(WO200993254) The present invention provides methods and compositions for identifying subjects at risk of developing a complication of pregnancy, such as preeclampsia or preterm labor. The compositions are microRNAs and associated nucleic acids.
(WO200993254) CLAIMS 1. A method of determining a physiological condition in a subject, said method comprising detecting the level of a microRNA in a serum sample obtained from the subject, wherein a level of the microRNA different from a control is indicative of said physiological condition in said subject. 2. The method of claim 1, wherein the physiological condition is a pregnancy-associated disorder. 3. The method of claim 2, wherein the pregnancy- associated disorder is preeclampsia or preterm labor. 4. The method of claim 1, wherein the detection of the microRNA level is determined by real-time PCR. 5. A method for determining or aiding in the determination that a female subject is at risk of developing preeclampsia, comprising comparing the expression profile of a nucleic acid sequence selected from the group consisting of SEQ ID NOS: 5-17, a fragment thereof and a sequence having at least about 80% identical thereto in a biological sample from the subject to be assessed for risk of developing preeclampsia to a predetermined standard expression profile, wherein a significant difference in expression profile of said nucleic acid sequence in the sample as compared to a predetermined standard expression profile indicates that the subject is at risk of developing preeclampsia. 6. The method of claim 5, wherein the predetermined standard expression profile corresponds to the expression profile of said nucleic acid sequence in a pregnant female subject who is not at risk of developing preeclampsia. 7. The method of claim 5, wherein said biological sample is selected from the group consisting of bodily fluid and a tissue sample. 8. The method of claim 7, wherein said tissue is a fresh, frozen, fixed, wax- embedded or formalin fixed paraffin-embedded (FFPE) tissue. 9. The method of claim 8, wherein said tissue sample is placenta sample or uterine myometrium sample. 10. The method of claim 7, wherein said bodily fluid sample is serum sample. 11. The method of claim 5, wherein the method comprises determining the expression levels of at least two nucleic acid sequences. 12. The method of claim 5, wherein the method further comprises combining one or more expression ratios of said nucleic acid sequences. 13. The method of claim 5, wherein the expression levels are determined by a method selected from the group consisting of nucleic acid hybridization and nucleic acid amplification. 14. The method of claim 13, wherein the nucleic acid hybridization is performed using a solid-phase nucleic acid biochip array or in situ hybridization. 15. The method of claim 13, wherein the nucleic acid amplification method is real-time PCR. 16. A method for detecting or monitoring a preterm labor in a female subject, comprising comparing the expression profile of a nucleic acid sequence selected from the group consisting of SEQ ID NOS: 18-25, a fragment thereof and sequence having at least about 80% identity thereto in a biological sample from the subject to a predetermined standard expression profile, wherein a significant difference in the expression profile of said nucleic acid sequence in the sample as compared to a predetermined expression profile standard indicates that the subject is in preterm labor. 17. The method of claim 16, wherein the predetermined standard expression profile corresponds to the expression profile of said nucleic acid sequence in a pregnant female subject who is not at risk of preterm labor. 18. A kit for determining if a female subject is at risk of developing preeclampsia, said kit comprising a probe comprising a nucleic acid sequence that is complementary to a sequence selected from the group consisting of SEQ ID NOS: 5-17, a fragment thereof and a sequence having at least about 80% identity thereto. 19. A kit for determining if a female subject is at risk of having a preterm labor, said kit comprising a probe comprising a nucleic acid sequence that is complementary to a sequence selected from the group consisting of SEQ ID NOS: 18-25, a fragment thereof and a sequence having at least about 80% identity thereto. 20. The kit of any of claims 18-19, wherein the kit further comprises forward and reverse primers.
(WO200993254) LEGAL DETAILS FOR WO2009093254 Actual or expected expiration date=2011-07-27 Legal state=DEAD Status=LAPSED Event publication date=2009-01-26 Event code=WO/APP Event indicator=Pos Event type=Examination events Application details Application country=WO WOIL2009000101 Application date=2009-01-26 Standardized application number=2009WO-IL00101 Event publication date=2009-07-30 Event code=WO/A2 Event type=Examination events International application published with declaration under Article 17 (2) (a) Publication country=WO Publication number=WO2009093254 Publication stage Code=A2 Publication date=2009-07-30 Standardized publication number=WO200993254 Event publication date=2010-03-11 Event code=WO/A3 Event indicator=Pos Event type=Examination events Later publication of ISR with revised front page Publication country=WO Publication number=WO2009093254 Publication stage Code=A3 Publication date=2010-03-11 Standardized publication number=WO200993254 Event publication date=2011-07-27 Event code=WO/EETL Event type=Event indicating Not In Force PCT Application validity period expired. 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