Recombinant chitinase and use thereof as a biocide 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US5866788 A 1999-02-02 [US5866788] / 1999-02-02
申请号/申请日
1995US-08524051 / 1995-09-06
发明人
KRAMER KARL J;MUTHUKRISHNAN SUBBARATNAM;CHOI HEE KYUNG;CORPUZ LOLITA;GOPALAKRISHNAN BHUVANA;
申请人
KANSAS STATE UNIVERSITY RESEARCH FOUNDATION;US DEPARTMENT OF AGRICULTURE;
主分类号
IPC分类号
A01N-063/00C12N-009/24C12N-015/12C12N-015/82
摘要
(US5866788) A recombinantly derived insect chitinase gene is inserted into a vector for host expression of the chitinase protein, and may be utilized according to methods for controlling a population of insect pests.  A recombinant virus is provided for this purpose, as well as a recombinant plant.  Recombinantly derived insect chitinase may also be expressed in host cells or larvae for subsequent purification.
机翻摘要
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地址
代理人
代理机构
;
优先权号
1994US-08224987 1994-04-08 1995US-08524051 1995-09-06
主权利要求
(US5866788) We claim: 14.  A transgenic plant having a genome comprising an oligonucleotide encoding an insect chitinase having insecticidal activity, said chitinase having an active site with the sequence of the amino acids 136-148 of Seq.  Id. No. 2. 1. An isolated oligonucleotide encoding an insect chitinase having insecticidal activity, said chitinase having an active site with the sequence of the amino acids 136-148 of SEQ.  ID. No. 2. 2. The oligonucleotide of claim 1, said chitinase having at least a 50% amino acid sequence similarity to the protein encoded by SEQ ID No. 1. 3. The digonucleotide of claim 1, said oligonucleotide encoding the protein encoded by Sequence ID No. 1. 4. The digonucleotide of claim 1, said oligonucleotide comprising the coding region of Sequence ID No. 1. 5. The oligonucleotide of claim 1, said oligonucleotide being a cDNA. 6. A chimeric vector comprising an oligonucleotide encoding an insect chitinase having insecticidal activity, said chitinase having an active site with the sequence of the amino acids 136-148 of Seq.  Id. No. 2. 7. The vector of claim 6, said chitinase having at least 50% amino acid sequence similarity to the protein encoded by Sequence ID No. 1. 8. The vector of claim 6, said oligonucleotide encoding the protein encoded by Sequence ID No. 1. 9. The vector of claim 6, said oligonucleotide comprising the coding region of Sequence ID No. 1. 10. The vector of claim 6, said oligonucleotide being a cDNA. 11. The vector of claim 6, said vector being an Agrobacterium tumefaciens vector. 12. The vector of claim 11, said oligonucleotide encoding the protein encoded by Sequence ID No. 1. 13. The vector of claim 11, said oligonucleotide comprising the coding region of Sequence ID No. 1. 15. The plant of claim 14, said chitinase having a least 50% amino acid sequence similarity to the protein encoded by SEQ ID No. 1. 16. The plant of claim 14, said oligonucleotide encoding the protein encoded by Sequence ID No. 1. 17. The plant of claim 14, said oligonucleotide comprising the coding region of Sequence ID No. 1. 18. The plant of claim 14, said plant being tobacco. 19. A method of controlling insect, fungal or nematode pests comprising the steps of: (a) growing a transgenic plant having a genome comprising an oligonucleotide encoding an insect chitinase having insecticidal activity, said chitinase having an active site with the sequence of the amino acids 136-148 of Seq.  Id. No. 2;  and (b) allowing said pests to eat portions of said plant. 20. The method of claim 19, said chitinase having at least 50% amino acid sequence similarity to the protein encoded by SEQ ID No. 1. 21. The method of claim 19, said oligonucleotide encoding the protein encoded by Sequence ID No. 1. 22. The method of claim 19, said oligonucleotide comprising the coding region of Sequence ID No. 1. 23. The method of claim 19, said plant being tobacco. 24. The plant of claim 14, said plant being a cereal grain. 25. The method of claim 19, said pests being insects. 26. The method of claim 19, said plant being a cereal grain. 27. An isolated gene from Manduca sexta which encodes insecticidal chitinase. 28. A transgenic plant having a genome comprising an oligonucleotide encoding the protein encoded by SEQ ID No. 1. 29. An isolated oligonucleotide encoding the protein encoded by SEQ ID No. 1. 30. An isolated oligonucleotide comprising the coding region of SEQ ID No. 1. 31. A chimeric vector comprising an oligonucleotide encoding an insect chitinase having insecticidal activity, said chitinase having an active site with the sequence of the amino acids 136-148 of Seq.  Id. No. 2, said vector being the plasmid deposited as ATCC A No. 97248.
