Immunologic detecting method 机翻标题: 暂无翻译,请尝试点击翻译按钮。

源语言标题
(JP05049494) 免疫​学​的​検出​方法
公开号/公开日
JPH0549494 A 1993-03-02 [JP05049494] / 1993-03-02
申请号/申请日
1991JP-0183548 / 1991-06-28
发明人
JIINNMARUKU SHIYURAETSUPI;KURAUSU RAMUSHIYUTAINERU;BUIRII MEIERU;
申请人
CIBA GEIGY;
主分类号
IPC分类号
A01N-047/36C07D-251/16C07K-001/113C07K-014/76C07K-016/00C07K-016/44C12P-021/08C12R-001/91G01N-033/53G01N-033/531G01N-033/577
摘要
(JP05049494) The present invention relates to monoclonal antibodies which are distinguished by high selectivity and affinity for triasulfuron and which are therefore outstandingly suitable for use in an immunoassay for the rapid and efficient detection of triasulfuron.  Another aspect of the present invention relates to hybridoma cell lines which produce said monoclonal antibodies and to immunological methods for detecting triasulfuron in samples of soil, water or air using said monoclonal antibodies and to the assay kits which can be used within the scope of these detection methods.  (From EP0463998 A2)
机翻摘要
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地址
代理人
代理机构
;
优先权号
1990CH-0002173 1990-06-29 1990CH-0004078 1990-12-21
主权利要求
(JP05049494)  1. (A) Sulfonamide part of target molecule truth The connectivity component which is included qualitatively compounds with the suitable high-molecular quantitative carrier molecule It is done, (B) The provision animal following, (a) with the mixture which is produced exemption The *** impression work it is done, (C) The immunity qualified B cell the immunity impression work from the provision animal which is done single Separating, (D) The tumor which the said immunity qualified B cell can separate the cell continually The cell it is fused, (E) Fusion ones which it forms to be isolated, and after the selection, the place Produces the gazing/hoping antibody the hybridoma cell which cloning [re], and (F) In order to produce the monoclonal antibody said [haiburi] The [doma] cell is cultured with the test inside of pipe or in vivo, In 1 kinds or the sulfonyl urea herbicide above that which consist of Confronting, [monokurona] which possesses high-level peculiarity and affinity Production method of [ru] antibody.  2. The aforementioned sulfonamide part substitution aryl Method of the claim 1 statement which is the sulfonyl basis.  3. The aforementioned aryl basis phenyl group and [pirijiru] The claim 2 statement which is the basis, the [chieniru] basis or the [pirazoriru] basis Method.  4. The aforementioned sulfonyl urea herbicide the next formula (A): [Conversion 1] When (in formula, n is integer of 1 or 10, as for R CO OH is displayed, and the place where n is integer of 2 or 10 Combination and R COOH and NH [2] or display SH) with displaying In 4 rank of [ru] triazine ring R- (CH [2]) [n] - [O]- possession The claim either 1 which is the chemical compound which it does or 3 in 1 sections description Method of *** .  5. As a sulfonamide part the next formula (B): [Conversion 2] (In formula, R' COOH and NH [2] or to display SH, the [so] Doing, n displays the integer of 1 or 10) with the stabilization which is displayed The claim either 1 where the fragment which is done is used or 4 1 In section method of statement.  6. Formula (A) R in NH [to display 2], and The claim 1 where n displays the integer of 2 or 6 or 5 in each case Or in 1 sections method of statement.  7. R NH [displays 2], and n displays 3 Method of claim 6 statement.  8. Formula (B) R in' to display COOH, the [so] Doing, the claim 1 where n displays the integer of 1 or 6 or 7 it is, Gap in 1 sections method of statement.  9. R' to display COOH, and n 2 Method of the claim 8 statement which you display.  10. Claim either 1 or 9 in 1 sections description Following to the method of the *** , it was produced, 1 kinds or [su] above that Vis-a-vis the [ruhoniru] urea herbicide high-level peculiarity and affinity The monoclonal antibody which it possesses.  11. Vis-a-vis thoria sail freon the high-level uniqueness To possess characteristic and affinity, and very many structural Seki The thoria sail freon analog and the intersection reactivity which ream are done substantial The hybridoma which produces the monoclonal antibody which does not show Cell system.  12. Vis-a-vis thoria sail freon the high-level uniqueness To possess characteristic and affinity, and [purimisuruhuron], the sail [hometsuron] - methyl and [toribenuron] - methyl and [chiametsu] Remaining overnight - methyl and [kurorosuruhuron], Venn sail freon - [me] Chill and [metsusuruhuron] - methyl and [nikosuruhuron] - [mechi] The structure which is selected from the group which consists of [ru] and DPX9636 [mo] which does not show chemical compound and the intersection reactivity which it is related to the mark substantially [haiburi] of the claim 11 statement which produces the [nokuronaru] antibody [doma] cell system.  