HIV-1 envelope glycoprotein 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2014212458 A1 2014-07-31 [US20140212458]US9738688 B2 2017-08-22 [US9738688] / 2014-07-312017-08-22
申请号/申请日
2013US-14072209 / 2013-11-05
发明人
CAULFIELD MICHAEL;CUPO ALBERT;DEAN HANSI;HOFFENBERG SIMON;KING C RICHTER;KLASSE P J;MAROZSAN ANDRE;MOORE JOHN P;SANDERS ROGIER W;WARD ANDREW;WILSON IAN;JULIEN JEAN-PHILIPPE;
申请人
CORNELL UNIVERSITY;INTERNATIONAL AIDS VACCINE INITIATIVE;SCRIPPS RESEARCH INSTITUTE;
主分类号
IPC分类号
C07K-014/005
摘要
(US9738688) The present application relates to novel HIV-1 envelope glycoproteins, which may be utilized as HIV-1 vaccine immunogens, and antigens for crystallization, electron micrsocopy and other biophysical, biochemical and immunological studies for the identification of broad neutralizing antibodies.  The present invention encompasses the preparation and purification of immunogenic compositions, which are formulated into the vaccines of the present invention.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2012US-61722739 2012-11-05 2013US-14072209 2013-11-05
主权利要求
(US9738688) What is claimed is: 1.  An engineered or non-naturally occurring HIV-1 envelope glycoprotein isolated from a BG505 virus and having a SOSIP mutation wherein the glycoprotein is a BG505 SOSIP.664 gp140 trimer   and   wherein the SOSIP mutation comprises one or more mutations of the wild type amino acid alanine (A) or by wild type amino acid threonine (T) substitution with cysteine (C) (SOS mutation) and/or a mutation of the wild type amino acid isoleucine (I) by substitution with proline (P) (IP mutation);   wherein the BG505 SOSIP.664 gp140 trimer is more compact than a KNH1144 SOSIP.664G trimer isolated from with respect to envelope volume; and   wherein the BG505 SOSIP.664 gp140 trimer has a thermal denaturation midpoint (TM) of about 68 C. 2. An engineered or non-naturally occurring HIV-1 envelope glycoprotein isolated from a BG505 virus and having a SOSIP mutation, wherein the SOSIP mutation comprises a: (a) mutation of the wild type amino acid threonine (T) by substitution with asparagine (N) to create 2G12, and PGT125, PGT126, PGT127, PGT128, PGT129, PGT130, and/or PGT 131 epitopes;   (b) mutation of the wild type amino acid isoleucine (I) by substitution with proline (P) to facilitation trimerization; or   (c) mutations of the wild type sequence REKR (SEQ ID NO: 1) at amino acid positions by substitution with RRRRRR (SEQ ID NO: 2) to enhance cleavage;   or any combination thereof. 3. An engineered or non-naturally occurring HIV-1 envelope comprising the amino acid sequence of SEQ ID NO: 3. 4. The glycoprotein of claim 1, wherein the SOSIP mutation comprises a mutation of the wild type amino acid alanine (A) at position 498 of SEQ ID NO: 5 by substitution with cysteine (C) (SOS mutation). 5. The glycoprotein of claim 1, wherein the SOSIP mutation comprises a mutation of the wild type amino acid threonine (T) at position 602 of SEQ ID NO: 5 by substitution with cysteine (C) (SOS mutation). 6. The glycoprotein of claim 1, wherein the SOSIP mutation comprises a mutation of the wild type amino acid isoleucine (I) at position 556 of SEQ ID NO: 5 by substitution with proline (P) (IP mutation). 7. The glycoprotein of claim 1, wherein the SOSIP mutation comprises a mutation of the wild type amino acid alanine (A) at position 498 of SEQ ID NO: 5 by substitution with cysteine (C) and of the wild type amino acid threonine (T) at position 602 of SEQ ID NO: 5 by substitution with cysteine (C) (SOS mutation) and a mutation of the wild type amino acid isoleucine (I) at position 556 of SEQ ID NO: 5 by substitution with proline (P) (IP mutation). 8. The glycoprotein of claim 2, wherein the SOSIP mutation comprises a mutation of the wild type amino acid threonine (T) at position 330 of SEQ ID NO: 5 by substitution with asparagine (N). 9. The glycoprotein of claim 2, wherein the SOSIP mutation comprises a mutation of the wild type amino acid isoleucine (I) at position 556 of SEQ ID NO: 5 by substitution with proline (P). 10. The glycoprotein of claim 2, wherein the SOSIP mutation comprises mutations of the wild type sequence REKR (SEQ ID NO: 1) at amino acid positions 505-508 of SEQ ID NO: 5 by substitution with RRRRRR (SEQ ID NO: 2). 11. The glycoprotein of claim 2, wherein the SOSIP mutation comprises a (a) mutation of the wild type amino acid threonine (T) at position 330 of SEQ ID NO: 5 by substitution with asparagine (N);   (b) mutation of the wild type amino acid isoleucine (I) at position 556 of SEQ ID NO: 5 by substitution with proline (P); and   (c) mutations of the wild type sequence REKR (SEQ ID NO: 1) at amino acid positions 505-508 of SEQ ID NO: 5 by substitution with RRRRRR (SEQ ID NO: 2). 12. The glycoprotein of claim 3 consisting essentially of the amino acid sequence of SEQ ID NO: 3. 13. The glycoprotein of claim 3 consisting of the amino acid sequence of SEQ ID NO: 3. 14. A method of eliciting an immune response comprising administering to a mammal the glycoprotein of claim 1. 15. The method of claim 14 further comprising an adjuvant. 16. The method of claim 15 wherein the adjuvant comprises alum. 17. A method of eliciting an immune response comprising administering to a mammal the glycoprotein of claim 3. 18. The method of claim 17 further comprising an adjuvant. 19. The method of claim 18 wherein the adjuvant comprises alu
法律状态
