Methods and compositions for diagnosing complications of pregnancy 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
WO2009093254 A2 2009-07-30 [WO200993254]WO2009093254 A3 2010-03-11 [WO200993254] / 2009-07-302010-03-11
申请号/申请日
2009WO-IL00101 / 2009-01-26
发明人
GOREN YARON;GILAD SHLOMIT;HOD MOSHE;YOGEV YARIV;
申请人
MOR RESEARCH APPLICATIONS;ROSETTA GENOMICS;
主分类号
IPC分类号
A01N-043/04A61K-031/70C12Q-001/68
摘要
(WO200993254) The present invention provides methods and compositions for identifying subjects at risk of developing a complication of pregnancy, such as preeclampsia or preterm labor.  The compositions are microRNAs and associated nucleic acids.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2008US-61023859 2008-01-27 2008US-61040671 2008-03-30
主权利要求
(WO200993254) CLAIMS 1. A method of determining a physiological condition in a subject, said method comprising detecting the level of a microRNA in a serum sample obtained from the subject, wherein a level of the microRNA different from a control is indicative of said physiological condition in said subject. 2. The method of claim 1, wherein the physiological condition is a pregnancy-associated disorder. 3. The method of claim 2, wherein the pregnancy- associated disorder is preeclampsia or preterm labor. 4. The method of claim 1, wherein the detection of the microRNA level is determined by real-time PCR. 5. A method for determining or aiding in the determination that a female subject is at risk of developing preeclampsia, comprising comparing the expression profile of a nucleic acid sequence selected from the group consisting of SEQ ID NOS: 5-17, a fragment thereof and a sequence having at least about 80% identical thereto in a biological sample from the subject to be assessed for risk of developing preeclampsia to a predetermined standard expression profile, wherein a significant difference in expression profile of said nucleic acid sequence in the sample as compared to a predetermined standard expression profile indicates that the subject is at risk of developing preeclampsia. 6. The method of claim 5, wherein the predetermined standard expression profile corresponds to the expression profile of said nucleic acid sequence in a pregnant female subject who is not at risk of developing preeclampsia. 7. The method of claim 5, wherein said biological sample is selected from the group consisting of bodily fluid and a tissue sample. 8. The method of claim 7, wherein said tissue is a fresh, frozen, fixed, wax- embedded or formalin fixed paraffin-embedded (FFPE) tissue. 9. The method of claim 8, wherein said tissue sample is placenta sample or uterine myometrium sample. 10. The method of claim 7, wherein said bodily fluid sample is serum sample. 11. The method of claim 5, wherein the method comprises determining the expression levels of at least two nucleic acid sequences. 12. The method of claim 5, wherein the method further comprises combining one or more expression ratios of said nucleic acid sequences. 13. The method of claim 5, wherein the expression levels are determined by a method selected from the group consisting of nucleic acid hybridization and nucleic acid amplification. 14. The method of claim 13, wherein the nucleic acid hybridization is performed using a solid-phase nucleic acid biochip array or in situ hybridization. 15. The method of claim 13, wherein the nucleic acid amplification method is real-time PCR. 16. A method for detecting or monitoring a preterm labor in a female subject, comprising comparing the expression profile of a nucleic acid sequence selected from the group consisting of SEQ ID NOS: 18-25, a fragment thereof and sequence having at least about 80% identity thereto in a biological sample from the subject to a predetermined standard expression profile, wherein a significant difference in the expression profile of said nucleic acid sequence in the sample as compared to a predetermined expression profile standard indicates that the subject is in preterm labor. 17. The method of claim 16, wherein the predetermined standard expression profile corresponds to the expression profile of said nucleic acid sequence in a pregnant female subject who is not at risk of preterm labor. 18. A kit for determining if a female subject is at risk of developing preeclampsia, said kit comprising a probe comprising a nucleic acid sequence that is complementary to a sequence selected from the group consisting of SEQ ID NOS: 5-17, a fragment thereof and a sequence having at least about 80% identity thereto. 19. A kit for determining if a female subject is at risk of having a preterm labor, said kit comprising a probe comprising a nucleic acid sequence that is complementary to a sequence selected from the group consisting of SEQ ID NOS: 18-25, a fragment thereof and a sequence having at least about 80% identity thereto. 20. The kit of any of claims 18-19, wherein the kit further comprises forward and reverse primers.