13. Vis-a-vis thoria sail freon the high-level uniqueness To possess characteristic and affinity, and [purimisuruhuron], the sail [hometsuron] - methyl and [toribenuron] - methyl and [chiametsu] Remaining overnight - methyl and [kurorosuruhuron], Venn sail freon - [me] Chill and [metsusuruhuron] - methyl and [nikosuruhuron] - [mechi] The structure which is selected from the group which consists of [ru] and DPX9636 The thoria sail freon analog which it is related to the mark under 1.0% The claim which produces the monoclonal antibody which shows intersection reactivity Hybridoma cell system of section 11 statement.  14. The feature of ECACC 90,021,704 Hybridoma cell system of the claim 13 statement which it possesses and Those clones and sub clone.  15. The feature of ECACC9002 1703 Hybridoma cell system of the claim 13 statement which it possesses and Those clones and sub clone.  16. Vis-a-vis thoria sail freon the high-level uniqueness To possess characteristic and affinity, and [purimisuruhuron], the sail [hometsuron] - methyl and [toribenuron] - methyl and [chiametsu] Remaining overnight - methyl and [kurorosuruhuron], Venn sail freon - [me] Chill and [metsusuruhuron] - methyl and [nikosuruhuron] - [mechi] The structure which is selected from the group which consists of [ru] and DPX9636 The thoria sail freon analog which it is related to the mark under 0.1% The claim which produces the monoclonal antibody which shows intersection reactivity Hybridoma cell system of section 11 statement.  17. The feature of ECACC 90,021,704 Hybridoma cell system of the claim 16 statement which it possesses and Those clones and sub clone.  18. It possesses the feature of starting ones still, namely Vis-a-vis 1 kinds or the sulfonyl urea herbicide above that high The monoclonal antibody which possesses the peculiarity and affinity of degree The claim whose synthesis and the secretion to in the nutrient medium around still are possible Section either 11 or 17 in 1 sections [haiburido] of statement Mutation body or variety of [ma] cell type.  19. Vis-a-vis thoria sail freon the high-level uniqueness To possess characteristic and affinity, and very many structural Seki The thoria sail freon analog and the intersection reactivity which ream are done substantial The monoclonal antibody or that derivative which does not show.  20. Vis-a-vis thoria sail freon the high-level uniqueness To possess characteristic and affinity, and [purimisuruhuron], the sail [hometsuron] - methyl and [toribenuron] - methyl and [chiametsu] Remaining overnight - methyl and [kurorosuruhuron], Venn sail freon - [me] Chill and [metsusuruhuron] - methyl and [nikosuruhuron] - [mechi] [tori] which is selected from the group which consists of [ru] and DPX9636 The [asuruhuron] analog and the receiving which does not show intersection reactivity substantially Monoclonal antibody or that derivative of seeking section 19 statement.  21. Vis-a-vis thoria sail freon the high-level uniqueness To possess characteristic and affinity, and [purimisuruhuron], the sail [hometsuron] - methyl and [toribenuron] - methyl and [chiametsu] Remaining overnight - methyl and [kurorosuruhuron], Venn sail freon - [me] Chill and [metsusuruhuron] - methyl and [nikosuruhuron] - [mechi] The structure which is selected from the group which consists of [ru] and DPX9636 The thoria sail freon analog which it is related to the mark under 1.0% Monoclonal resistance of the claim 19 statement which shows intersection reactivity Body or that derivative.  22. The feature of ECACC 90,021,704 The claim 21 which is obtained from the hybridoma cell system which it possesses Monoclonal antibody or that derivative of statement.  23. The feature of ECACC 90,021,704 The claim 21 which is obtained from the [haiburidoru] cell system which it possesses Monoclonal antibody or that derivative of statement.  24. Vis-a-vis thoria sail freon the high-level uniqueness To possess characteristic and affinity, and [purimisuruhuron], the sail [hometsuron] - methyl and [toribenuron] - methyl and [chiametsu] Remaining overnight - methyl and [kurorosuruhuron], Venn sail freon - [me] Chill and [metsusuruhuron] - methyl and [nikosuruhuron] - [mechi] The structure which is selected from the group which consists of [ru] and DPX9636 The thoria sail freon analog which it is related to the mark under 0.1% Monoclonal resistance of the claim 19 statement which shows intersection reactivity Body or that derivative.  25. The feature of ECACC 90,021,704 The claim 24 which is obtained from the hybridoma cell system which it possesses Monoclonal antibody or that derivative of statement.  26. (A) -suitableness thoria sail freon connectivity forming Amount is synthesized, and the carrier molecule is compounded, (B) The provision animal the immunity impression work is done with the said mixture, (C) The immunity qualified B cell the immunity impression work from the provision animal which is done single Separating, (D) The tumor which the said immunity qualified B cell can separate the cell continually Cell system it is fused, (E) Fusion ones which it forms to be isolated, in the suitable culture nutrient medium To be cultured, and the positive hybridoma cell cloning To be done, and (F) The hybridoma cell which produces the antibody of desire selects It is done, [haiburidoma] cell system of claim 11 statement make Structure method.  