Method for producing an L-amino acid 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2013260425 A1 2013-10-03 [US20130260425]US8951760 B2 2015-02-10 [US8951760] / 2013-10-032015-02-10
申请号/申请日
2013US-13912313 / 2013-06-07
发明人
DOI HIDETAKA;HOSHINO YASUSHI;MASUMITSU YURI;USUDA YOSHIHIRO;
申请人
AJINOMOTO;
主分类号
IPC分类号
C07K-014/245C12N-001/20C12N-001/38C12N-015/74C12P-013/04C12P-013/08
摘要
(US8951760) A method for producing an L-amino acid includes culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium.  A bacterium which has been subjected to a modification including at least one of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof, is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.
机翻摘要
暂无翻译结果,您可以尝试点击头部的翻译按钮。
地址
代理人
代理机构
;
优先权号
2010JP-0276062 2010-12-10 2011WO-JP78380 2011-12-08
主权利要求
(US8951760) We claim: 1.  A method for producing an L-amino acid, the method comprising: A) culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability, in a medium containing a carbon source selected from the group consisting of a fatty acid and an alcohol;   B) reducing intracellular hydrogen peroxide concentration of the bacterium; and   C) collecting the L-amino acid from the medium,    wherein said reducing is accomplished by modifying the bacterium by increasing the copy number of a fixABC gene, and/or replacing a promoter of a fixABC gene with a stronger promoter. 2. The method according to claim 1, wherein the bacterium belongs to the genus Escherichia, Pantoea, or Enterobacter. 3. The method according to claim 2, wherein the bacterium is Escherichia coli, Pantoea ananatis, or Enterobacter aerogenes. 4. The method according to claim 1, wherein fixABC encodes proteins comprising the amino acid sequences of SEQ ID NOS: 11, 13, and 15, or conservative variants of these sequences, wherein said conservative variants have the amino acid sequences of SEQ ID NOS: 11, 13, and 15 but include 1 to 5 amino acid substitutions, deletions, or insertions, and wherein the conservative variants have a function for reducing intracellular hydrogen peroxide concentration of the bacterium. 5. The method according to claim 1, wherein the carbon source is a fatty acid. 6. The method according to claim 5, wherein the fatty acid is oleic acid. 7. The method according to claim 5, wherein the fatty acid is a mixture of fatty acids derived from a fat or oil. 8. The method according to claim 1, wherein the carbon source is an alcohol. 9. The method according to claim 8, wherein the alcohol is glycerol. 10. The method according to claim 8, wherein the alcohol is ethanol. 11. The method according to claim 1, wherein the carbon source is a mixture of a fatty acid and glycerol obtained by hydrolyzing a fat or oil. 12. The method according to claim 10, wherein the bacterium is Escherichia coli which has been modified to be able to aerobically utilize ethanol. 13. The method according to claim 1, wherein the L-amino acid is L-lysine. 14. The method according to claim 13, further comprising: A) enhancing an activity of at least one enzyme selected from the group consisting of dihydrodipicolinate reductase, diaminopimelate decarboxylase, diaminopimelate dehydrogenase, phosphoenolpyruvate carboxylase, aspartate aminotransferase, diaminopimelate epimerase, aspartate semialdehyde dehydrogenase, tetrahydrodipicolinate succinylase, and succinyldiaminopimelate deacylase, wherein the activity of the enzyme is enhanced by increasing the copy number of a gene coding the enzyme, and/or replacing a promoter of a gene coding the enzyme with a stronger promoter; or   B) disrupting lysine decarboxylase; or   C) both.
法律状态
(US8951760) LEGAL DETAILS FOR US2013260425  Actual or expected expiration date=2031-12-08    Legal state=ALIVE    Status=GRANTED     Event publication date=2013-06-07  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US13912313  Application date=2013-06-07  Standardized application number=2013US-13912313     Event publication date=2013-06-28  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment OWNER: AJINOMOTO CO., INC., JAPAN ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:DOI, HIDETAKA HOSHINO, YASUSHI MASUMITSU, YURI AND OTHERS SIGNING DATES FROM 20130618 TO 20130621 REEL/FRAME:030710/0874     Event publication date=2013-10-03  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2013260425  Publication stage Code=A1  Publication date=2013-10-03  Standardized publication number=US20130260425     Event publication date=2015-02-10  Event code=US/B2  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as second publication  Publication country=US  Publication number=US8951760  Publication stage Code=B2  Publication date=2015-02-10  Standardized publication number=US8951760
专利类型码
A1B2
国别省市代码
若您需要申请原文,请登录。

最新评论

暂无评论。

登录后可以发表评论

意见反馈
返回顶部