Method for changing the development pattern, increasing the growth and accumulation of starch, changing the structure of starch and increasing the resistance to hydric stress in plants 机翻标题: 暂无翻译,请尝试点击翻译按钮。

源语言标题
(WO2011135121) Procedimiento para alterar el patron de desarrollo, aumentar el crecimiento y la acumulación de almidón, alterar la estructura del almidón y aumentar la resistencia al estrés hídrico en plantas
公开号/公开日
WO2011135121 A2 2011-11-03 [WO2011135121] WO2011135121 A3 2012-03-08 [WO2011135121] / 2011-11-03 2012-03-08
申请号/申请日
2011WO-ES00125 / 2011-04-15
发明人
BAROJA FERNANDEZ MIREN EDURNE; LI JUN; POZUETA ROMERO JAVIER; EZQUER GARIN IGNACIO; ABDELLATIF BAHAJI; MUNOZ PEREZ FRANCISCO JOSE; OVECKA MIROSLAV
申请人
IDEN BIOTECHNOLOGY
主分类号
IPC分类号
A01N-063/02
摘要
(WO2011135121)
The invention relates to a method for changing the development pattern, increasing the growth and accumulation of starch, changing the structure of starch and increasing the resistance to hydric stress in plants. 
The method involves cultivating plants in an atmosphere containing volatile elements emitted by a microorganism, without there being any physical contact between the microorganism and the plant. 
The method is based on the discovery that the volatile elements emitted by Gram-positive or Gram-negative Gram bacteria, yeast and microscopic fungi stimulate an increase in the growth of plants in general, with an increase in the length, the number of leaves and/or the number of branches of the plant, an increase in the accumulated starch and structural change to said biopolymer, and modification of the development pattern, with an increase in floral buds. 
An increased resistance to hydric stress can also be observed, in addition to an increase in starch in leaves separated from whole plants.
机翻摘要
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地址
代理人
代理机构
优先权号
2010ES-0000499 2010-04-19 2010ES-0001068 2010-08-13 2011ES-0000405 2011-04-07
主权利要求
(WO2011135121)
1. A method for increasing the growth of a plant and / or alter their development pattern characterized in that the plant is grown in the presence of a culture of a microorganism which produces volatile compounds without contact between the ground and the microorganism or in presence of the volatiles emitted by the microorganism, wherein the microorganism is other than Bacillus subtilis isolates GB03 and Bacillus amyloliquefaciens IN937.
2.
2. Method according to claim 1, wherein the microorganism is a bacterium, a yeast or multicellular microscopic fungus.
3.
3. Method according to claim 2, wherein the microorganism is a bacterium belonging to a genus other than Bacillus or Paenibacillus.
4.
4. Method according to any one of the preceding claims, in which the growth of the microorganism occurs in a medium lacking organic compound having amino groups
5.
5. Method according to claim 4, in which the growth of the microorganism occurs in a medium lacking amino acids and / or proteins.
6.
6. The method of claim 4 or 5, in which the growth of the microorganism occurs in a minimal medium supplemented with an organic compound as a carbon source.
7.
7. Method according to claim 6, wherein the microorganism is a bacterium from a genus of the group of Bacillus, Escherichia, Salmonella, Agrobacterium or Pseudomonas.
8.
8. Method according to claim 6, wherein the microorganism is a fungus belonging to the genus Penicillium and Alternaria.
9.
9. Method according to any one of the preceding claims, wherein the plant is an angiosperm, monocot or dicot.
10.
10. Method according to claim 9, wherein the plant is selected from the group of potato plants, maize plants, pepper plants, snuff plants, or plants of the species Arabidopsis thaliana.1.
1. Method according to claim 10, wherein the plant is a corn plant, a snuff or plant species Arabidopsis thaliana grown in the presence of a fungus belonging to the genus Alternaria or Penicillium is allowed to grow in minimal medium supplemented with a source of organic carbon, without contact between the plant and the organism.
12.
12. Method according to any one of the preceding claims, wherein increased growth is reflected in increased length of the plant, increased leaf size, increased stem thickness and / or increasing the size of the roots.
13.
13. Method according to any one of the preceding claims, wherein the altered pattern of growth is manifested in increased number of leaves, increased number of branches and / or the number of flowers and seeds of plants angiosperms, in the induction of flowering, or combinations thereof.
14.
14. Method according to any one of the preceding claims, in which the plant is grown in an atmosphere in which the volatiles emitted by a microorganism are present.
15.
15. The method of claim 14, wherein the volatile present in the atmosphere by evaporation from a solution which contains them and which is in the place of cultivation of the plant.