27. Connection with the sulfonyl urea connectivity component counter To include the reaction basis which is utilized freely in application/response, and said [suruhoni] Can grant immunogenicity to the [ru] urea component the giant molecule chemical compound which bearing The claim 1 which is used as the body molecule or 9 or 26 In each case in 1 sections method of statement.  28. Molecular weight 10000 or 15000 The claim 27 where the protein which is rich in the ricin of 00 is used Method of statement.  29. Carrier molecule of sulfonyl urea connectivity component To compound happens directly or, or the reaction basis and phase of the carrier molecule The *** [supe] which possesses 1 kinds or the reaction basis above that which can operate [sa] fragment (the spacer) through, the claim 1 which happens it is not It does, either 9 or 26 in 1 sections method of statement.  30. The aforementioned reaction basis carboxyl group, amino Method of the claim 29 statement which is the basis or the SH basis.  31. The aforementioned sulfonyl urea connectivity component the next formula (A): [Conversion 3] When (in formula, n is integer of 1 or 10, as for R CO OH is displayed, and the place where n is integer of 2 or 10 Combination and R COOH and NH [2] or display SH) with displaying In 4 rank of [ru] triazine ring R- (CH [2]) [n] - [O]- possession Method of the claim 26 statement which is the chemical compound which it does.  32. The aforementioned sulfonyl urea connectivity component the next formula (B): [Conversion 4] (In formula, R' COOH and NH [2] or to display SH, the [so] Doing, n displays the integer of 1 or 10) with the chemical compound which is displayed So method of the claim 26 statement which is.  33. Connection reaction active ester method or [jia] The claim 1 which is done making use of [zoniumu] method or 9 or 2 Either of 6 in 1 sections method of statement.  34. Immunity impression work of provision animal connectivity component and bearing The body molecule and the mixture which consists of 1 times or in dosage above that Rather than the claim either 1 which is done or 9 or 26 1 In section method of statement.  35. Dosage the injection and the peritoneum intracavitary injection inside the vein As for the [ku] the receiving which is done with form of the subcutaneous injection or those combinations Method of seeking section 34 statement.  36. HAT selection media fusion hybrid detail The claim 1 which is used for selecting the placenta or 9 or 2 Either of 6 in 1 sections method of statement.  37. Positive monoclonal antibody production characteristic [haibu] The lid cell culture to be separated by limited dilution method, next suitable In this name culture nutrient medium to clone the claim 1 which is converted or 9 [ma] It is either of 26 in 1 sections method of statement.  38. Following to the method of claim 37 statement, the claw To [n] [monoku] where the hybridoma cell clone which is converted is suitable With the immunoassay for the formation of the low null antibody test The claim either 1 which is done or 9 or 26 in 1 sections description Method of *** .  39. Claim either 11 or 18 1 sections [haiburidoru] cell system of statement in vivo or test inside of pipe The monochrome which is cultured so by known method, and is produced The monoclonal antibody where the [naru] antibody is isolated manufacturing Law.  40. Monoclonal antibody of claim 10 statement The sulfonyl urea herbicide which is used immunology detection Law.  41. Claim either 19 or 25 1 sections [toriasuruhu] where the monoclonal antibody of statement is used Immunology method of detection of remaining overnight.  42. Is used the monoclonal antibody which, E [haiburido] which possesses the feature of CACC90021702 From [ma] cell system or that clone or sub clone Immunology of the thoria sail freon of the claim 41 statement which is obtained Method of detection.  43. Is used the monoclonal antibody which, E [haiburido] which possesses the feature of CACC90021703 From [ma] cell system or that clone or sub clone Immunology of the thoria sail freon of the claim 41 statement which is obtained Method of detection.  44. Is used the monoclonal antibody which, E [haiburido] which possesses the feature of CACC90021704 From [ma] cell system or that clone or sub clone Immunology of the thoria sail freon of the claim 41 statement which is obtained Method of detection.  45. The claim 4 which is competitive immunoassay Either 0 or 44 in 1 sections method of statement.  