Mixing and matching tc proteins for pest control 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
CA2514041 A1 2004-08-12 [CA2514041] / 2004-08-12
申请号/申请日
2004CA-2514041 / 2004-01-07
发明人
SCHLEPER AMANDA D;BEVAN SCOTT A;MERLO DONALD J;HEY TIMOTHY D;APEL-BIRKHOLD PATRICIA C;ZHU BAOLONG;MEADE THOMAS;MITCHELL JON C;LI ZE SHENG;NI WEITING;BINTRIM SCOTT B;
申请人
DOW AGROSCIENCES;
主分类号
IPC分类号
A01N-037/46A01N-063/02C07K-014/195C07K-014/24C12N-015/82
摘要
(CA2514041) The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other.  In particularly preferred embodiments of the subject invention, the toxicity of a "stand-alone" TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein "potentiators" derived from a source organism of a different genus from which the toxin was derived.  As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have.  Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin.  This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests.  Certain preferred combinations of heterologous TC proteins are also disclosed herein.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2003US-60441723 2003-01-21 2004WO-US00394 2004-01-07
主权利要求
(CA2514041)  Claims 1. A method of controlling or inhibiting an insect which comprises contacting said insect with effective amounts of a Protein A, a Protein B, and a Protein C, wherein: (i) each of said Proteins A, B, and C is encoded by a naturally occurring gene or has an amino acid sequence that differs from the product encoded by a naturally occurring gene only by truncation or by conservative amino acid changes;  (ii) said Protein A is a 230-290 kDa toxin complex insect toxin that is derived from a first taxonomic species, has stand alone insecticidal. activity, and has an amino acid sequence at least 40% identical to a sequence selected from the group consisting of SEQ ID NO:14 (XptAlXwi), SEQ ID NO:34 (XptA2 Xwi), SEQ ID NO:21 (TcdA), SEQ ID NO:62 (TcdA2), SEQ ID NO:63 (TcdA4), and SEQ ID NO:59 (TcbA);  (iii) said Protein B is a 130-180 kDa toxin complex potentiator having an amino acid sequence at least 40% identical to a sequence selected from the group consisting of SEQ ID NO:22 (TcdBl), SEQ ID NO:45 (TcdB2), SEQ ID NO:56 (TcaC), SEQ ID NO:18 (XptClx, i), -SEQ ID NO:49 (XptB1x0, SEQ ILA NO: 40 (PptB1(orf5)), and SEQ ID NO:60 (SepB);  (iv) said Protein C is a 90-120 kDa toxin complex potentiator having an amino acid sequence at least 35% identical to a sequence selected from the group consisting of SEQ ID NO:25 (TccCl), SEQ ID NO:58 (TccC2), SEQ ID NO:47 (TccC3), SEQ ID NO:64 (TccC4), SEQ ID NO:57(TecC5), SEQ ID NO:16 (XptBlxwi), SEQ ID NO:51 (XptClxb), SEQ IDNO:43 (PptCl (orf 6 long)), SEQ ID NO:42 (PptCl (orf 6 short)), and SEQ ID NO:61 (SepC);  (v) at least one of said Protein B and said Protein C is derived from a second taxonomic species that is different from said first taxonomic species;  (vi) if said Protein B is derived from said second taxonomic species, then the amino acid sequence of said Protein B is less than 75% identical to the amino acid sequence of any protein known to be produced by said first taxonomic species;  and (vii) if said Protein C is derived from said second taxonomic species, then the amino acid sequence of said Protein C is less than 75% identical to the amino acid sequence of any protein known to be produced by said first taxonomic species;  wherein a given protein is considered to be derived from a particular species if either the genome of that species contains a gene that encodes the protein or the given protein was designed by truncating or making conservative amino acid changes in the amino acid sequence of a protein encoded by a gene contained in the genome of that species. 2. The method of claim 1 wherein said first and second taxonomic species are from different genera. 3. The method of claim 2 wherein said Protein A is derived from a Photorhabdus species and at least one of said Protein B and Protein C is derived from a Xenorhabdus, Paenibacillus, Serratia or Pseudoinonas species. 