Amperometric sensors and devices for measuring concentration of S-nitrosothiols based on photo-induced decomposition of S-nitrosothiols 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US9702842 B2 2017-07-11 [US9702842]US2014014512 A1 2014-01-16 [US20140014512] / 2017-07-112014-01-16
申请号/申请日
2011US-13264382 / 2011-04-14
发明人
HONG SUNG A;SUNG DA YEON;HAN JUN HEE;SONG JEA HUN;NAM HAKHYUN;CHA GEUN SIG;SHIN JAE HO;
申请人
I SENS;
主分类号
IPC分类号
G01N-027/30
摘要
(US9702842) Provided are an amperometric sensor and device for electrochemically quantifying nitrosothiol (RSNO), which is associated with storage and delivery of nitric oxide (NO) in the human body.  The amperometric sensor for measuring a concentration of RSNO includes an electrode configured to measure an electric current generated by an oxidation reaction of NO, and a means configured to start and stop photo-induced decomposition of RSNO.  Here, the electric current is measured by the oxidation reaction of NO before and after the photo-induced decomposition of RSNO.  The amperometric sensor may measure separate signals of RSNO and NO, which are present in the sample at the same time, using one electrode, thereby preventing an inhibition action caused by NO during the measurement of RSNO.  Also, the amperometric sensor is simple in structure and easy to manufacture and may be applied to manufacture of a small electrode, and thus may be developed as a sensor for in vivo measurements.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2011KR-0028575 2011-03-30 2011KR-0034662 2011-04-14 2011WO-KR02688 2011-04-14
主权利要求
(US9702842) What is claimed is: 1.  An apparatus, comprising: an amperometric sensor for measuring a concentration of nitrosothiols (RSNO), the amperometric sensor comprising:   an electrode located on an optical fiber, the electrode comprising: a conducting wire wound about an outer surface of the optical fiber;   a carbon paste arranged to cover a portion of an end of the optical fiber and the conducting wire; and   an insulating film located over a portion of the carbon paste;    a nitric oxide (NO) selective permeable film located over a portion of the electrode, the electrode configured to measure an electric current generated by an oxidation reaction of nitric oxide (NO), the electrode sized to be insertable into a human body;   a light source communicating with the optical fiber;   a photocatalyst switch that controls emission of a light from the light source; and   wherein the electric current is measured by an electrochemical measurement device to determine an oxidation reaction of NO before and after a light-induced decomposition of RSNO, while the electrode is located in the human body;   wherein the nitric oxide (NO) selective permeable film is formed through a self-assembly reaction in a sol-gel process by mixing a silane monomer used to form a backbone with a perflourinated silane monomer used to give a film lipophilicity is formed on the electrode. 2. The apparatus of claim 1, wherein the electrode is formed on the optical fiber by depositing one material selected from the group consisting of platinum, gold, silver, vanadium, niobium, tantalum, indium, titanium, nickel, molybdenum, iron, copper, cobalt, chromium, bismuth, aluminum, nickel chromium and a combination thereof on an end surface of the optical fiber. 3. The apparatus claim 1, wherein the electrode is formed on the optical fiber by manufacturing one material selected from the group consisting of platinum, gold, silver, vanadium, silicon oxide, niobium, tantalum, indium, titanium, nickel, molybdenum, iron, copper, cobalt, chromium, graphite, bismuth, aluminum, zinc oxide manganese, nickel chromium and a combination thereof in a wire, rod or plate shape and attaching the material in the wire, rod or plate shape to the optical fiber. 4. The apparatus of claim 1, wherein the electrode is formed on the optical fiber by coating a mixed composition, which comprises a paraffinic oil and at least one material selected from the group consisting of gold, carbon, silver, platinum and a mixture of silver and carbon, on an end surface of the optical fiber. 5. The apparatus of claim 1, wherein the electrode is formed by coating a mixed composition, which comprises a paraffinic oil and at least one material selected from the group consisting of gold, carbon, silver, platinum and a mixture of silver and carbon, on an end surface of a cylindrical optical fiber, an operating circuit connection line is mounted on a lateral surface of the optical fiber, and a coating layer from the mixed composition is formed on a lateral surface of the optical fiber comprising the operating circuit connection line. 6. The apparatus of claim 5, wherein a coating of an insulating material is further provided over the coating layer formed on the lateral surface of the optical fiber. 7. The apparatus of claim 1, wherein the optical fiber is in a cylindrical shape having a diameter of 250 mu m to 3 mm, and made of plastic, silica (SiO2) or multicomponent glass. 8. The apparatus of claim 1, wherein the optical fiber is a single mode optical fiber (SMF), a multiple mode optical fiber (MMF), a step index-type (SI) optical fiber or a graded index-type (GI) optical fiber. 9. The apparatus of claim 1, wherein the photocatalyst switch configured to start and stop the light-induced decomposition of RSNO supplies and intercepts light from the light source to a dark room in which the electrode is disposed. 10. The apparatus of claim 1, wherein the light-induced decomposition is caused by light having a maximum wavelength range from an ultraviolet region (320 to 350 nm) to a visible region (550 to 590 nm). 11. The apparatus of claim 1, wherein the RSNO is at least one selected from the group consisting of S-nitrosoalbumin (Alb-NO), S-nitroso-1-cysteine (CysNO), S-nitroso-1-cysteinemethylester (CMESNO), S-nitroso-d,1-penicillamine (PSNO), S-nitroso-N-acetylcysteineamine (ACSNO), S-nitroso-N-acetyl-1-cysteine (NACysNO), S-nitrosocaptopril (SNOCAP), S-nitroso-mercaptoethylamine, S-nitroso-3-mercapto-propanoic acid, S-nitrosohomocysteine (HcysNO), S-nitroso-b,d-thioglucose, S-nitroso-N-acetyl-d, 1-penicillamine (SNAP), S-nitroso-N-acetyl-d, 1-penicillaminyl glycine methyl ester and S-nitroso-1-glutathione (GSNO). 12. The apparatus of claim 1, further comprising: a cell configured to mount the amperometric sensor;   a pump configured to transport a sample into the cell; and   a tubing configured to supply the sample into the cell or discharge the sample from the cell. 13. The apparatus of claim 12, wherein the cell comprises: passages configured to mount a working electrode, an auxiliary electrode and a reference electrode, respectively;   a channel configured to connect the passages and allow the sample to flow in the cell; and   a connection unit connected to a tubing configured to supply the sample from the outside into the cell and discharge the sample from the cell to the outside. 14. The apparatus of claim 12, wherein the device uses a light source selected from the group consisting of a laser, a halogen lamp, a metal halide lamp, a sodium vapor light, a three-wavelength electrodeless lamp, a LED, an incandescent lamp, a fluorescent lamp and a high-frequency lamp.
