claim to there is in which one claim in the 46th claim, To whole taking effect Boulos salty taste as for under adding phase in addition as for under including method. 48. The 44th claim to there is in which one claim in the 47th claim, Bead wheat (bead mill), homogenizer and orifice plate (orifice plate), - through leaflet mixer, press, extruder, pressure explosion, wet milling, dry milling or other leaflet or mechanical explosion equipment more than 1 time whole taking effect Boulos at the time of passage as for height phase in addition as for under including method. 49. There is in a 48th claim, Bead wheat, homogenizer and orifice plate, - through leaflet mixer, press, extruder, pressure explosion, wet milling, dry milling or other leaflet or mechanical explosion equipment more than 2 times whole taking effect Boulos at the time of passage as for height phase as for under including method. 50. The 48th claim or there is in a 49th claim, Taking effect Boulos which became the bacteriolysis in courage from about 70.deg.C to about 100.deg.C about 1 to as for under sterling for about 60 hours phase in addition as for under including method. 51. There is in a 50th claim, To taking effect Boulos which became the bacteriolysis in courage the salt as for under adding phase in addition as for under including method. 52. There is in a 51st claim, About 2 weight % salt below as for under adding phase as for under including method. 53. The 51st claim or there is in a 52nd claim, The salt is, or NaCl, KCl and K [2] SO [4], Na [2] S0 [4], or more than one NaOH and KOH + H [2] SO [from the union water of 4] the method which is originated. 54. The 50th claim to there is in which one claim in the 53rd claim, In order to regulate with about 3 to about 11 ph of taking effect Boulos which became the bacteriolysis in courage salty taste as for under adding phase in addition as for under including method. 55. The 51st claim to there is in which one claim in the 54th claim, The oil where the profitable fatty acid is 20% under from whole taking effect Boulos through centrifugation as for under separating phase in addition as for under including method. 56. The 1st claim to there is in which one claim in the 55th claim, The method where the Yu Ji-seong microbe is the Yu Ji-seong yeast cell. 57. The 1st claim to there is in which one claim in the 56th claim, In order to break the Yu Ji-seong cell wall of the Yu Ji-seong microbe the union water of the enzyme the low of using in addition includes a phase, and is an uncle where here enzyme is the amylase and 1 40,000 presbyopia, and 1 30,000 presbyopia the uncle which as for under including method. 58. There is in a 57th claim, More than one cadence enzyme where the union water of the enzyme is selected from the clear statement other uncle, the protease, and Kitty or the army which is composed with the uncle in addition as for under including method. 59. The 57th claim or there is in a 58th claim, The amylase alpha 1-4 about the writing base course which is connected the method which is unique. 60. The 57th claim to there is in which one claim in the 59th claim, Union water of enzyme about 5 weight % to about 30 weight % amylase as for under including method. 61. The 57th claim to there is in which one claim in the 60th claim, The union water of the enzyme about 5 weight % to about 45 weight % the uncle which is 1 40,000 presbyopia as for under including method. 62. The 57th claim to there is in which one claim in the 61st claim, The union water of the enzyme about 5 weight % to about 45 weight % the uncle which is 1 30,000 presbyopia as for under including method. 63. The 57th claim to there is in which one claim in the 62nd claim, After breaking the Yu Ji-seong cell wall, from the Yu Ji-seong cell wall within the cell the metabolic product as for under collecting phase in addition as for under including method. 64. There is in a 63rd claim, Within cell metabolic product geological features as for under including method. 65. The 63rd claim or there is in a 64th claim, Within cell metabolic product with Bio fuel at the time of conversion as for height phase in addition as for under including method. 66. The 63rd claim to there is in which one claim in the 65th claim, After within the cell collecting the metabolic product, remain the hydrogenous extraction effluence which is at the time of recirculation as for height phase in addition as for under including method. 67. There is in a 66th claim, In order to extract the sugar content the process feedstock as for under washing the quantity of swelling (imbibition water) as the number of extractions which recirculate as for under using phase in addition as for under including method. 68. Is an uncle which is a amylase and 1 40,000 presbyopia, and 1 30,000 presbyopia the uncle which the low of including disjoints the microbe cell wall the enzyme union water for. 69. There is in a 68th claim, The amylase alpha 1-4 about the writing base course which is connected the enzyme union water which is unique. 70. The 68th claim or there is in a 69th claim, More than one cadence enzyme which is selected from the clear statement other uncle, the protease, and Kitty or the army which is composed with the uncle in addition as for under including the enzyme union water. 