Transgenic strains for biocontrol of plant root diseases 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US6277625 B1 2001-08-21 [US6277625] / 2001-08-21
申请号/申请日
1997US-08994035 / 1997-12-18
发明人
HUANG ZHENGYU;THOMASHOW LINDA S;MAVRODI DMITRI V;RAAIJMAKERS JOS M;WELLER DAVID M;COOK R JAMES;
申请人
WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION;
主分类号
IPC分类号
A01N-063/00C12N-001/00C12N-001/20C12N-001/21C12N-015/09C12N-015/52C12N-015/78
摘要
(US6277625) Transgenic fluorescent Pseudomonas spp. are described which have a biosynthetic locus which encodes for the production of the antibiotic phenazine-1-carboxylic acid stably introduced into the genome, have a locus which encodes for the production of the antibiotic 2,4-diacetylphloroglucinol, and are effective for control of diseases caused by the soil-borne fungus, Rhizoctonia.  Strains are also described which control diseases caused by Gaeumannomyces graminis or Pythium, in addition to Rhizoctonia, or have the ability to control all three diseases.
机翻摘要
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地址
代理人
代理机构
;
优先权号
1997US-08974938 1997-11-20 1997US-08994035 1997-12-18
主权利要求
(US6277625) What is claimed is: 1.  A biologically pure culture of a strain of fluorescent Pseudomonas spp. bacteria which has a biosynthetic locus which encodes for the production of 2,4-diacetylphloroglucinol, which strain has been stably transformed with a DNA sequence which encodes for the biosynthesis of phenazine-1-carboxylic acid, whereby said transformed strain has the ability to produce both 2,4-diacetylphloroglucinol and phenazine-1-carboxylic acid. 2. The biologically pure culture of claim 1 which is further characterized as having bands at 600 bp +- 50 bp;  700 +- 50 bp;  800 bp +- 50 bp;  900 bp +- 50 bp identified by Random Amplified Polymorphic DNA (RAPD) analysis using primer M13. 3. The biologically pure culture of claim 2 which is further characterized as having bands at 330 bp +- 20 bp and 1100 bp +- 60 bp. 4. The biologically pure culture of claim 1 wherein said strain is further characterized as having the ability to suppress root diseases in plants caused by the fungus Rhizoctonia. 5. The biologically pure culture of claim 4 wherein said strain is further characterized as having the ability to suppress root diseases in plants caused by the fungi Gaeumannomyces graminis (Gg) or Pythium. 6. The biologically pure culture of claim 1 wherein said fluorescent Pseudomonas strain has all of the identifying characteristics of P. fluorescens NRRL B-21905, NRRL B-21906, NRRL B-21907 or NRRL B-21908. 7. The biologically pure culture of claim 1 which further includes an agricultural carrier. 8. A method of controlling a root disease caused by Rhizoctonia, Pythium, or Gaeumannomyces graminis fungus (Gg) in plants susceptible to said root disease, which comprises growing said plant in the presence of an effective biocontrol amount of a biologically pure culture of a fluorescent Pseudomonas strain of claim 1. 9. The method of claim 8 wherein said plant is selected from the group consisting of small grain crop, turf grass or food, fiber or ornamental plant. 10. The method of claim 8 wherein seed of said plant is treated with an effective biocontrol amount of said strain prior to said growing. 11. The method of claim 8, wherein soil or furrow for growing said plant is treated with an effective biocontrol amount of said strain prior to said growing. 12. The method of claim 8 wherein said plant is treated with a bacterial treatment solution which comprises an effective biocontrol amount of said strain and a suitable liquid carrier. 13. The method of claim 8 wherein said Pseudomonas strain is a P. fluorescens strain having all of the identifying characteristics of NRRL B-21905, NRRL B-21906, NRRL B-21907 or NRRL B21908. 14. The method of claim 10 wherein said seed has a concentration of about 102 to 105 CFU per seed. 15. The method of claim 12 wherein said treatment solution has a concentration about 108 to 1010 CFU per ml of solution. 16. The method of claim 8 wherein roots of said plant are dipped into a bacterial suspension of 102 to 105 CFU per ml of suspension. 17. An agricultural composition for controlling a root disease caused by Rhizoctonia, Pythium, or Gaeumannomyces graminis fungus (Gg) in plants susceptible to said root disease, said composition comprising a suitable carrier and an effective biocontrol amount of a biologically pure culture of a fluorescent Pseudomonas strain of claim 1. 18. The agricultural composition of claim 17 wherein said carrier is selected from the group consisting of water, buffer, methylcellulose, peat, and vermiculite. 19. The agricultural composition of claim 18 wherein said strain is in a concentration of about 108 to 1010 CFU per ml of liquid carrier or 107 to 109 per gram of solid carrier. 20. The agricultural composition of claim 17 wherein said Pseudomonas strain is a P. fluorescens strain having all of the identifying characteristics of NRRL B-21905, NRRL B-21906, NRRL B-21907 or NRRL B- 21908. 21. A seed of a plant having applied thereto an effective biocontrol amount of a biologically pure culture of claim 1. 22. The seed of claim 21 where said seed has a concentration of about about 102 to 105 CFU per seed. 23. A stain of fluorescent Pseudomonas spp. which naturally produces 2,4-diacetylphloroglucinol, which strain has been transformed by stable introduction of a DNA sequence which encodes for the biosynthesis of phenazine-1-caroxylic acid, whereby said transformed strain has the ability to produce both 2,4-diacetylphloroglucinol and phenazine-1-carboxylic acid.