4. The method of claim 2 wherein said Protein A is derived from a Xenorhabdus species and at least one of said Protein B and Protein C is derived from a Photorhabdus, Paenibacillus, Serratia or.  Pseudomonas species. 5. Tht method of claim 2 wherein at least one of said Protein A, Protein B, and Protein. C is derived from a Xenorhabdus species and at least one of said Protein A, Protein B, and Protein C is derived from a Photorhabdus species. 6. The method of claim 2 wherein at least one of said Protein A, Protein B, and Protein C is derived from a Photorhabdus species and at least one of said Protein A, Protein B, and Protein C is derived from a Xenorhabdus species. 7. The method of claim 1 wherein: (i) Protein A is SEQ ID NO: 34(XptA2x w 1) or SEQ ID NO:21 (TcdA), (ii) Protein B is SEQ ID NO:45 (TcdB2), SEQ ID NO:40 (Pp - LB 1 1529), or SEQ ID NC):49 (XptBlxb), and (iii) Protein C is SEQ ID NO:47 (TccC3), SEQ ID NO:42 (PptCl 1529-short), SEQ ID NO:43 (PptC11529-long),or SEQ ID NO:51 (XptClxb). 8. The method of claim 5 wherein said Protein A has at least 40% identity with a protein selected from the group consisting of SEQ ID NO:14 (XptAlx w i) and SEQ ID NO:34 (XptA2x w 1). 9. The method of claim 6 wherein said Protein A has at least 40% identity with a protein selected from the group consisting of SEQ ID NO:21 (TcdA) and SEQ ID NO:59 (TcbA). 10. The method of claim 8 wherein Protein B has at least 40% identity with an amino acid sequence selected from the group consisting of SEQ ID NO:22, SEQ ID NO:40, SEQ ID NO:45, and.  SEQ ID NO:49, or Protein C has at least 35% identity with an amino acid sequence selected from the group consisting of SEQ ID NO:25, SEQ ID NO:42, SEQ ID NO:47, and SEQ ID NO:51. 11. The method of claim 9 wherein Protein B has at least 40% identity with an amino acid sequence selected from the group consisting of SEQ ID NO:18 and SEQ ID NO:40;  or.  Protein C has at least 35% identity with an amino acid sequence selected from the group consisting of SEQ. ID NO:16 (XptB i N O and SEQ ID NO:42. 12. The method of claim 5 wherein said Protein A is encoded by a polynucleotide that hybridizes under stringent conditions with a probe that is the full complement of a nucleic acid sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO:-14 (XptAlwi) and SEQ ID NO:20 (XptA2wi). 13. The method of claim 6 wherein said Protein A is encoded by a polynucleotide that hybridizes under stringent conditions with a probe that is the full complement of a nucleic acid sequence that encodes the amino acid sequence shown in SEQ ID NO:21 (TcdA). 14. The method of claim 8 wherein Protein B is encoded by a polynucleotide that hybridizes under stringent conditions with a probe that is the full complement of a nucleic acid sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO:22, SEQ ID NO:40 SEQ ID NO:45, and SEQ ID NO:49, or said Protein C is encoded by a polynucleotide that hybridizes under stringent conditions with a probe that is the full complement of a nucleic acid sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO:25, SEQ ID NO:42, SEQ ID NO:47, and SEQ ID NO:51. 15. The method of claim 9 wherein said Protein B is encoded by a polynucleotide that hybridizes under stringent conditions with a probe that is the full complement of a nucleic acid sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO:18 and SEQ ID NO:40, or said Protein C is encoded by a polynucleotide that hybridizes under stringent conditions with a probe that is the full complement of a nucleic acid sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO:16 (XptBl w,), and SEQ ID NO:42. 