Pseudorabies virus (PRV) is considered as an infectious agent with a wide of host range, causing considerable) economic losses in animal husbandry. Although the commercial vaccine against PRV plays an critical role control of this disease in swine industry, the potential risk of commercial vaccines against PRV for other host unclear. Here, we report that the commercial vaccine against PRV is a hidden health risk for dogs. We found that different attenuated PRV strains in commercial vaccines possess different tissue tropism, and that the attenuate PRV strains are lethal to dogs, and that the attenuated PRV strain possesses the ability to spread horizontally among the dogs. Collectively, our findings provide clues that the commercial vaccine against PRV is a hidden risk for dogs, even for the owner of pet dogs to take seriously.