16.
16. The method of claim 15, wherein the volatile content in the solution are mixed volatiles present in the culture medium of the microorganism, after filtering it.
17.
17. The method of claim 15 or 16, wherein the solution from which the water is evaporated volatile irrigation plant.
18.
18. The method of claim 14, wherein the volatile compounds are injected into the atmosphere of cultivation.
19.
19. The method of claim 18, wherein the plant is grown in a greenhouse.
20.
20. The method of claim 14, wherein the volatile compounds are administered to the atmosphere via the plant crop spraying or spraying.
21.
21. Method according to any one of claims 14 to 20, in which volatile compounds are administered to the culture atmosphere with a fertilizer, pesticide, or mixtures thereof.
22.
22. A method for increasing the growth of a plant and / or alter their development pattern characterized in that the plant is grown in an atmosphere in which at least one volatile compound is selected from propionic acid, acetic acid, acetaldehyde is present, acid formic acid and butyric acid.
23.
23. The method of claim 22, wherein the plant is grown in an atmosphere in which at least formic acid is present.
24.
24. The method of claim 22 or 23, in which the volatile compound is present in the atmosphere by evaporation from a solution containing it which is in the place of cultivation of the plant.
25.
25. The method of claim 24, wherein the solution is water irrigation plant.
26.
26. Method according to claim 22 or 23, in which the volatile compound is supplied to the culture atmosphere from outside the site of the plant cultivation.
27.
27. The method of claim 26, wherein the volatile compound is blown culture atmosphere.
28.
28. The method of claim 22 or 23, the volatile compound is administered to the atmosphere via the plant crop spraying or spraying.
29.
29. Method according to any one of claims 24 to 28, in which the volatile compound is administered to the growing atmosphere together with a fertilizer, pesticide, or mixtures thereof.
30.
30. Method according to any one of claims 22 to 29, in which the increase in growth is manifested in increased plant length and / or increasing the size of the leaves, stems and roots.
31.
31. Method according to any one of claims 22 to 29, in which the altered pattern of growth is manifested in increased number of leaves, increased number of branches and / or the number of flowers and seeds of plants angiosperms, in inducing Bloom, or combinations thereof
32.
32. A method for increasing the amount of starch accumulated in a plant, characterized in that the plant is grown in the presence of a culture of a microorganism which produces volatile compounds without contact between the ground and the microorganism or in the presence of volatile issued by the microorganism.
33.
33. The method of claim 32, wherein additionally there is increased size of the starch granules and a modified starch is obtained, wherein the relative amylose content and degree of polymerization of amylopectin chains are less than starch produced by control plants grown in the absence of microbial volatile.
34.
34. The method of claim 32 or 33, wherein the microorganism is a bacterium, a yeast or multicellular microscopic fungus.
35.
35. The method of claim 34, wherein the microorganism is a yeast.
36.
36. The method of claim 35, wherein the yeast belongs to the species Saccharomyces cerevisiae.
37.
37. Method according to any one of claims 32 to 36, in which the growth of the microorganism occurs in a medium lacking organic compound having amino groups.
38.
38. The method of claim 37, wherein the growth of the microorganism occurs in a medium lacking amino acids and / or proteins.
39.
39. The method of claim 37 or 38, in which the growth of the microorganism occurs in a minimal medium supplemented with a source of organic carbon.
40.
40. The method of claim 39, wherein the microorganism is a bacterium from a genus of the group of Salmonella, Agrobacterium, Bacillus, Pseudomonas or Escherichia.
41.
41. The method of claim 40, wherein the microorganism is selected from the group of Bacillus. subtilis 168, Salmonella enterica (LT2), Escherichia. coli (BW25113), Agrobacterium tumefaciens EHA105, GV2260 Agrobacterium tumefaciens, Pseudomonas syringae 1448A9, 49a/90 Pseudomonas syringae, Pseudomonas syringae PK2.
42.
42. The method of claim 37, wherein the microorganism is a fungus belonging to the genus Penicillium and Alternaria.
43.
43. Method according to any one of claims 32 to 42, wherein the plant is an angiosperm, monocot or dicot.
44.
44. The method of claim 43, wherein the plant is selected from the group of potato plants, maize plants, plants snuff, barley plants, green pepper plants or plants of the species Arabidopsis thaliana.
45.
45. The method of claim 44, wherein the plant is a potato plant, a corn plant, a pepper plant, snuff plant or plant species Arabidopsis thaliana grown in the presence of a fungus belonging to the genus Alternaria or Penicillium is allowed to grow in minimal medium supplemented with a source of organic carbon, without any physical contact between the plant and the organism.