法律状态
(WO200993254) LEGAL DETAILS FOR WO2009093254  Actual or expected expiration date=2011-07-27    Legal state=DEAD    Status=LAPSED     Event publication date=2009-01-26  Event code=WO/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=WO WOIL2009000101  Application date=2009-01-26  Standardized application number=2009WO-IL00101     Event publication date=2009-07-30  Event code=WO/A2  Event type=Examination events  International application published with declaration under Article 17 (2) (a)  Publication country=WO  Publication number=WO2009093254  Publication stage Code=A2  Publication date=2009-07-30  Standardized publication number=WO200993254     Event publication date=2010-03-11  Event code=WO/A3  Event indicator=Pos  Event type=Examination events  Later publication of ISR with revised front page  Publication country=WO  Publication number=WO2009093254  Publication stage Code=A3  Publication date=2010-03-11  Standardized publication number=WO200993254     Event publication date=2011-07-27  Event code=WO/EETL  Event type=Event indicating Not In Force  PCT Application validity period expired. LEGAL DETAILS FOR DESIGNATED STATE AU2009207291  Actual or expected expiration date=2029-01-26    Legal state=ALIVE    Status=GRANTED   Corresponding cc:  Designated or member state=AU Corresponding appl: AU2009207291  Application date in the designated or member state=2009-01-26   Application number in the designated or member state=2009AU-0207291 Corresponding cc:  Designated or member state=AU Corresponding pat: AU2009207291  Publication stage code in the designated or member state=A1  Publication date in the designated or member state=2009-07-30   Publication number in the designated or member state=AU2009207291    Event publication date=2010-08-26  Event code=WO/ENP  Event type=Entry into national phase  Entry into the national phase in: Corresponding cc:  Designated or member state=AU     Event publication date=2015-03-05  Event code=AU/STCHG  Patent Status changed by the national office Corresponding cc:  Designated or member state=AU  LEGAL DETAILS FOR DESIGNATED STATE 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expiration date=2010-07-28    Legal state=DEAD    Status=LAPSED   Corresponding cc:  Designated or member state=DE     Event publication date=2010-07-28  Event code=WO/NENP  Event type=Event indicating Not In Force  Non-entry into the national phase in: Corresponding cc:  Designated or member state=DE  LEGAL DETAILS FOR DESIGNATED STATE EP2234483  Actual or expected expiration date=2011-10-25    Legal state=DEAD    Status=LAPSED   Corresponding cc:  Designated or member state=EP Corresponding appl: EP09703376  Application date in the designated or member state=2009-01-26   Application number in the designated or member state=2009EP-0703376 Corresponding cc:  Designated or member state=EP Corresponding pat: EP2234483  Publication stage code in the designated or member state=A2  Publication date in the designated or member state=2010-10-06   Publication number in the designated or member state=EP2234483    Event publication date=2009-09-23  Event code=WO/121  Event type=Designated states 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Corresponding cc:  Designated or member state=IL  LEGAL DETAILS FOR DESIGNATED STATE JP2011510623  Actual or expected expiration date=2029-01-26    Legal state=ALIVE    Status=PENDING   Corresponding cc:  Designated or member state=JP Corresponding appl: JP2010543627  Application date in the designated or member state=2009-01-26   Application number in the designated or member state=2010JP-0543627 Corresponding cc:  Designated or member state=JP Corresponding pat: JP2011510623  Publication stage code in the designated or member state=A  Publication date in the designated or member state=2011-04-07   Publication number in the designated or member state=JP2011510623    Event publication date=2010-07-26  Event code=WO/WWE  Event indicator=Pos  Event type=Entry into national phase  Wipo information: entry into national phase Corresponding cc:  Designated or member state=JP  LEGAL DETAILS FOR DESIGNATED STATE US2010291585  Actual or expected expiration date=2030-05-17    Legal state=ALIVE   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专利类型码
A2A3
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