法律状态
(US9702842) LEGAL DETAILS FOR US2014014512  Actual or expected expiration date=2031-07-03    Legal state=ALIVE    Status=GRANTED     Event publication date=2011-04-14  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US13264382  Application date=2011-04-14  Standardized application number=2011US-13264382     Event publication date=2011-10-13  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: I-SENS, INC., KOREA, REPUBLIC OF ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:HONG, SUNG A SUNG, DA YEON HAN, JUN HEE AND OTHERS SIGNING DATES FROM 20111010 TO 20111011 REEL/FRAME:027059/0326     Event publication date=2013-10-09  Event code=US/APPFILEREC  Event type=Administrative notifications  Event type=OAO  Filing Receipt Action Recorded:  RECD=2013-10-09     Event publication date=2013-12-04  Event code=US/RECEIPTUP  Event type=Administrative notifications  Event type=OAO  Filing Receipt - Updated Action Recorded:  RECD=2013-12-04     Event publication date=2013-12-04  Event code=US/M903  Event type=Examination events  Event type=OAO  Notice of DO/EO Acceptance Mailed Action Recorded:  RECD=2013-12-04     Event publication date=2014-01-16  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2014014512  Publication stage Code=A1  Publication date=2014-01-16  Standardized publication number=US20140014512     Event publication date=2014-01-16  Event code=US/PGPUBN  Event indicator=Pos  Event type=Examination events  Event type=OAO  PG-Pub Issue Notification Action Recorded:  RECD=2014-01-16     Event publication date=2014-02-07  Event code=US/DOCK  Event type=Examination events  Case Docketed to Examiner in GAU Action Recorded:  RECD=2014-02-07     Event publication date=2014-07-10  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection Action Recorded:  RECD=2014-07-10     Event publication date=2014-09-17  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed Action Recorded:  RECD=2014-09-17     Event publication date=2015-04-06  Event code=US/CTFR  Event type=Examination events  Event type=OA  Event type=OAO  Final Rejection Action Recorded:  RECD=2015-04-06     Event publication date=2015-06-22  Event code=US/CTAV  Event type=OAO  Advisory Action (PTOL-303) Action Recorded:  RECD=2015-06-22     Event publication date=2015-07-08  Event code=US/RCEX  Event type=Examination events  Event type=RCE  Event type=OAI  Request for Continued Examination (RCE) Action Recorded:  RECD=2015-07-08     Event publication date=2015-07-09  Event code=US/DRCECAP  Event type=RCE  Event type=OAO  Disposal for a RCE / CPA / R129 Action Recorded:  RECD=2015-07-09     Event publication date=2016-06-12  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection Action Recorded:  RECD=2016-06-12     Event publication date=2017-03-15  Event code=US/EXAC  Event type=Examination events  Event type=OAO  Examiner's Amendment Communication Action Recorded:  RECD=2017-03-15     Event publication date=2017-03-17  Event code=US/NOAM  Event type=Examination events  Event type=OAO  Mail Notice of Allowance Action Recorded:  RECD=2017-03-17     Event publication date=2017-04-11  Event code=US/APRDY  Event type=Examination events  Application Is Considered Ready for Issue Action Recorded:  RECD=2017-04-11     Event publication date=2017-06-07  Event code=US/ANA  Event type=Corrections  Event type=Examination events  Event type=OA  Amendment after Notice of Allowance (Rule 312) Action Recorded:  RECD=2017-06-07     Event publication date=2017-06-08  Event code=US/N271  Event type=Examination events  Event type=OAO  Response to Amendment under Rule 312 Action Recorded:  RECD=2017-06-08     Event publication date=2017-06-21  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: KESSEL MARIS    Event publication date=2017-06-21  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1759     Event publication date=2017-06-21  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: 15069-300US Customer Nbr: 60468    Event publication date=2017-06-21  Event code=US/ENT  Event type=Administrative notifications  Business Entity Status: UNDISCOUNTED    Event publication date=2017-06-21  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=2017-07-11  Event code=US/B2  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as second publication  Publication country=US  Publication number=US09702842  Publication stage Code=B2  Publication date=2017-07-11  Standardized publication number=US9702842     Event publication date=2017-07-11  Event code=US/354  Event indicator=Pos  Event type=Extension of term of duration of protection  Patent term extended under  35 U.S.C 154(b) until/for Delays (A,B,C): 0  Overlap Delays: 0  Non Overlap Delays: 220   PTO Office Delays: 0  Applicant Delays: 140  Adjustment total:  Number of days of extension=80
专利类型码
B2A1
国别省市代码
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