一种​采用​苹果​木​栽培​牛樟​芝​子实​体​的​方法​及其​培养基

源语言标题
(CN105906415) 一种​采用​苹果​木​栽培​牛樟​芝​子实​体​的​方法​及其​培养基
公开号/公开日
CN105906415 A 2016-08-31 [CN105906415] / 2016-08-31
申请号/申请日
2016CN-0238278 / 2016-04-18
发明人
CHENG XIANHAO; FENG LUYAO; LIU JING; SUN LEI; HAO BAOJIAN
申请人
LUDONG UNIVERSITY
主分类号
IPC分类号
A01G-001/04;C05G-003/00;C12N-001/14
摘要
(CN105906415)
本​发明​公开​了​种​采用​苹果​木​栽培​牛樟​芝​子实​体​的​方法,其​包括​在​适合​牛樟​芝​生长​的​条件下,在​液体​种子​培养基​或​固体​种子​培养基​中​培养​牛樟​芝,获得​液体​或​固体​牛樟​芝​种子;采用​苹果​木桩​浸泡​清洗,装入​聚丙烯​菌​袋​封口​扎紧​灭菌;在​无菌​环境​下​打开​装有​苹果​木​的​菌​袋,接入​固体​或​液体​牛樟​芝​种子,封口​扎紧;将​接种​完​的​菌​袋​进行​暗​培养,完成​营养​生长​管理;脱​袋​培养,牛樟​芝​子实​体长​到​2cm​以上​大小,可以​进行​采收,完成​生殖​生长​管理。其​所用​胡萝卜​培养基​原料​包括:胡萝卜​沸水​煮​后​过滤​的​滤液、葡萄糖、琼脂,余量​水;其​所用​液体​种子​培养基​原料​包括:固形物​含量​为​5%(W/V)的​麦芽汁;栽培​培养基​为​苹果​木。本​发明​操作​简单,培养​周期​相对​较短,原料​来源​广泛,成本​低廉。
地址
代理人
代理机构
优先权号
2016CN-0238278 2016-04-18
主权利要求
(CN105906415)
1. A method for growing medium of antrodia child entity by apple wood, characterized in media feed comprises: carrot 20% (W/V), 2% glucose (W/V), 1.5% agar (W/V), balance water, pH natural; wherein carrots were cut into 1 cm3 size, boiling water 20min, the filtrate was collected by filtration through use. 
2. A method for using a liquid seed culture growing apple wood antrodia child entity, characterized in media feed comprises: a solid content of 5% (W/V) of the wort, natural pH. 
3. One use of the antrodia child entity apple wood-growing method, characterized in comprising the steps of: 1), species: camphorata species, available from Guangzhou Culture Collection, stored in the PDA slant culture; 2), in a suitable growth conditions camphorata, in a liquid seed culture or solid seed culture medium bovine antrodia, liquid or solid bovine antrodia seed obtained; wherein liquid with a liquid seed culture with 500 ml bovine antrodia a hydroponic seed culture Erlenmeyer flask, 150 ml/500 ml bottle loading; access activated camphorata species, a rotary shaker, speed 120 rpm; temperature 28 Ć, dark culture 10-12 days; antrodia a hydroponic seed culture medium where solid cattle using carrots, after inoculation temperature ramp camphorata species 28 Ć, 20 day culture dark; 3), camphorata culture medium was prepared: about 30 cm of the growth of the aging of the apple muju peg, the peg is fully immersed in water the saw good soaking 7-10 days, cleaning after removal, in the loaded polypropylene jundai, sealed with absorbent cotton, cotton string for tightening; 121 Ć, sterilization 120min; 4), inoculated: a clean workstation, the liquid fermentative species shaking well, alcohol burner flame of the culture base port opened sterile area, burning mouth; subject jundai after cooling, opening jundai, antrodia seed access solid or liquid cattle, with absorbent cotton closure, with a cotton rope tightening; wherein each bag access 2-3 blocks 150 ml liquid per bag access seed or solid bovine antrodia seed antrodia cattle, antrodia seed from bovine to assure that liquid flowing down the sides of the apple bolts; 5), vegetative growth management: that has been already placed on the inoculated jundai 15 Ć-30 Ć Ć culture chamber performs the dark for 30-60 days, hyphae spread surface covered at bolts, growth and deeper into the wood interior; 6), reproductive growth management: off the bag, is placed in a humidity of 80-95%, temperature 15-25 Ć Ć culture chamber are cultured, culture without illumination; off the bag after 30 days after culturing, the fruiting body length to 2 cm or more camphorata size, it is possible to perform recovery.
法律状态
(CN105906415)
LEGAL DETAILS FOR CN105906415

Actual or expected expiration date=2036-04-18   
Legal state=ALIVE   
Status=PENDING 



Event publication date=2016-04-18 
Event code=CN/APP 
Event indicator=Pos 
Event type=Examination events 
Application details

Application country=CN CN201610238278 
Application date=2016-04-18 
Standardized application number=2016CN-0238278 



Event publication date=2016-08-31 
Event code=CN/A 
Event type=Examination events 
Published application

Publication country=CN 
Publication number=CN105906415 
Publication stage Code=A 
Publication date=2016-08-31 
Standardized publication number=CN105906415 



Event publication date=2016-08-31 
Event code=CN/C06 
Event indicator=Pos 
Event type=Examination events 
Publication



Event publication date=2016-09-28 
Event code=CN/C10 
Event type=Examination events 
Request of examination as to substance
专利类型码
A
国别省市代码
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