The Effect of Explant Types and Plant Growth Regulators On Callus Induction of Geranium (Pelargonium graveolens L'Her) In Vitro 机翻标题: 暂无翻译,请尝试点击翻译按钮。

会议集名/来源
IOP Conference Series: Earth and Environmental Science
出版年
Dec 20, 2019
语种
English
会议日期
2019-08-05 - 2019-08-07
作者
Huda, Moch Faizul 1 ; Indriyani, S. 2 ; Widoretno, W. 2 1 Student of Biology Department, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Indonesia 2 Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Indonesia wahyu_widoretno@yahoo.com
摘要
The purpose of this study was to determine the effect of explant types and plant growth regulators on callus induction of geranium (Pelargonium graveolens L'Her). Callus induction was carried out by culturing leaf and petiole explants from ex vitro geraniums on MS + 0.5 mg/l 2.4-D + 0.1 and 0.3 mg/l Benzyladenine (BA) or Kinetin. Each culture was carried out in 5 replications, cultures were incubated in rooms 25°C and 600 lux. The parameters days for callus induction and percentage of callus formation were observed. Geranium callus formation was influenced by types of explants and plant growth regulators. Petiole explants was able to induce callus better than leaf explants. Days for callus induction at second week and percentage of callus formation of petiole explants were 58 and 58% compared to leaf explants were only 53 and 54%. Plant Growth Regulators BA combined with 2.4-D was able to induce callus better than kinetin. The best callus formation was produced from petiole explants cultured on MS + 0.5 mg/l 2.4-D + 0.3 mg/l BA by 84%.
机翻摘要
暂无翻译结果,您可以尝试点击头部的翻译按钮。
关键词
Plants (botany) (major);Environmental engineering
若您需要申请原文,请登录。

最新评论

暂无评论。

登录后可以发表评论


意见反馈
返回顶部