16. A transgenic plant or plant cell that produces a Protein A, a Protein B, and a Protein C, wherein (i) each of said Proteins A, B, and C is encoded by a naturally occurring gene or has an amino acid sequence that differs from the product encoded by a naturally occurring gene only by truncation or by conservative amino acid changes;  (ii) said Protein A is a 230-290 kDa toxin complex insect toxin that is derived from a first taxonomic species, has stand alone insecticidal activity, and has an amino acid sequence at least 40% identical to a sequence selected from the group consisting of SEQ ID NO:14 (XptAlxwi), SEQ ID NO:34 (XptA2x w i), SEQ ID NO:21. (TcdA), SEQ ID NO:62 (TcdA2), SEQ ID NO:63 (TcdA4), and SEQ ID NO:59 (TcbA);  (iii) said Protein B is a 130-180 kDa toxin complex potentiator having an amino acid sequence at least 40% identical to a sequence selected from the group consisting of SEQ ID NO:22 (TcdB1), SEQ ID NO:45 (TcdB2), SEQ ID NO:56 (TcaC), SEQ ID NO:18 (XptC1 SEQ ID NO:49 (XptBlxb), SEQ ID NO:40 (PptB1(orf5)), and SEQ ID NO:60 (SepB);  (iv) said Protein C is a 90-120 kDa toxin complex potentiator having an amino acid sequence at least 35% identical to a sequence selected from the group consisting of SEQ ID NO:25 (TccCl), SEQ ID NO:58 (TccC2), SEQ ID NO:47 (TccC3), SEQ ID NO:64 (TccC4), SEQ ID NO:57 (TecC5), SEQ ID NO:16 (XptBlx,), SEQ ID NO:51 (XptClxb), SEQ ID NO:43 (PptC1 (orf 6 long)), SEQ ID NO:42 (PptC1 (orf 6 short)), and SEQ ID NO:61 (SepC);  (v) at least one of said Protein B and said Protein C is derived from a second taxonomic species that is different from said first taxonomic species;  (vi) if said Protein B is derived from said second taxonomic species, then the amino acid sequence of said Protein B is less than 75% identical to the amino acid sequence of any protein known to be produced by said first taxonomic species;  (vii) if said Protein C is derived from said second taxonomic species, then the amino acid sequence of said Protein C is less than 75% identical to the amino acid sequence of any protein known to be produced by said first taxonomic species;  wherein a given protein is considered to be derived from a particular species if either the genome of that species contains a gene that encodes the protein or the given protein was designed by truncating or making conservative amino acid changes in the amino acid sequence of a protein encoded by a gene contained in the genome of that species. 17. A transgenic plant or plant cell of claim 16 wherein: (i) Protein A is SEQ ID NO:34 (XptA2x, i) or SEQ ID NO:21 (TcdA), (ii) Protein B is SEQ ID NO:45 (TcdB2), SEQ ID NO:40 (PptB11:529), or SEQ ID N0:49 (XptB 1X 1,), and (iii) Protein C is SEQ ID NO:47 (TccC3);  SEQ ID NO:42 (PptC11529-short), SEQ ID NO:43 (PptC11529-long), or SEQ ID NO:51 (XptClxb). 18. A method of claim 1 wherein said insect is brought into "contact with a second Protein A that is derived from a taxonomic species different from said first taxonomic species. 19. A method of claim 18 wherein said insect is brought into contact with a first Protein A selected from SEQ ID NO:14 (XptAlx, i) and SEQ ID NO:34 (XptA2x,; ) and a second Protein A selected from SEQ ID NO:21 (TcdA) and SEQ ID NO:59 (TcbA). 20. A method of claim 18 wherein said first Protein A is SEQ ID NO:14 (XptAlx, i), said second protein A is SEQ ID NO:21 (TcdA), said protein B is SEQ ID NO:45 (TcdB2) and said protein C is SEQ ID NO: 47 (TccC3).
法律状态
(CA2514041) LEGAL DETAILS FOR CA2514041  Actual or expected expiration date=2012-08-01    Legal state=DEAD    Status=LAPSED     Event publication date=2004-01-07  Event code=CA/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=CA CA2514041  Application date=2004-01-07  Standardized application number=2004CA-2514041     Event publication date=2004-08-12  Event code=CA/A1  Event type=Examination events  Application laid open  Publication country=CA  Publication number=CA2514041  Publication stage Code=A1  Publication date=2004-08-12  Standardized publication number=CA2514041     Event publication date=2008-11-05  Event code=CA/EEER  Event indicator=Pos  Event type=Examination events  Examination request    Event publication date=2012-08-01  Event code=CA/FZDE  Event indicator=Neg  Event type=Event indicating Not In Force  Dead
专利类型码
A1
国别省市代码
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