46. Usual supporting material, reagent and other attachments Other than the adding medicine, as a reagent [monokurona] of claim 10 statement The [ru] antibody is contained 1 kinds at least, for using adjustment The [re] te the sulfonyl urea herbicide which is form of the test kit which is Composition for immunology detection.  47. Usual supporting material, reagent and other attachments Other than the adding medicine, claim 19 or 25 in each case as a reagent Or the monoclonal antibody of statement at least in 1 sections 1 kind containing Form of the test kit which it possesses, is adjusted for using The composition for immunology detection of thoria sail freon which is.  48. Usual supporting material, reagent and other attachments Other than adding medicine, the feature of ECACC 90,021,704 possession Hybridoma cell system or that clone which it does or The monoclonal antibody which is obtained from the sub clone little Also 1 kinds it contains, you adjust for using the claim 47 statement The thoria sail freon which is form of the test kit which is done Composition for immunology detection.  49. Usual supporting material, reagent and other attachments Other than adding medicine, the feature of ECACC 90,021,704 possession Hybridoma cell system or those clones which it does As for the [ku] the monoclonal antibody which is obtained from the sub clone little Also without 1 kinds it contains, for using the claim 47 statement The thoria sail flow which is form of the test kit which is adjusted Composition for immunology detection of [n].  50. Usual supporting material, reagent and other attachments Other than adding medicine, the feature of ECACC 90,021,704 possession Hybridoma cell system or those clones which it does As for the [ku] the monoclonal antibody which is obtained from the sub clone little Also without 1 kinds it contains, for using the claim 47 statement [toriasuruhu] which is form of the test kit which is adjusted Composition for immunology detection of remaining overnight.  51. The next formula (A): [Conversion 5] When (in formula, n is integer of 1 or 10, as for R CO OH is displayed, and the place where n is integer of 2 or 10 Combination and R COOH and NH [2] or display SH) with displaying In 4 rank of [ru] triazine ring R- (CH [2]) [n] - [O]- possession The thoria sail freon connectivity component which it does.  52. R NH [displays 2,] and 2 there is no n Thoria sail freon ream of the claim 51 statement which it does and displays the integer of 6 Joining characteristic component.  53. R NH [to display 2], and n 3 the chart The thoria sail freon connectivity component of the claim 52 statement which it does.  54. The next formula (B): [Conversion 6] (In formula, R' COOH and NH [2] or to display SH, the [so] Doing, n displays the integer of 1 or 10) with the thoria which is displayed Sail freon connectivity component.  55. R' to display COOH, and n 1 Or thoria sail flow of the claim 54 statement which displays the integer of 6 [n] connectivity component.  56. R' to display COOH, and n 2 The thoria sail freon connectivity component of the claim 55 statement which is displayed.  57. Sulfonyl urea herbicide itself known Hydrolysis ardently in the sulfonamide functional part with method It can a debeaker, and that way - SO [2] NH [2] the basis To react with the reactivity carboxylic acid derivative, and when The [ri], the COOH end group which is formed with known method NH [2] the basis Or it consists of the fact that it converts to the SH basis, sulfonyl urea To include the sulfonamide part of the molecule substantially, at the same time suitableness Being connected by the high-molecular quantitative carrier molecule, in the provision animal the description above sulfonic Inducing the unique immuneresponse which is directed vis-a-vis the [namido] part Production method of the sulfonyl urea connectivity component which it can do.  58. The aforementioned connectivity component the next formula (B):  [Conversion 7]  (In formula, R' COOH and NH [2] or to display SH, the [so] Doing, n displays the integer of 1 or 10) with the thoria which is displayed Method of the claim 38 statement which is the sail freon connectivity component.
法律状态
(JP05049494) LEGAL DETAILS FOR JPH0549494  Actual or expected expiration date=2002-03-02    Legal state=DEAD    Status=LAPSED     Event publication date=1991-06-28  Event code=JP/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=JP JP18354891  Application date=1991-06-28  Standardized application number=1991JP-0183548     Event publication date=1993-03-02  Event code=JP/A  Event indicator=Pos  Event type=Examination events  Published application  Publication country=JP  Publication number=JPH0549494  Publication stage Code=A  Publication date=1993-03-02  Standardized publication number=JP05049494     Event publication date=2002-03-02  Event code=JP/EEDL  Event indicator=Neg  Event type=Event indicating Not In Force  Pending application likely abandoned
专利类型码
A
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