71. The 68th claim to there is in which one claim in the 70th claim, The union water with the [su] gun Dio ball loose para three usui (Sporidiobolus pararoseus) with MK29404 the enzyme union water which is used. 72. The 68th claim to there is in which one claim in the 71st claim, About 5 weight % to about 30 weight % amylase as for under including enzyme union water. 73. The 68th claim to there is in which one claim in the 72nd claim, About 5 weight % to about 45 weight % the uncle which is 1 40,000 presbyopia as for under including the enzyme union water. 74. The 68th claim to there is in which one claim in the 73rd claim, About 5 weight % to about 45 weight % the uncle which is 1 30,000 presbyopia as for under including the enzyme union water. 75. Is an uncle which is a amylase and 1 40,000 presbyopia to whole taking effect Boulos where the Yu Ji-seong microbe contains, and 1 30,000 presbyopia the uncle which as for under including the union water of the enzyme about under applying the cell wall of the Yu Ji-seong microbe as for under breaking phase.  And Succeeding from the Yu Ji-seong microbe the product the low of extracting phase As for under including, from whole taking effect Boulos the suitable geological features extraction method which produces Bio fuel. 76. There is in a 75th claim, More than one cadence enzyme where the union water of the enzyme is selected from the clear statement other uncle, the protease, and Kitty or the army which is composed with the uncle in addition as for under including method. 77. The 75th claim or there is in a 76th claim, The amylase alpha 1-4 about the writing base course which is connected the method which is unique. 78. The 75th claim to there is in which one claim in the 77th claim, Union water of enzyme about 5 weight % to about 30 weight % amylase as for under including method. 79. The 75th claim to there is in which one claim in the 78th claim, The union water of the enzyme about 5 weight % to about 45 weight % the uncle which is 1 40,000 presbyopia as for under including method. 80. The 75th claim to there is in which one claim in the 79th claim, The union water of the enzyme about 5 weight % to about 45 weight % the uncle which is 1 30,000 presbyopia as for under including method. 81. The 75th claim to there is in which one claim in the 80th claim, After breaking the cell wall, from the Yu Ji-seong microbe within the cell the metabolic product as for under collecting phase in addition as for under including method. 82. There is in a 81st claim, Within cell metabolic product geological features as for under including method. 83. The 81st claim or there is in a 82nd claim, Within cell metabolic product with Bio fuel at the time of conversion as for height phase in addition as for under including method. 84. The 81st claim to there is in which one claim in the 83rd claim, After within the cell collecting the metabolic product, remain the hydrogenous extraction effluence which is at the time of recirculation as for height phase in addition as for under including method. 85. There is in a 84th claim, In order to extract the sugar content the process feedstock as for under washing from the swelling waterway the number of extractions which recirculate as for under using phase in addition as for under including method. 86. The 75th claim to there is in which one claim in the 85th claim, About 90.deg.C to about 150.deg.C or about 100.deg.C to about 150.deg.C or about 110.deg.C to about 150.deg.C or about 120.deg.C to about 150.deg.C or about 130.deg.C to heats whole taking effect Boulos at about 150.deg.C temperature with as for under preprocessing phase in addition the low of including method. 87. The 75th claim to there is in which one claim in the 86th claim, The solvent to taking effect Boulos which became the dry cell or the bacteriolysis about under adding the blend as for under forming phase in addition as for under including method. 88. There is in a 87th claim, The solvent the hexane, also space and space, the diesel, from the army which is composed of the alcohol, and these union water as for under selecting method. 89. The 87th claim or there is in a 88th claim, The blend of Boulos and the solvent about under agitating from a Yu Ji-seong yeast three prisoner of war the oil and to contact, as for under this extracting phase in addition as for under including method. 90. There is in a 89th claim, Solvent and oil from Boulos as for under separating phase in addition as for under including method. 91. There is in a 90th claim, In order from Boulos to separate the solvent and the oil the centrifugal machine as for under using phase as for under including method. 92. The 90th claim or there is in a 91st claim, The solvent and the oil to react, above of some of the oil with fuel ingredient at the time of conversion as for height phase in addition as for under including method. 93. There is in a 92nd claim, The solvent and the remainder oil, with the fuel which included Bio fuel at the time of conversion as for height phase in addition as for under including method. 94. The 75th claim to there is in which one claim in the 93rd claim, After use Boulos crops fertilizer, animal feed, yeast extract, yeast hydrolysis water or carbon/nutrients as source as for under using phase in addition as for under including method. 