(CN109053520) Extraction, separation and purification method of astaxanthin in rhodotorula mucilaginosa 机翻标题: 暂无翻译,请尝试点击翻译按钮。

源语言标题
(CN109053520) Extraction, separation and purification method of astaxanthin in rhodotorula mucilaginosa
公开号/公开日
CN109053520CN109053520 / 2020-09-012018-12-21
申请号/申请日
CN201811096521 / 2018-09-19
发明人
XU CHUNHOULIU YINGXIE WEITIANKANG KAIGE YE;
申请人
GUANGDONG OCEAN UNIVERSITY;
主分类号
IPC分类号
C07C-403/24
摘要
(CN109053520) The invention discloses an extraction, separation and purification method of astaxanthin in rhodotorula mucilaginosa. The method comprises the following steps: S1, performing wall breaking on dried thallus cells of rhodotorula mucilaginosa by an acid heating method, performing centrifuging, discarding supernatant, performing washing, adding a leaching solution, and performing extraction at 45-55 DEG C for 2-4 times, so as to obtain an astaxanthin coarse extraction solution; S2, adding a KOH alcohol solution to the astaxanthin coarse extraction solution, and performing saponification under thedark condition of 4-50 DEG C for 20 minutes to 7 hours, so as to obtain an astaxanthin extraction solution after saponification; S3, feeding samples of the astaxanthin extraction solution into a silica gel column chromatography, performing gradient elution with a mobile phase, and performing separation so as to obtain astaxanthin finished products. According to the method disclosed by the invention, a wall breaking method, a leaching solvent, a saponification method and the extraction condition are improved, the obtained astaxanthin is high in yield and purity; in addition, the separation speed is increased, reduction of destruction of astaxanthin in the course of wall breaking and saponification is facilitated, the physiological activity is good, and industrial production can be realized.
机翻摘要
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地址
代理人
(CN109053520B) LIU YAOYUN ; YOGO PATENT & TRADEMARK AGENCY LIMITED COMPANY Reg. Nb: 44102 CHEN WEIBIN ; YOGO PATENT & TRADEMARK AGENCY LIMITED COMPANY Reg. Nb: 44102
代理机构
;
优先权号
2018CN-1096521
主权利要求
(CN109053520) 1.一种胶红酵母中虾青素提取及分离纯化的方法,其特征在于,包括以下步骤:   S1.采用酸热法将胶红酵母干菌体细胞破壁后,离心,弃上清液,洗涤,加入浸提液,于45~55℃提取2~4次,得到虾青素粗提液;其中所述浸提液为乙酸乙酯与乙醇的混合液,所述乙酸乙酯与乙醇的体积比为0.5~3.5:1;   所述酸热法的条件为:向胶红酵母干菌体细胞中加入2~4mol/L的盐酸,混匀,室温静置30~50min,沸水浴1~5min进行破壁;   S2.向虾青素粗提液中加入KOH乙醇溶液,在4~50℃黑暗条件下皂化20min~7h后,得到皂化后的虾青素提取液;所述KOH乙醇溶液的浓度为10~20g/L;所述虾青素粗提液与KOH乙醇溶液的体积比为1:0.6~2;   S3.将虾青素提取液上样至硅胶柱层析中,以流动相进行梯度洗脱,分离得到虾青素成品;其中,所述流动相为正己烷、石油醚与乙酸乙酯的混合液,所述正己烷、石油醚与乙酸乙酯的体积比为5~7:1~2:1~2;或者所述流动相为正己烷与丙酮的混合液,所述正己烷与丙酮的体积比为2~7:1~3。 2.根据权利要求1所述的方法,其特征在于,所述胶红酵母干菌体细胞与盐酸的料液比为0.1:3~7g/mL。 3.根据权利要求1所述的方法,其特征在于,步骤S1中,所述胶红酵母干菌体细胞与浸提液的料液比为0.1:5~10g/mL。 4.根据权利要求1所述的方法,其特征在于,步骤S2中,所述皂化的条件为在20~40℃黑暗条件下皂化30~60min。 5.根据权利要求1~4任一所述的方法,其特征在于,还包括收集所述胶红酵母干菌体细胞的步骤:将胶红酵母发酵液离心后,弃上清液,用灭菌蒸馏水洗涤2~4次,将沉淀细胞于40~60℃烘干至恒重,即可得到所述胶红酵母干菌体细胞。
法律状态
GRANTED
专利类型码
BA
国别省市代码
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