一种​牛樟​芝​银耳​保健​口服液​的​制备​方法

源语言标题
(CN105995992) 一种​牛樟​芝​银耳​保健​口服液​的​制备​方法
公开号/公开日
CN105995992 A 2016-10-12 [CN105995992] / 2016-10-12
申请号/申请日
2016CN-0360653 / 2016-05-28
发明人
LU GUOXIAO; LI LIANG
申请人
LU GUOXIAO
主分类号
IPC分类号
A23L-033/00;C12N-001/14;C12R-001/645
摘要
(CN105995992)
本​发明​公开​了​种​牛樟​芝​银耳​保健​口服液​的​制备​方法,属于​食品加工​领域。其​特征​在于:采用​牛樟​芝​斜面​菌种​培养、二级​液体​摇床​培养​牛樟​芝​菌丝​液​提取​银耳​提取​液​制备​混合​浓缩​调配​均质​灌装​封盖​杀菌​包装​检验​成品​的​制作​工艺流程。有益​效果:本​发明​产品​色泽​呈​棕褐色,均匀​致,风味​独特;组织​形态​呈​浑浊​液,香甜​可口,具有​牛樟​芝​和​银耳​特有​的​清香​风味。本​产品​营养​丰富,可以​调节、增强​人体​免疫力,还​具有​补​肺​益​肾、抗​肿瘤、抗​过敏、降​血压、降​胆固醇、保护​肝脏​等​多种​保健​功效。老少皆宜,绿色​保健。
地址
代理人
代理机构
优先权号
2016CN-0360653 2016-05-28
主权利要求
(CN105995992)
1. Preparation of antrodia mesenteric a health drink solution of bovine method, characterized in: using camphorata ramp seed cultureone, secondary liquid shaking culturecamphorata hypha liquid extractionmesenteric prepared extractmixedconcentratedformulatedhomogeneousfillingclosuresterilizingpackagingtestthe finished making process flow, the following specific steps: Camphorata (1) ramp seed culture: antrodia chicago parent species will be under sterile conditions, by contact with the sweet potato, gluten meal, PDA medium composed of glucose and agar, and at 30-32 Ć incubated for 45-50 days, mycelia grown over a ramp to be, i.e. to an expanded camphorata ramp species; A two-stage (2) liquid shaking culture: media formulations to 30% sweet potato, 0.2% potassium phosphate monobasic, 0.1% ammonium sulfate, 0.1% citric acid, water 800 milliliters; aliquots at the medium 300 milliliters Erlenmeyer flask, bottle 160 milliliters per, 1 with 3 layers of gauze and a layer of kraft paper seal, in the 1.049x105 Pa, 45 minutes and sterilized at 108 Ć; and then the enlarged camphorata ramp species access primary liquid in shake flasks, each flask to spawn 2-2.5 square centimeter, at 35 Ć, 300 revolutions/min for shake culture was performed 3-5 days, when the shake flask incubation was terminated by the appearance of multiple small hyphae ball, and the culture medium access a secondary liquid, inoculation was 22%, at a first shake flasks shake culture under the same conditions 2-4 days, Light bee honey when the fermentation fluids is, clear, and numerous white hyphae culturing the ball is stopped; Camphorata (3) hypha liquid extraction: 0.1% to 0.1% of the amylase and the fermentation broth composite proteases in 45 Ć, pH was 5 enzymatic treatment is carried out at 1-3 hours, then washed with 100 holes/square centimeter of the copper mesh filter, to give camphorata mycelia extract; Mesenteric (4) prepared extract: optional premium impurity-free dry silver ears entity, medlar, immersed in 20 - fold dilution in the liquid milk, soaking 2-4 hours, longer set as a high speed beater grinding, made mesenteric a slurry; adjusted to pH slurry of the mesenteric 6-7, added mass fraction of from 1% composite protease, temperature-controlled at 40-42 Ć, 45 minutes digestion, enzyme inactivation temperature was raised to 98 Ć is then carried out, hot water leaching 2 pass, the combined leach solution, filtered before use; Mixed (5), concentrated: camphorata mycelia extract will, mesenteric uniformly mixed at a ratio 1:2 through was extracted, concentrated in vacuo injections of low temperature in the tank, to be soluble solids  75%, pH was 5-6 it is possible to; Deployment (6), homogeneous: 35 MPa feed through the high pressure homogenizer treatment, the material to be miniaturized, formed having a diameter of 0.1 micrometer a small molecule, and adding an appropriate amount of glue, lily juice and a protein powder; Filling (7) sterilization: a mixture of the nutrient solution bottle filled with 350 milliliters after brown stock solution is homogeneous, the cap sterilization-after, sterilizing the formula is: 10 minutes-15 minutes-25 minutes/108 Ć, verified, labeling, packaging is given by the product.
法律状态
(CN105995992)
LEGAL DETAILS FOR CN105995992

Actual or expected expiration date=2036-05-28   
Legal state=ALIVE   
Status=PENDING 



Event publication date=2016-05-28 
Event code=CN/APP 
Event indicator=Pos 
Event type=Examination events 
Application details

Application country=CN CN201610360653 
Application date=2016-05-28 
Standardized application number=2016CN-0360653 



Event publication date=2016-10-12 
Event code=CN/A 
Event type=Examination events 
Published application

Publication country=CN 
Publication number=CN105995992 
Publication stage Code=A 
Publication date=2016-10-12 
Standardized publication number=CN105995992 



Event publication date=2016-10-12 
Event code=CN/C06 
Event indicator=Pos 
Event type=Examination events 
Publication



Event publication date=2016-12-21 
Event code=CN/C10 
Event type=Examination events 
Request of examination as to substance
专利类型码
A
国别省市代码
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