Encapsulation of material in microbial cells 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US5288632 A 1994-02-22 [US5288632] / 1994-02-22
申请号/申请日
1989US-07402347 / 1989-09-05
发明人
PANNELL NAHIDA A;
申请人
AD2;BESTFOODS;
主分类号
IPC分类号
A01N-025/28A23L-027/00A61K-009/50A61L-009/12B01J-013/02C12N-001/00
摘要
(US5288632) Microbial encapsulation of materials is carried out by mixing a microbe with an encapsulatable material in liquid form in an aqueous medium to form an aqueous emulsion of the encapsulatable material.  The encapsulatable material in the emulsion is absorbed into the microbe by diffusing across the microbe cell wall.  The microbe has a lipid content of less than 40% by weight such as up to about 5% and may contain about 50-75% of the encapsulatable material.  The emulsion is formed in the absence of a surfactant and the microbe is not treated with a lipid-extending substance or a plasmolyzer.  The encapsulatable material can have a benzene or naphthalene ring and may be a perfume, an insecticide or a drug.  If the material is normally solid, it can be dissolved in a solvent.  The microbe may be treated to enhance permeability prior to or during encapsulation.  After encapsulation, the microbe can be separated from the medium by centrifuging, freeze drying or spray-drying.  Material encapsulated can be released by rupture of the microbe cell wall.
机翻摘要
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地址
代理人
代理机构
;
优先权号
1986GB-0008964 1986-04-12 1987US-07037182 1987-04-10 1989US-07402347 1989-09-05
主权利要求
(US5288632) I claim: 1. Method for the production of a microbially encapsulated material, comprising: treating a grown intact microbe having a microbial lipid content of significantly less than 40% by weight, with an encapsulatable material in liquid form which is capable of diffusing into the microbial cell without causing total lysation thereof, said treatment comprising mixing the microbe with the encapsulatable material in the presence of an aqueous medium, but in the absence of added surfactant, to produce an aqueous emulsion of the encapsulatable material and to maintain the aqueous emulsion during the mixing, whereby the encapsulatable material is absorbed by the microbe by diffusion across the microbial cell wall and the encapsulatable material is retained passively within the microbe, wherein the encapsulatable material is not in aqueous emulsion form prior to said mixing of the microbe therewith, and the method being performed in the absence of treatment of the microbe with a lipid-extending substance or a plasmolyser. 2. Method according to claim 1 wherein the mixing is performed at an elevated temperature in the range 35 (degree)  C. to 60 (degree)  C., at least during the initial stage of the mixing. 3. Method according to claim 1 wherein the mixing is performed for a time until the desired optimum amount of one or more globules of the material can be observed microscopically within the microbial cell. 4. Method according to claim 3 wherein the resulting microbial capsule is harvested and then subjected to heat-treatment. 5. Method according to claim 1 wherein the resultant microbially encapsulated material is separated from the residual method ingredients by spray-drying or freeze-drying. 6. Method according to claim 1 wherein the microbe is selected from fungi, bacteria and algae. 7. Method according to claim 1 wherein the microbe is a filamentous fungus. 8. Method according to claim 1 wherein the microbe is alive at least at the commencement of the mixing. 9. Method according to claim 1 wherein the microbe is a fungus having a lipid content of up to about 5% by weight. 10. Method according to claim 1 wherein the microbe is a yeast. 11. Method according to claim 1 wherein the microbe is selected from Saccharomyces cerevisiae, Candida utilis, Kluyveromyces fragilis, Aspergillus niger, Fusarium graminearum and Fusarium rocqfortiae. 12. Method according to claim 1 wherein the microbe has an average cell diameter of greater than about 5 microns. 13. Method according to claim 1 wherein the encapsulatable material has a benzene or a naphthalene ring. 14. Method according to claim 1 wherein the encapsulatable material is selected from benzaldehyde, essential oils used in flavours or fragrances, pheromones, organophosphorus insecticidal compounds, leuco dyes, menthol, lauryl ether sulphate, alphachloralose, dichlorophen, onion extract, oil of wintergreen, and water-soluble food colourants. 15. A microbially encapsulated material when produced by the method according to claim 1. 16. Method for the production of a microbially encapsulated material, comprising: treating a grown intact microbe having a microbial lipid content of significantly less than 40% by weight, with an encapsulatable material in liquid form which is capable of diffusing into the microbial cell without causing total lysation thereof, the liquid form of the encapsulatable material being selected from encapsulatable materials which are liquids in their normal state and solutions and microdispersions of encapsulatable materials in an organic solvent or microdispersant therefor, said treatment comprising mixing the microbe with the encapsulatable material in the presence of an aqueous medium, but in the absence of added surfactant, to produce an aqueous emulsion of the encapsulatable material and to maintain the aqueous emulsion during the mixing, whereby the encapsulatable material is absorbed by the microbe by diffusion across the microbial cell wall and the encapsulatable material is retained passively within the microbe, wherein the encapsulatable material is not in aqueous emulsion form prior to said mixing of the microbe therewith, and the method being performed in the absence of treatment of the microbe with a lipid-extending substance or a plasmolyser.
