(US6638910) The present invention relates to a novel antibiotic-producing and metabolite-producing Bacillus subtilis strain that exhibits insecticidal, antifungal and antibacterial activity. The supernatant of this novel strain contains effective insecticidal, antifungal and antibacterial agents. Also included in the invention is a solvent extractable, small molecular weight (<10,000 daltons) corn rootworm-active metabolite produced in the supernatant. Also included in the invention are methods of protecting or treating plants from fungal and bacterial infections and corn rootworm infestations comprising the step of applying to the plant an effective amount of the antibiotic/metabolite-producing novel Bacillus subtilis strain, the antibiotic/metabolite produced by the novel Bacillus subtilis strain or a combination thereof, optionally further comprising another antibiotic-producing bacterial strain and/or a chemical pesticide. The invention also includes methods of preventing or treating fungal and bacterial infections using whole broth cultures or supernatants obtained from cultures of the novel Bacillus subtilis stain alone or in combination with chemical pesticides and/or other biocontrol agents. The invention also includes novel antifungal and antibacterial compounds designated agrastatins and a novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin. Methods of treating or protecting plants from fungal and bacterial infections and corn rootworm infestations comprising administering the novel agrastatins and the novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin are provided. Further provided is a lipopeptide extract isolated from strain AQ713 with insecticidal activity and a surfactin lipopeptide isolated from strain AQ713 with insecticidal activity.
(US6638910) 1. A composition for protecting or treating plants and fruit from fungal and bacterial infections and corn rootworm infestations comprising effective amounts of an iturin A, a plipastatin, a surfactin and an agrastatin. What is claimed is: 2. The composition of claim 1, further comprising a chemical fungicide. 3. The composition of claim 1, further comprising a different biological or chemical pesticide or insecticide. 4. The composition of claim 1, further comprising a chemical fungicide. 5. The composition of claim 1, wherein the composition is formulated as a powder, a granule, or a flowable (liquid). 6. The composition of claim 1, wherein the composition is microencapsulated. 7. A method for protecting or treating plants and/or fruit from fungal and bacterial infections and corn root worm infestations comprising applying an effective amount of any of the compositions of claims 1 or 2 to 6 to the plants and/or fruit. 8. The method of claim 7, wherein the infections are caused by at least one microorganism selected from the group consisting of Phytophthora infestans, Rhizoctonia solani, Pythium ultimum, Botrytis cinerea, Alternaria solani, Colletotrichum cocodes, Alternaria brassicicola, Cladosprorium cucumerinum, Monilinia fructicola, Venturia pyrina, Acidovarax avanae, Pseudomonas syringae, Xanthomonas campestris, Erwinia carotovora, Clavibacter michiganense, Plasmopara viticola, Sphaerotheca fuliginea, Uncinula necator, and Peronospora parasitica. 9. The method of claim 7, wherein the composition comprised of an iturin A, a plipastatin, a surfactin and an agrastatin is applied as wettable powders, granules, flowables or microencapsulations. 10. A method for protecting or treating plants corn rootworm infestations comprising applying an effective amount of any of the compositions of claims 1 or 2 to 3 to roots of plants or to soil around the roots.
(US6638910) LEGAL DETAILS FOR US2003186852 Actual or expected expiration date=2018-12-30 Legal state=ALIVE Status=GRANTED Event publication date=2001-11-27 Event code=US/APP Event indicator=Pos Event type=Examination events Application details Application country=US US09994234 Application date=2001-11-27 Standardized application number=2001US-09994234 Event publication date=2001-11-27 Event code=US/EXMR Event type=Administrative notifications USPTO Examiner Name Primary Examiner: TATE, CHRISTOPHER ROBIN Event publication date=2001-11-27 Event code=US/ART Event type=Administrative notifications USPTO Art Group ART=1654 Event publication date=2001-11-27 Event code=US/DK Event type=Examination events Attorney Docket Number Docket Nbr: AQ 2009.01 Event publication date=2001-11-27 Event code=US/ENT Event type=Administrative notifications Entity Status Set to Undiscounted Business Entity Status: UNDISCOUNTED Event publication date=2001-11-27 Event code=US/AIA Event type=Administrative notifications First Inventor File Indicated: AIA=No Event publication date=2001-11-27 Event code=US/APE Event type=Corrections Preliminary amendments Event publication date=2002-05-21 Event code=US/APE Event type=Corrections Preliminary amendments Event publication date=2002-07-23 Event code=US/DOCK Event indicator=Pos Event type=Examination events Case Docketed to Examiner Event publication date=2002-10-04 Event code=US/DOCK Event indicator=Pos Event type=Examination events Case Docketed to Examiner Event publication date=2002-10-07 Event code=US/CTNF Event type=Examination events Event type=OA Event type=OAO Non-Final Rejection Event publication date=2003-01-14 Event code=US/IDS Event type=Examination events Event type=OAI Information Disclosure Statement Filed Event publication date=2003-01-14 Event code=US/CTNFR Event type=Examination events Event type=OAI Response after Non-Final Action Event publication date=2003-02-19 Event code=US/PET Event type=APL Petition Entered Event publication date=2003-03-24 Event code=US/POAC Event type=Change of name or address Change in Power of Attorney (May Include Associate POA) Event publication date=2003-03-28 Event code=US/EXAC Event type=Examination events Event type=OAO Examiner's Amendment Communication Event publication date=2003-04-14 Event code=US/NOAM Event indicator=Pos Event type=Examination events Event type=OAO Mail Notice of Allowance Event publication date=2003-05-21 Event code=US/IDS Event type=Examination events Event type=OAI Information Disclosure Statement Filed Event publication date=2003-06-24 Event code=US/EXAC Event type=Examination events Event type=OAO Examiner's Amendment Communication Event publication date=2003-09-12 Event code=US/APRDY Event indicator=Pos Event type=Examination events Application Is Considered Ready for Issue Event publication date=2003-10-02 Event code=US/A1 Event type=Examination events Application published Publication country=US Publication number=US2003186852 Publication stage Code=A1 Publication date=2003-10-02 Standardized publication number=US20030186852 Event publication date=2003-10-09 Event code=US/PAT Event indicator=Pos Event type=Event indicating In Force Patented Case Event publication date=2003-10-09 Event code=US/ISSM Event indicator=Pos Event type=Examination events Event type=OAO Event type=Restitution or restoration Issue Notification Mailed Event publication date=2003-10-28 Event code=US/B2 Event indicator=Pos Event type=Event indicating In Force Granted patent as second publication Publication country=US Publication number=US6638910 Publication stage Code=B2 Publication date=2003-10-28 Standardized publication number=US6638910 Event publication date=2003-10-28 Event code=US/354 Event indicator=Pos Event type=Extension of term of duration of protection Patent term extended under 35 U.S.C 154(b) until/for Delays (A,B,C): 0 Overlap Delays: 0 Non Overlap Delays: 0 PTO Office Delays: 0 Applicant Delays: 7 Adjustment total: Number of days of extension=0 Event publication date=2004-03-17 Event code=US/POAC Event type=Change of name or address Change in Power of Attorney (May Include Associate POA) Event publication date=2006-11-07 Event code=US/NMFP Event type=Payment or non-payment notifications Publication of First Notice of Maintenance Fees Payable. 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