防止​基因​漂移​的​水稻​工程​保持​系​的​创制​方法​及其​应用

源语言标题
(CN105063083) 防止​基因​漂移​的​水稻​工程​保持​系​的​创制​方法​及其​应用
公开号/公开日
CN105063083 A 2015-11-18 [CN105063083] / 2015-11-18
申请号/申请日
2015CN-0417227 / 2015-07-16
发明人
SONG SHUFENG; LI XINQI; LI LI; ZHANG DABING; FU XIQIN; YUAN DINGYANG; YUAN LONGPING
申请人
HUNAN HYBRID RICE RESEARCH CENTER
主分类号
IPC分类号
A01H-001/02;A01H-005/00;C12N-015/29;C12N-015/65;C12N-015/82
摘要
(CN105063083)
本​发明​公开​了​防止​基因​漂移​的​水稻​工程​保持​系​的​创制​方法,包括:步骤​一、将​外源​报告​基因​和​花粉​致死​基因​定点​插入​到​野生​型​水稻​染色体​上​育​性​调控​基因​的​侧翼​序列,得到​含有​外源​报告​基因​和​花粉​致死​基因​的​转基因​株系,其中,外源​报告​基因、花粉​致死​基因​与​育​性​调控​基因​能够​紧密​连锁​遗传;以及,步骤​二、将​野生​型​水稻​与​纯​合​隐性​水稻​普通​雄性​核​不育​株系​杂交​得到​F1​代​植株,之后​将​F1​代​植株​作为​父本​与​转基因​株系​杂交​得到​F2​代,从​F2​代​中​筛选​含有​外源​报告​基因​的​杂​合​株系​即为​所需​水稻​工程​保持​系,其中,纯​合​隐性​水稻​普通​雄性​核​不育​株系​为​育​性​调控​基因​的​突变​体​株系。本​发明​还​公开​了​防止​基因​漂移​的​水稻​工程​保持​系在​水稻​育种​中的​应用。
地址
代理人
代理机构
优先权号
2015CN-0417227 2015-07-16
主权利要求
(CN105063083)
1. A rice plant engineering maintainer for preventing drift producing method of a gene, characterized in, including: Step one, the foreign reporter gene and a pollen lethal gene on the chromosome ZmAA1 point inserted into the wild-type rice flanking sequences of a gene regulated Fertilitv, lethal gene containing a foreign reporter gene and a pollen obtained ZmAA1 transgenic lines, wherein, a foreign reporter gene, pollen can be closely linked with the lethal gene regulatory gene ZmAA1 Fertilitv genetic; and, Step two, taken as the female parent transgenic lines obtained in step one, one of ordinary rice paddy rice and wild-type mice homozygous recessive male sterile lines F1 progeny plants obtained after hybridization nucleuses, F1 progeny plants after used as a female transgenic lines obtained after hybridization with the male F2 generation, from the F2 generation contains an exogenous reporter gene in the heterozygous for screening genetic locus fertility xBrassicoraphanus rice engineering maintainer line that is required, wherein, the male sterile lines into ordinary rice homozygous recessive mutant strains Fertilitv regulatory gene.
法律状态
(CN105063083)
LEGAL DETAILS FOR CN105063083

Actual or expected expiration date=2035-07-16   
Legal state=ALIVE   
Status=PENDING 



Event publication date=2015-07-16 
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Event type=Examination events 
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Application country=CN CN201510417227 
Application date=2015-07-16 
Standardized application number=2015CN-0417227 



Event publication date=2015-11-18 
Event code=CN/A 
Event type=Examination events 
Published application

Publication country=CN 
Publication number=CN105063083 
Publication stage Code=A 
Publication date=2015-11-18 
Standardized publication number=CN105063083 



Event publication date=2015-11-18 
Event code=CN/C06 
Event indicator=Pos 
Event type=Examination events 
Publication



Event publication date=2015-12-16 
Event code=CN/C10 
Event type=Examination events 
Request of examination as to substance
专利类型码
A
国别省市代码
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