法律状态
(US5288632) LEGAL DETAILS FOR US5288632  Actual or expected expiration date=2009-09-05    Legal state=DEAD    Status=EXPIRED     Event publication date=1989-09-05  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US07402347  Application date=1989-09-05  Standardized application number=1989US-07402347     Event publication date=1989-09-05  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: NAFF, DAVID M    Event publication date=1989-09-05  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1808     Event publication date=1989-09-05  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: WAK25425A    Event publication date=1989-09-05  Event code=US/ENT  Event type=Administrative notifications  Entity Status Set to Undiscounted Business Entity Status: UNDISCOUNTED    Event publication date=1989-09-05  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=1989-09-05  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment  Effective date of the event=1989-08-31  ASSIGNMENT OF ASSIGNORS INTEREST. ASSIGNOR:PANNELL, NAHIDA A. REEL/FRAME:005131/0254     Event publication date=1989-09-22  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=1989-11-20  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=1990-01-26  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=1990-05-29  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=1990-05-29  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=1990-05-29  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=1990-08-03  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=1990-12-03  Event code=US/EXIN  Event type=Examination events  Event type=OAO  Event type=INT  Examiner Interview Summary Record (PTOL - 413)    Event publication date=1990-12-07  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=1990-12-07  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=1991-01-09  Event code=US/SUPRSP  Event type=OAI  Supplemental Response    Event publication date=1991-03-08  Event code=US/CTFR  Event type=Examination events  Event type=OA  Event type=OAO  Final Rejection    Event publication date=1991-11-04  Event code=US/NOAPLF  Event type=APL  Notice of Appeal Filed    Event publication date=1991-11-04  Event code=US/CTFAR  Event type=Examination events  Event type=OAI  Response after Final Action    Event publication date=1991-11-04  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=1991-11-13  Event code=US/CTAV  Event type=OAO  Advisory Action (PTOL-303)    Event publication date=1992-01-31  Event code=US/APB  Event type=APL  Event type=OAI  Appeal Brief Filed    Event publication date=1992-01-31  Event code=US/136G  Event type=OAO  Request for Extension of Time - Granted    Event publication date=1992-04-02  Event code=US/APEA  Event type=APL  Event type=OAO  Examiner's Answer to Appeal Brief    Event publication date=1992-07-22  Event code=US/APE2  Event type=APL  2nd or Subsequent Examiner's Answer to Appeal Brief    Event publication date=1993-07-16  Event code=US/BPAIR  Event type=APL  BPAI Decision - Examiner Reversed    Event publication date=1993-08-31  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=1994-01-14  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Event type=Restitution or restoration  Issue Notification Mailed    Event publication date=1994-02-09  Event code=US/PAT  Event indicator=Pos  Event type=Event indicating In Force  Patented Case    Event publication date=1994-02-22  Event code=US/A  Event indicator=Pos  Event type=Event indicating In Force  Patents Granted before 2001-04-15  Publication country=US  Publication number=US5288632  Publication stage Code=A  Publication date=1994-02-22  Standardized publication number=US5288632     Event publication date=1997-02-25  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. PAYMENT NOTICE YEAR:  Year of payment of annual fees=3     Event publication date=1997-08-13  Event code=US/FPAY  Event indicator=Pos  Event type=Event indicating In Force  Event type=Payment or non-payment notifications  Fee payment recorded   Annual fees payment date=1997-08-13     Year of payment of annual fees=4     Event publication date=1998-08-21  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment  Effective date of the event=1997-11-24  MERGER ASSIGNOR:CPC INTERNATIONAL INC. REEL/FRAME:009396/0275     Event publication date=1999-10-26  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. PAYMENT NOTICE YEAR:  Year of payment of annual fees=3     Event publication date=2001-03-06  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. PAYMENT NOTICE YEAR:  Year of payment of annual fees=7     Event publication date=2001-09-18  Event code=US/REMI  Event type=Administrative notifications  Maintenance fee reminder mailed    Event publication date=2001-12-10  Event code=US/SULP  Event indicator=Pos  Event type=Restitution or restoration  Event type=Event indicating In Force  Surcharge for late payment   Annual fees payment date=2001-12-10     Year of payment of annual fees=7     Event publication date=2001-12-10  Event code=US/FPAY  Event indicator=Pos  Event type=Event indicating In Force  Event type=Payment or non-payment notifications  Fee payment recorded   Annual fees payment date=2001-12-10     Year of payment of annual fees=8     Event publication date=2005-03-01  Event code=US/FPAY  Event indicator=Pos  Event type=Event indicating In Force  Event type=Payment or non-payment notifications  Fee payment recorded   Annual fees payment date=2005-03-01     Year of payment of annual fees=12     Event publication date=2005-03-01  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. PAYMENT NOTICE YEAR:  Year of payment of annual fees=11     Event publication date=2009-09-05  Event code=US/EEDX  Event indicator=Neg  Event type=Event indicating Not In Force  Patent has expired
专利类型码
A
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