法律状态
(US6277625) LEGAL DETAILS FOR US6277625  Actual or expected expiration date=2013-09-16    Legal state=DEAD    Status=LAPSED     Event publication date=1997-12-18  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US08994035  Application date=1997-12-18  Standardized application number=1997US-08994035     Event publication date=1997-12-18  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: ZAGHMOUT, OUSAMA M    Event publication date=1997-12-18  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1638     Event publication date=1997-12-18  Event code=US/ENT  Event type=Administrative notifications  Entity Status Set to Undiscounted Business Entity Status: UNDISCOUNTED    Event publication date=1997-12-18  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=1997-12-18  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: 0009.98    Event publication date=1997-12-18  Event code=US/CUST  Event type=Examination events  Attorney/Agent Customer Number Customer Nbr: 25278    Event publication date=1998-02-27  Event code=US/INCD  Event type=Examination events  Event type=OAO  Notice Mailed--Application Incomplete--Filing Date Assigned    Event publication date=1998-04-24  Event code=US/APE  Event type=Corrections  Preliminary amendments    Event publication date=1998-08-04  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=1998-09-21  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION, W  Effective date of the event=1998-09-16  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNOR:MAVRODI, DMITRI V. REEL/FRAME:009470/0137     Event publication date=1999-02-16  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=1999-05-06  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=1999-08-17  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=1999-08-17  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=2000-01-10  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2000-03-22  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=2000-05-11  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=2000-05-11  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=2000-08-14  Event code=US/SUPRSP  Event type=OAI  Supplemental Response    Event publication date=2000-12-27  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=2001-07-22  Event code=US/APRDY  Event indicator=Pos  Event type=Examination events  Application Is Considered Ready for Issue    Event publication date=2001-08-06  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Event type=Restitution or restoration  Issue Notification Mailed    Event publication date=2001-08-21  Event code=US/B1  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as first publication  Publication country=US  Publication number=US6277625  Publication stage Code=B1  Publication date=2001-08-21  Standardized publication number=US6277625     Event publication date=2005-02-22  Event code=US/FPAY  Event indicator=Pos  Event type=Event indicating In Force  Event type=Payment or non-payment notifications  Fee payment recorded   Annual fees payment date=2005-02-22     Year of payment of annual fees=4     Event publication date=2009-03-03  Event code=US/FPAY  Event indicator=Pos  Event type=Event indicating In Force  Event type=Payment or non-payment notifications  Fee payment recorded   Annual fees payment date=2009-03-03     Year of payment of annual fees=8     Event publication date=2009-03-03  Event code=US/SULP  Event indicator=Pos  Event type=Restitution or restoration  Event type=Event indicating In Force  Surcharge for late payment   Annual fees payment date=2009-03-03     Year of payment of annual fees=7     Event publication date=2013-04-01  Event code=US/REMI  Event type=Administrative notifications  Maintenance fee reminder mailed    Event publication date=2013-08-21  Event code=US/LAPS  Event indicator=Neg  Event type=Event indicating Not In Force  Event type=Payment or non-payment notifications  Lapse for failure to pay maintenance fees    Event publication date=2013-08-21  Event code=US/FP  Event indicator=Neg  Event type=Event indicating Not In Force  Event type=Payment or non-payment notifications  Expired due to failure to pay maintenance fee  Effective date of the event=2013-08-21     Event publication date=2013-09-13  Event code=US/EXP  Event indicator=Neg  Event type=Event indicating Not In Force  Patent expired    Event publication date=2013-09-16  Event code=US/FP  Event indicator=Neg  Event type=Event indicating Not In Force  Event type=Payment or non-payment notifications  Expired due to failure to pay maintenance fee
专利类型码
B1
国别省市代码
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