法律状态
(US5866788) LEGAL DETAILS FOR US5866788  Actual or expected expiration date=2007-03-07    Legal state=DEAD    Status=LAPSED     Event publication date=1995-09-06  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US08524051  Application date=1995-09-06  Standardized application number=1995US-08524051     Event publication date=1995-09-06  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: FOX, DAVID T    Event publication date=1995-09-06  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1649     Event publication date=1995-09-06  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: 22875-A    Event publication date=1995-09-06  Event code=US/SMALL  Event type=Administrative notifications  Appl Has Filed a Verified Statement of Micro to Small Entity Status Business Entity Status: SMALL    Event publication date=1995-09-06  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=1996-04-24  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=1996-05-06  Event code=US/M327  Event type=Examination events  Event type=OAO  Miscellaneous Communication to Applicant - No Action Count    Event publication date=1996-06-28  Event code=US/APE  Event type=Corrections  Preliminary amendments    Event publication date=1996-09-16  Event code=US/APE  Event type=Corrections  Preliminary amendments    Event publication date=1997-01-06  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=1997-01-14  Event code=US/RESTREQ  Event type=Examination events  Event type=Corrections  Event type=OAO  Restriction/Election Requirement    Event publication date=1997-02-11  Event code=US/ELC  Event type=Examination events  Event type=OAI  Response to Election / Restriction Filed    Event publication date=1997-05-12  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=1997-09-16  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=1997-09-16  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=1997-12-15  Event code=US/CTFR  Event type=Examination events  Event type=OA  Event type=OAO  Final Rejection    Event publication date=1998-04-13  Event code=US/ABNEA  Event indicator=Neg  Event type=Event indicating Not In Force  Event type=OAI  Expressly Abandoned -- During Examination    Event publication date=1998-04-13  Event code=US/APE  Event type=Corrections  Preliminary amendments    Event publication date=1998-04-13  Event code=US/ACPA  Event type=Examination events  Event type=CPA  Continued Prosecution Application - Continuation (ACPA)    Event publication date=1998-04-13  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=1998-04-24  Event code=US/EXIN  Event type=Examination events  Event type=OAO  Event type=INT  Examiner Interview Summary Record (PTOL - 413)    Event publication date=1998-04-28  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=1999-01-07  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Event type=Restitution or restoration  Issue Notification Mailed    Event publication date=1999-02-02  Event code=US/A  Event indicator=Pos  Event type=Event indicating In Force  Patents Granted before 2001-04-15  Publication country=US  Publication number=US5866788  Publication stage Code=A  Publication date=1999-02-02  Standardized publication number=US5866788     Event publication date=2002-02-12  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. PAYMENT NOTICE YEAR:  Year of payment of annual fees=3     Event publication date=2002-08-01  Event code=US/FPAY  Event indicator=Pos  Event type=Event indicating In Force  Event type=Payment or non-payment notifications  Fee payment recorded   Annual fees payment date=2002-08-01     Year of payment of annual fees=4     Event publication date=2002-08-27  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: KANSAS STATE UNIVERSITY RESEARCH FOUNDATION, KANSA  Effective date of the event=2002-08-14  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNOR:KANSAS STATE UNIVERSITY RESEARCH FOUNDATION REEL/FRAME:013221/0615     Event publication date=2002-08-27  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: UNITED STATES DEPARTMENT OF AGRICULTURE, MASSACHUS  Effective date of the event=2002-08-14  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNOR:KANSAS STATE UNIVERSITY RESEARCH FOUNDATION REEL/FRAME:013221/0615     Event publication date=2006-02-14  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. PAYMENT NOTICE YEAR:  Year of payment of annual fees=7     Event publication date=2006-08-23  Event code=US/REMI  Event type=Administrative notifications  Maintenance fee reminder mailed    Event publication date=2007-02-02  Event code=US/FP  Event indicator=Neg  Event type=Event indicating Not In Force  Event type=Payment or non-payment notifications  Expired due to failure to pay maintenance fee  Effective date of the event=2007-02-02     Event publication date=2007-02-02  Event code=US/LAPS  Event indicator=Neg  Event type=Event indicating Not In Force  Event type=Payment or non-payment notifications  Lapse for failure to pay maintenance fees    Event publication date=2007-03-07  Event code=US/FP  Event indicator=Neg  Event type=Event indicating Not In Force  Event type=Payment or non-payment notifications  Expired due to failure to pay maintenance fee    Event publication date=2007-03-07  Event code=US/EXP  Event indicator=Neg  Event type=Event indicating Not In Force  Patent expired    Event publication date=2010-02-16  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. PAYMENT NOTICE YEAR:  Year of payment of annual fees=11
专利类型码
A
国别省市代码
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