Colicin m for the control of ehec 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
EP3097783 A1 2016-11-30 [EP3097783] / 2016-11-30
申请号/申请日
2015EP-0181133 / 2015-08-14
发明人
GIRITCH ANATOLI;HAHN SIMONE;SCHULZ STEVE;STEPHAN ANETT;GLEBA YURI;JARCZOWSKI FRANZISKA;
申请人
NOMAD BIOSCIENCE;
主分类号
IPC分类号
A01N-063/02A01P-001/00
摘要
(EP3097783) The invention provides a method of preventing or reducing contamination of an object such as food with enterohaemorrhagic E . coli (EHEC), comprising contacting said object with colicin M or a derivative thereof.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2015US-62166379 2015-05-26
主权利要求
(EP3097783) 1. A method of preventing or reducing contamination of an object such as food with enterohaemorrhagic E. coli (EHEC), comprising contacting said object with colicin M or a derivative thereof. 2. The method according to claim 1, wherein contamination of an object such as food with EHEC serotype O157:H7 is prevented or reduced. 3. The use according to claim 1, wherein contamination of an object such as food with any one or all of the following E. coli serotypes is prevented or reduced: serotype 026:H11, serotype O45:H2, serotype O103:H11, serotype O111:H8, serotype O157:H7, and serotype O104:H4. 4. The use according to claim 1, wherein contamination of an object such as food with any one or all of the following E. coli serotypes is prevented or reduced: serotype 026:H11, serotype O45:H2, serotype O103:H11, serotype O111:H8, serotype O145:NM, O157:H7, and O104:H4. 5. The method according to claim 1, wherein said object is contacted with an aqueous solution containing colicin M or its derivative by spraying with said aqueous solution or by dipping said object into said aqueous solution. 6. The method according to claim 1, wherein said food is immersed for at least 10 seconds, preferably for at least 1 minute, preferably for at least 5 minutes into an aqueous solution of colicin M or its derivative. 7. The method according to claim 1, wherein said colicin M or its derivative is produced by expression in a plant or in plant cells, followed by removing undesired components from said plant or said plant cells. 8. The method according to claim 1, wherein said food is meat, raw fruit or raw vegetable. 9. The method according to claim 1, wherein said colicin M has the amino acid sequence of SEQ ID NO: 1. 10. The method according to claim 1, wherein the toxicity of the derivative of colicin M is such that the derivative and the colicin M of SEQ ID NO: 1 produce spots free of viable bacteria of sensitive E. coli strain DH10B of the same diameter 12 hours after spotting 5 microliters of a solution of said derivative of colicin M and the colicin M of SEQ ID NO: 1 onto a lawn of the sensitive E. coli strain on an agar plate and subsequent incubation of the agar plate at 37 deg.C, wherein the concentration of th e derivative of colicin M is at most 5 times that of the comparative solution of the colicin M of SEQ ID NO: 1. 11. The method according to claim 1, wherein said derivative of colicin M comprises the C-terminal activity domain of residues 141 to 271 of colicin M or an activity domain having from 1 to 30, preferably from 1 to 20, amino acid substitutions, insertions, additions and/or deletions compared to residues 141 to 271 of SEQ ID NO: 1. 12. The method according to claim 1, wherein said derivative of colicin M comprises the central receptor-binding domain of residues 36 to 140 of colicin M or an activity domain having from 1 to 10 amino acid substitutions, insertions additions and/or deletions compared to residues 36 to 140 of SEQ ID NO: 1. 13. The method according to claim 1, wherein said colicin M or its derivative has amino acid residues 1 to 35 of SEQ ID NO: 1 or has from 1 to 8, preferably from 1 to 4, amino acid substitutions, insertions, additions and/or deletions compared to amino acid residues 1 to 35 of SEQ ID NO: 1. 14. The method according to claim 1, wherein said colicin M or its derivative is used in combination with one or more colicins selected from the group consisting of colE7, colB, colla, colU, colK, col5, and colE7, preferably said colicin M or its derivative is used in combination with colE7. 15. The method according to claim 14, wherein contamination with any one or all of the following E. coli serotypes is prevented or reduced: serotypes 026:H11, O45:H2, 0103:H11, 0111:H8, O145:NM, O157:H7, O104:H4, and O121:H19.
法律状态
(EP3097783) LEGAL DETAILS FOR EP3097783  Actual or expected expiration date=2035-08-14    Legal state=ALIVE    Status=PENDING     Event publication date=2015-08-14  Event code=EP/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=EP EP15181133  Application date=2015-08-14  Standardized application number=2015EP-0181133     Event publication date=2016-11-30  Event code=EP/A1  Event type=Examination events  Application published with search report  Publication country=EP  Publication number=EP3097783  Publication stage Code=A1  Publication date=2016-11-30  Standardized publication number=EP3097783     Event publication date=2016-11-30  Event code=EP/AK  Event indicator=Pos  Event type=Designated states  Designated contracting states: Benannte vertragsstaaten AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR    Event publication date=2016-11-30  Event code=EP/AV  Event indicator=Pos  Event type=Designated states  Request for validation of the European patent in MA    Event publication date=2016-11-30  Event code=EP/AX  Event indicator=Pos  Event type=Designated states  Extension of the European patent to Request for extension of the european patent to BA ME    Event publication date=2017-07-05  Event code=EP/17P  Event indicator=Pos  Event type=Examination events  Request for examination filed Pruefungsantrag gestellt  Effective date of the event=2017-05-29     Event publication date=2017-07-05  Event code=EP/RBV  Event indicator=Pos  Event type=Designated states  Event type=Corrections  Designated contracting states (correction): Benannte vertragsstaaten (korr.) AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR    Event publication date=2017-08-23  Event code=EP/17Q  Event indicator=Pos  Event type=Examination events  First examination report Erster pruefungsbescheid  Effective date of the event=2017-07-24     Event publication date=2017-11-15  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Inventor changed before grant    Event publication date=2017-11-15  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Inventor changed before grant    Event publication date=2017-11-15  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Inventor changed before grant    Event publication date=2017-11-15  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Inventor changed before grant    Event publication date=2017-11-15  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Inventor changed before grant    Event publication date=2017-11-15  Event code=EP/RIN1  Event type=Change of name or address  Event type=Administrative notifications  Inventor (correction) Inventor changed before grant
专利类型码
A1
国别省市代码
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