(US9738688) LEGAL DETAILS FOR US2014212458  Actual or expected expiration date=2033-11-05    Legal state=ALIVE    Status=GRANTED     Event publication date=2013-11-05  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US14072209  Application date=2013-11-05  Standardized application number=2013US-14072209     Event publication date=2013-11-05  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: SNYDER, STUART    Event publication date=2013-11-05  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1648     Event publication date=2013-11-05  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: 43094.01.2026 Customer Nbr: 99562    Event publication date=2013-11-05  Event code=US/ENT  Event type=Administrative notifications  Business Entity Status: SMALL    Event publication date=2013-11-05  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=2013-11-05  Event code=US/ENTYSTA  Event type=Administrative notifications  Entity Status Set to Undiscounted    Event publication date=2013-11-19  Event code=US/SMALL  Event type=Administrative notifications  Appl Has Filed a Verified Statement of Micro to Small Entity Status    Event publication date=2013-11-25  Event code=US/APPINC  Event type=Examination events  Event type=OAO  Notice Mailed--Application Incomplete--Filing Date Assigned    Event publication date=2013-11-25  Event code=US/APPFILEREC  Event type=Administrative notifications  Event type=OAO  Filing Receipt    Event publication date=2014-03-12  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF  Effective date of the event=2014-03-10  CONFIRMATORY LICENSE ASSIGNOR:CORNELL UNIVERSITY / CORNELL RESEARCH FOUNDATION, INC. REEL/FRAME:032436/0727     Event publication date=2014-03-25  Event code=US/PTARDY  Event indicator=Pos  Event type=Examination events  Patent Term Adjustment - Ready for Examination    Event publication date=2014-04-22  Event code=US/RECEIPTUP  Event type=Administrative notifications  Event type=OAO  Filing Receipt - Updated    Event publication date=2014-05-27  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner in GAU    Event publication date=2014-07-31  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2014212458  Publication stage Code=A1  Publication date=2014-07-31  Standardized publication number=US20140212458     Event publication date=2014-07-31  Event code=US/PGPUBN  Event indicator=Pos  Event type=Examination events  Event type=OAO  PG-Pub Issue Notification    Event publication date=2014-11-07  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2015-01-30  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: THE SCRIPPS RESEARCH INSTITUTE, CALIFORNIA ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:WARD, ANDREW WILSON, IAN JULIEN, JEAN-PHILIPPE SIGNING DATES FROM 20140328 TO 20140401 REEL/FRAME:034852/0291     Event publication date=2015-01-30  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: CORNELL UNIVERSITY, NEW YORK ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:CUPO, ALBERT KLASSE, P. J. MAROZSAN, ANDRE AND OTHERS SIGNING DATES FROM 20140407 TO 20140515 REEL/FRAME:034852/0421     Event publication date=2015-01-30  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2015-02-02  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: INTERNATIONAL AIDS VACCINE INITIATIVE, NEW YORK ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:CAULFIELD, MICHAEL DEAN, HANSI HOFFENBERG, SIMON AND OTHERS SIGNING DATES FROM 20140411 TO 20140418 REEL/FRAME:034863/0767     Event publication date=2015-02-09  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2015-02-17  Event code=US/RECEIPTCOR  Event type=Administrative notifications  Event type=OAO  Filing Receipt - Corrected    Event publication date=2015-06-30  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner in GAU    Event publication date=2015-09-02  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner in GAU    Event publication date=2015-09-30  Event code=US/CTFR  Event type=Examination events  Event type=OA  Event type=OAO  Final Rejection    Event publication date=2016-04-07  Event code=US/RCEX  Event type=Examination events  Event type=RCE  Event type=OAI  Request for Continued Examination (RCE)    Event publication date=2016-04-14  Event code=US/DRCECAP  Event type=RCE  Event type=OAO  Disposal for a RCE / CPA / R129    Event publication date=2016-08-22  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2017-03-31  Event code=US/EXAC  Event type=Examination events  Event type=OAO  Examiner's Amendment Communication    Event publication date=2017-04-10  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=2017-04-11  Event code=US/ANA  Event type=Corrections  Event type=Examination events  Event type=OA  Amendment after Notice of Allowance (Rule 312)    Event publication date=2017-04-25  Event code=US/N271  Event type=Examination events  Event type=OAO  Response to Amendment under Rule 312    Event publication date=2017-04-27  Event code=US/ANA  Event type=Corrections  Event type=Examination events  Event type=OA  Amendment after Notice of Allowance (Rule 312)    Event publication date=2017-05-12  Event code=US/N271  Event type=Examination events  Event type=OAO  Response to Amendment under Rule 312    Event publication date=2017-06-30  Event code=US/APRDY  Event indicator=Pos  Event type=Examination events  Application Is Considered Ready for Issue    Event publication date=2017-08-02  Event code=US/APST  Event indicator=Pos  Event type=Examination events  PAIR Application Status    Event publication date=2017-08-02  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Issue Notification Mailed    Event publication date=2017-08-22  Event code=US/B2  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as second publication  Publication country=US  Publication number=US09738688  Publication stage Code=B2  Publication date=2017-08-22  Standardized publication number=US9738688
专利类型码
A1B2
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