46.
46. The method of claim 45, wherein the medium is supplemented with sucrose.
47.
47. The method of claim 46, wherein the plant is a potato or a corn plant.
48.
48. Method according to any one of claims 32 to 47, wherein the plant is cultivated in vitro or in soil.
49.
49. A method for increasing the amount of starch accumulated in a plant, characterized in that the plant is grown in an atmosphere in which at least one volatile compound is selected from propionic acid, acetic acid, acetaldehyde, formic acid and butyric acid are present.
50.
50. The method of claim 49, wherein the volatile compound is present in the atmosphere by evaporation from a solution containing it which is in the place of cultivation of the plant.
51.
51. The method of claim 50, wherein the solution is water irrigation plant.
52.
52. The method of claim 49, wherein the volatile compound is supplied to the culture atmosphere from outside the site of the plant cultivation.
53.
53. The method of claim 52, wherein the volatile compound is blown culture atmosphere.
54.
54. The method of claim 49, the volatile compound is administered to the atmosphere via the plant crop spraying or spraying.
55.
55. Method according to any one of claims 49 to 54, in which the volatile compound is administered to the growing atmosphere together with a fertilizer, pesticide, or mixtures thereof.
56.
56. A method for obtaining starch from separate sheets of the whole plant, which comprises a step in which the leaves are maintained in the presence of a culture of a microorganism which produces volatile compounds, without any physical contact between the ground and microorganism.
57.
57. The method of claim 56, wherein the microorganism is a fungus of the genus Alternaria
58.
58. The method of claim 56 or 57, wherein the leaves are leaves of a potato plant.
59.
59. Method according to any one of claims 56 to 58, in which the sheets are kept at least 2 days in the presence of the microorganism culture, contact volatiles emitted by the microorganism.
60.
60. A method for increasing the production of starch in a plant, characterized in that the plant is a transgenic plant in which it is present at least a transgene whose expression results in a product selected from the group of: a protease inhibitor of plant, the Branching starch enzyme inhibitor, an acid invertase, antisense RNA directed against the cysteine synthase A antisense RNA directed against nitrite reductase, an antisense RNA directed against glyceraldehyde-3-phosphate dehydrogenase plastid or antisense RNA directed against glucose-6-phosphate dehydrogenase plastid, or combinations thereof.
61.
61. The method of claim 60, wherein the transgenic plant expresses at least one transgene is selected from the group of plant protease inhibitor, an antisense RNA directed against the cysteine synthase A antisense RNA directed against nitrite reductase, or combinations thereof.
62.
62. The method of claim 61, wherein the transgene is the protease inhibitor whose coding sequence is represented by SEQ ID NO: 67 or wherein the antisense RNA expressed from the transgene is directed against cysteine synthase whose coding sequence is represented by SEQ ID NO: 71 or with nitrite reductase whose coding sequence is represented by SEQ ID NO: 69.
63.
63. The method of claim 61 or 62, characterized in that the method includes a step in which a transgene is introduced into the plant by a plasmid comprising at least one sequence selected from those represented by SEQ ID NO: 67, SEQ ID NO: 69 and SEQ ID NO: 71.
64.
64. Method according, to claim 63, wherein the antisense to the coding sequence represented by SEQ ID NO: 69 or SEQ ID NO: 71 is operably linked to the constitutive promoter 35S of cauliflower mosaic virus (CaMV).
65.
65. The method of claim 63, wherein the coding sequence represented by SEQ ID NO: 67 is operably linked to the constitutive promoter 35S of cauliflower mosaic virus (CaMV).
66.
66. Method according to one of claims 63, 64 or 65, wherein the plasmid comprises at least one selectable marker.
67.
67. The method of claim 66, wherein the selectable marker is a gene for resistance to an antibiotic.
68.
68. The method of claim 66, wherein the plasmid comprises at least two selectable markers.
69.
69. The method of claim 68, wherein the selectable marker is a gene conferring resistance to kanacimina and a gene conferring hygromycin resistance.
70.
70. Method according to any one of claims 63 to 69, wherein the plasmid comprises sequences from Agrobacterium tumefaciens Tnos.
71.
71. Method according to any one of claims 63 to 70, wherein the plasmid comprises at least one coding sequence selected from those represented by SEQ ID NO: 67, the antisense sequence represented by SEQ ID NO: 69 or the sequence antisense represented by SEQ ID NO: 71, operably linked to the CaMV 35S promoter, and further comprising sequences of Agrobacterium tumefaciens Tnos a kanamycin resistance gene and hygromycin resistance gene.