95. From hydrogenous taking effect Boulos where the Yu Ji-seong microbe contains the geological features about under extracting, the phase which leaves the biomass solid person and the remainder Boulos water.  And In order to extract the sugar content the process feedstock as for under washing from the swelling waterway the minute description remainder Boulos water as for under using phase As for under including, from hydrogenous taking effect Boulos the suitable geological features extraction method which produces Bio fuel. 96. There is in a 95th claim, Hydrogenous taking effect Boulos as for under pasteurization at a low temperature phase in addition as for under including method. 97. There is in a 96th claim, About period below 1 minutes to about 3 hours heats hydrogenous taking effect Boulos with about 40.deg.C to about 80.deg.C with hydrogenous taking effect Boulos as for under pasteurization at a low temperature phase the low of including method. 98. The 95th claim to there is in which one claim in the 97th claim, Hydrogenous taking effect Boulos about 30 minutes to about 18 hours, or 3 timeout to about 18 hours, or 3 timeout to as for under heating for about 8 hours phase as for under including method. 99. There is in a 98th claim, Hydrogenous taking effect Boulos about 90.deg.C to about 150.deg.C or about 100.deg.C to about 150.deg.C or about 110.deg.C to about 150.deg.C or about 120.deg.C to about 150.deg.C or about 130.deg.C to from about 150.deg.C temperature about 30 minutes to about 18 hours, or 3 timeout to about 18 hours, or 3 timeout to for about 8 hours at the time of maintenance as for height phase in addition as for under including method. 100. There is in a 99th claim, Period heating period hydrogenous taking effect Boulos as for under sterling phase in addition as for under including method. 101. The 95th claim to there is in which one claim in the 100th claim, To hydrogenous taking effect Boulos mountain as for under adding phase in addition as for under including method. 102. The 95th claim to there is in which one claim in the 101st claim, To hydrogenous taking effect Boulos salty taste as for under adding phase in addition as for under including method. 103. The 95th claim to there is in which one claim in the 102nd claim, Bead wheat, homogenizer and orifice plate, - through leaflet mixer, press, extruder, pressure explosion, wet milling, dry milling or other leaflet or mechanical explosion equipment more than 1 time hydrogenous taking effect Boulos at the time of passage as for height phase in addition as for under including method. 104. There is in a 103rd claim, Bead wheat, homogenizer and orifice plate, - through leaflet mixer, press, extruder, pressure explosion, wet milling, dry milling or other leaflet or mechanical explosion equipment more than 2 times hydrogenous taking effect Boulos at the time of passage as for height phase as for under including method. 105. The 95th claim to there is in which one claim in the 104th claim, Taking effect Boulos which became the bacteriolysis in courage from about 70.deg.C to about 100.deg.C about 1 to as for under sterling for about 60 hours phase in addition as for under including method. 106. There is in a 105th claim, To taking effect Boulos which became the bacteriolysis in courage the salt as for under adding phase in addition as for under including method. 107. There is in a 106th claim, To taking effect Boulos which became the bacteriolysis in courage about 2 weight % the salt below as for under adding phase as for under including method. 108. Are in the 106th claims or 107 claims, The salt NaCl or Na [2] S0 [4] the method. 109. The 105th claim to there is in which one claim in the 108th claim, Salty taste about under adding ph of taking effect Boulos which became the bacteriolysis in courage the phase which regulates with about 3 to about 11 in addition as for under including method. 110. The 95th claim to there is in which one claim in the 109th claim, The geological features from taking effect Boulos which became the bacteriolysis through centrifugation as for under separating phase in addition as for under including method. 111. The 95th claim to there is in which one claim in the 110th claim, Hydrogenous taking effect Boulos sugar cane extract as for under including method. 112. The 95th claim to there is in which one claim in the 110th claim, The biomass solid person by the remaining Boulos water at the time of recirculation as for height phase in addition as for under including method.
法律状态
(KR20160101990) LEGAL DETAILS FOR KR20160101990  Actual or expected expiration date=2034-12-18    Legal state=ALIVE    Status=PENDING     Event publication date=2014-12-18  Event code=KR/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=KR KR20167019377  Application date=2014-12-18  Standardized application number=2016KR-7019377     Event publication date=2016-08-26  Event code=KR/A  Event type=Examination events  Published application  Publication country=KR  Publication number=KR20160101990  Publication stage Code=A  Publication date=2016-08-26  Standardized publication number=KR20160101990
专利类型码
A
国别省市代码
若您需要申请原文,请登录。

最新评论

暂无评论。

登录后可以发表评论

意见反馈
返回顶部