Molecular identification of yeast, lactic and acetic acid bacteria species during spoilage of tchapalo, a traditional sorghum beer from Cote d'Ivoire 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
World Journal of Microbiology & Biotechnology
年/卷/期
2018 / 34 / 11
ISSN号
0959-3993
作者单位
Univ Nangui Abrogoua, UFR STA, Lab Biotechnol & Microbiol Aliments, 02 BP 801, Abidjan 02, Cote Ivoire;Univ Nangui Abrogoua, UFR STA, Lab Biotechnol & Microbiol Aliments, 02 BP 801, Abidjan 02, Cote Ivoire;Univ Nangui Abrogoua, UFR STA, Lab Biotechnol & Microbiol Aliments, 02 BP 801, Abidjan 02, Cote Ivoire;Univ Nangui Abrogoua, UFR STA, Lab Biotechnol & Microbiol Aliments, 02 BP 801, Abidjan 02, Cote Ivoire;
作者
Attchelouwa, Constant K.;N'guessan, Florent K.;Ake, Francine M. D.;Dje, Marcellin K.;
摘要
Yeasts, lactic and acetic acid bacteria are responsible of microbial spoilage of alcoholic beverages. However species involved in deterioration of sorghum beer produced in Cote d'Ivoire has not been investigated. This study was carried out to identify species of yeast, LAB and AAB during spoilage of tchapalo in order to define the best strategy for beer preservative. Thus, a total of 210 yeasts, LAB and AAB were isolated from samples of tchapalo stored at ambient temperature and at 4 degrees C for 3days. Based on PCR-RFLP of the ITS region and the sequencing of D1/D2 domain, yeast isolates were assigned to seven species (Saccharomyces cerevisiae, Candida tropicalis, Rhodotorula mucilaginosa, Trichosporon asahii, Kluyveromyces marxianus, Meyerozyma guilliermondii and Trichosporon coremiiforme). During the storage at ambient temperature and at 4 degrees C, S. cerevisiae was the predominant species (>76%). Excepted R. mucilaginosa, occurrence of non-Saccharomyces species was sporadic. LAB species detected in fresh samples using molecular methods were Pediococcus acidilactici, Lactobacillus paracasei, Lb. curvatus, Lb. fermentum and Weisssella paramesenteroides. P. acidilactici was the dominant species (47.8%) followed by Lb. paracasei (17.5%). W. paramesenteroides and Lb. fermentum were not detected during the spoilage at ambient temperature while at 4 degrees C W. paramesenteroides and Lb. paracasei have not been detected. For AAB, the species found were Acetobacter pasteurianus sub paradoxus and Acetobacter cerevisiae. These species were common to all samples during spoilage and A. pasteurianus sub paradoxus was the most frequently detected.
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关键词/主题词
Acetic acid bacteria;Lactic acid bacteria;PCR-RFLP;Spoilage;Tchapalo;Yeast;
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