72.
72. Method according to any one of claims 61 to 71, wherein the transgenic plant further expresses at least one transgene expressing starch branching enzyme inhibitor, an acid invertase, antisense RNA directed against glyceraldehyde-3-phosphate dehydrogenase plastidial or antisense RNA directed against glucose-6-phosphate dehydrogenase plastid.
73.
73. Method according to any one of claims 61 to 72, wherein the plant also has at least one transgene results in the ectopic expression of the sucrose synthase (SuSy) or transporter expression of glucose-6-phosphate.
74.
74. A method for increasing biomass of a plant characterized in that the plant is grown in an atmosphere in which the volatile compounds emitted by a microorganism that has been grown in a different place from the plant cultivation place are present.
75.
75. A method for increasing the amount of chlorophyll accumulated by a plant, characterized in that the plant is grown in the presence of a culture of a microorganism which produces volatile compounds without contact between the ground and the microorganism or in the presence of volatile issued by the microorganism
76.
76. The method of claim 75, wherein the plant is grown in the presence of a culture of a microorganism producing volatiles.
77.
77. The method of claim 76, wherein the growth of the microorganism occurs in a medium lacking organic compounds which present amino groups.
78.
78. The method of claim 77, wherein the growth of the microorganism occurs in a medium lacking amino acids and / or proteins.
79.
79. The method of claim 78, wherein the growth of the microorganism occurs in a minimal medium supplemented with an organic compound as a carbon source.
80.
80. Method according to any one of claims 76 to 79, wherein the microorganism used is a fungus belonging to the genus Penicillium and Alternaria.
81.
81. The method of claim 80, wherein the growth of the fungus occurs in a minimal medium supplemented with an organic compound as a carbon source.
82.
82. Method according to any one of claims 75 to 81, wherein the plant is an angiosperm, a monocot or a dicot.
83.
83. The method of claim 82, wherein the plant is selected from the group of corn plants and pepper plants.
84.
84. The method of claim 83, wherein the plant is a maize plant or a pepper plant, which is grown in the presence of a fungus belonging to the genus Alternaria or Penicillium is allowed to grow in minimal medium supplemented with a source of organic carbon, without contact between the plant and the organism.
85.
85. A method of increasing a plant's resistance to water stress in which the plant is grown in the presence of a culture of a microorganism which produces volatile compounds without contact between the ground and the microorganism or in the presence of the volatiles emitted by the microorganism.
86.
86. A method for increasing the amount of protein associated to the starch in a plant, characterized in that the plant is grown in the presence of a culture of a microorganism which produces volatile compounds without contact between the ground and the microorganism or in the presence of the volatiles emitted by the microorganism.
87.
87. The method of claim 83 wherein the microorganism is a bacterium, a yeast or multicellular microscopic fungus.
88.
88. Method according to any one of claims 86 or 87, in which the growth of the microorganism occurs in a medium lacking organic compound having amino groups.
89.
89. The method of claim 88, wherein the growth of the microorganism occurs in a medium lacking amino acids and / or proteins
90.
90. The method of claim 88 or 89, in which the growth of the microorganism occurs in a minimal medium supplemented with a source of organic carbon.
91.
91. The method of claim 90, wherein the microorganism is a fungus belonging to the genus Penicillium and Alternaria.
92.
92. Method according to any one of claims 86 to 91, wherein the plant is an angiosperm, monocot or dicot.
93.
93. The method of claim 92, wherein the plant is selected from the group of potato plants, maize plants, plants snuff, barley plants or plants of the species Arabidopsis thaliana.
94.
94. The method of claim 93, wherein the plant is a potato plant, a corn plant, a plant of snuff or plant species Arabidopsis thaliana grown in the presence of a fungus belonging to the genus Alternaria or Penicillium which allowed to grow in minimal medium supplemented with a source of organic carbon, without any physical contact between the plant and the organism.
95.
95. Method according to any one of claims 86 to 94, wherein the plant is a genetically modified plant expressing a fusion protein in which the amino terminus is amino acid sequence of a protein that is associated with the starch granule.
96.
96. The method of claim 95, wherein the amino acid sequence corresponding to a protein that is associated with the starch granule is bound to the amino acid sequence corresponding to the carboxyl terminus of the fusion protein using an amino acid sequence comprising the recognition sequence for a protease.
97.
97. The method of claim 95 or 96, wherein the protein that is associated with the starch granule is starch synthase which binds the granule (GBSS).
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