Polymethyl methacrylate (PMMA) point of care for ABO-Rh(D) blood typing 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
Sensors and Actuators, B. Chemical
年/卷/期
2018 / 273 /
页码
703-709
ISSN号
0925-4005
作者单位
Mahidol Univ, Ramathibodi Hosp, Dept Pathol, Fac Med, Bangkok 10400, Thailand;Mahidol Univ, Mat Sci & Engn Program, Ctr Mat Sci & Innovat, Bangkok 10400, Thailand;Burapha Univ, Fac Engn, Off Educ, Chon Buri 20131, Thailand;King Mongkuts Univ Technol Norh, Dept Ind Phys & Med Instrumentat, Fac Sci Appl, Bangkok 10800, Thailand;King Mongkuts Univ Technol Norh, Dept Ind Phys & Med Instrumentat, Fac Sci Appl, Bangkok 10800, Thailand;Suranaree Univ Technol, Sch Elect Engn, Inst Engn, Nakhon Ratchasima 30000, Thailand;Mahidol Univ, Ramathibodi Hosp, Dept Pathol, Fac Med, Bangkok 10400, Thailand;
作者
Kunakorn, Mongkol;Srikhirin, Toemsak;Pipatpanukul, Chinnawut;Kataphiniharn, Chanwit;Wangkam, Thidarat;Sutapun, Boonsong;Kitpoka, Pimpun;
摘要
Polymethyl methacrylate (PMMA) based point of care (POC) for ABO-Rh(D) blood grouping by the naked eye was investigated. PMMA substrate was covalently linked with carboxyl methyl dextran (CMD) and coupled to the blood group specific antibodies anti A, anti B, and anti D, which were patterned into "A", "B", and "+". The red read out color originated from the color of hemoglobin in the ABCs. The major human blood group, ABO-Rh (D), was successfully sorted using these patterns. PMMA strip-based assays for blood grouping by the naked eye resulted in correct blood group identification of all 74 samples in comparison to slide agglutination test. The determination of captured RBC Rh(D) required a longer reaction time with immobilized anti D antibody on the PMMA substrate than other blood groups to produce sufficient color for unambiguous visual identification. The proper RBC concentration was found to be higher than 1% v/v for naked eye detection. The dilution of blood sample is playing a crucial role in reducing interference from blood proteins during detection. A used of undiluted blood sample for the testing should be avoided. The proper blood dilution was found to be 10% or 1:10 dilution. The proposed technique presents a quick, cheap, simple, and almost instantaneous assay for blood grouping.
机翻摘要
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关键词/主题词
Blood grouping;POC;PMMA;Strip test;Surface modification;
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