Compositions and methods for preservation and storage of biological materials 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2017332622 A1 2017-11-23 [US20170332622] / 2017-11-23
申请号/申请日
2017US-15600445 / 2017-05-19
发明人
ELLIOTT GLORIA D;
申请人
UNIVERSITY OF NORTH CAROLINA;
主分类号
IPC分类号
A01N-001/02C12N-001/04
摘要
(US20170332622) In one aspect, solvent systems are described herein.  In some embodiments, such a solvent system comprises a disaccharide component, choline halide or choline acetate, and water.  The water is present in the solvent system in an amount of 5 weight percent to less than 25 weight percent, and the solvent system is free of crystals.  In another aspect, biomolecular compositions are described herein.  Such a composition can comprise one or more biological molecules dissolved in a solvent system described herein.  In yet another aspect, methods of storing a biological composition are described herein.  In some instances, such a method comprises contacting the biological composition with a liquid preservative composition, wherein the liquid preservative composition comprises a solvent system or biomolecular composition described herein.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2016US-62338770 2016-05-19 2017US-15600445 2017-05-19
主权利要求
(US20170332622) 1. A solvent system comprising: a disaccharide component, choline halide or choline acetate, and water, wherein the water is present in the solvent system in an amount of 5 weight percent to less than 25 weight percent, and the solvent system is free of crystals. 2. The solvent system of claim 1, wherein the disaccharide component comprises one or more species formed of two glucose units. 3. The solvent system of claim 2, wherein the disaccharide component comprises trehalose or trehalose derivative. 4. The solvent system of claim 1, wherein the solvent system comprises choline halide and the choline halide is choline chloride. 5. The solvent system of claim 1, wherein a molar ratio of the disaccharide component to choline halide or choline acetate ranges from 1:2 to 1:6. 6. The solvent system or claim 5, wherein the molar ratio is 1:4. 7. The solvent system of claim 1 having a glass transition temperature in a range of -40 deg. C. to -120 deg. C. 8. The solvent system of claim 1, wherein the water is present in an amount of 15 to 23 weight percent of the solvent system. 9. The solvent system of claim 1, wherein temperature dependence of viscosity of the solvent system displays Arrhenius behavior. 10. A biomolecular composition comprising: one or more biological molecules dissolved in a solvent system, the solvent system comprising a disaccharide component, choline halide or choline acetate, and water, wherein the water is present in the solvent system in an amount of 5 weight percent to less than 25 weight percent, and the solvent system is free of crystals. 11. The biomolecular composition of claim 10, wherein the one or more biological molecules is selected from the group consisting of proteins, enzymes and nucleic acids. 12. The biomolecular composition of claim 10, wherein the disaccharide component comprises one or more species formed of two glucose units. 13. The biomolecular composition of claim 12, wherein the disaccharide component comprises trehalose or trehalose derivative. 14. The biomolecular composition of claim 10, wherein the solvent system comprises choline halide and the choline halide is choline chloride. 15. The biomolecular composition of claim 10, wherein a molar ratio of the disaccharide component to choline halide or choline acetate ranges from 1:2 to 1:6. 16. The biomolecular composition of claim 15, wherein the molar ratio is 1:4. 17. The biomolecular composition of claim 10 having a glass transition temperature in a range of -40 deg. C. to -120 deg. C. 18. The biomolecular composition of claim 10, wherein the water is present in an amount of 15 to 23 weight percent of the solvent system. 19. A method of storing a biological composition comprising: contacting the biological composition with a liquid preservative composition, the liquid preservative composition including a disaccharide component, choline halide or choline acetate and water, wherein the water is present in the liquid preservative composition in an amount of 5 weight percent to less than 25 weight percent; and   converting the liquid preservative composition to an amorphous solid via cooling. 20. The method of claim 19, wherein the liquid preservative composition is cooled at a minimum critical cooling rate of 1.5 deg. C./min. 21. The method of claim 19, wherein the disaccharide component comprises one or more species formed of two glucose units. 22. The method of claim 21, wherein the disaccharide component comprises trehalose or trehalose derivative. 23. The method of claim 19, wherein the liquid preservative composition comprises choline halide and the choline halide is choline chloride. 24. The method of claim 19, wherein a molar ratio of disaccharide component to choline halide or choline acetate ranges from 1:2 to 1:6. 25. The method claim 19, wherein the liquid preservative composition is cooled and maintained at a temperature of -60 deg. C. to -90 deg. C. 26. The method of claim 19 further comprising heating the amorphous solid at a critical warming rate of 10 deg. C./min to 20 deg. C./min to return the preservative composition to liquid. 27. The method of claim 19, wherein the biological composition comprises biomolecules, viruses, bacteria, cells, engineered cells, tissues, micro-tissues, organs or microphysiological systems.
法律状态
(US20170332622) LEGAL DETAILS FOR US2017332622  Actual or expected expiration date=2037-05-19    Legal state=ALIVE    Status=PENDING     Event publication date=2017-05-19  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US15600445  Application date=2017-05-19  Standardized application number=2017US-15600445     Event publication date=2017-05-19  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: PYLA, PAUL D    Event publication date=2017-05-19  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1653     Event publication date=2017-05-19  Event code=US/SMALL  Event type=Administrative notifications  Appl Has Filed a Verified Statement of Micro to Small Entity Status Business Entity Status: SMALL    Event publication date=2017-05-19  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=Yes     Event publication date=2017-05-19  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: 5012918.019US1    Event publication date=2017-05-19  Event code=US/CUST  Event type=Examination events  Attorney/Agent Customer Number Customer Nbr: 11806    Event publication date=2017-05-19  Event code=US/ENT  Event type=Administrative notifications  Entity Status Set to Undiscounted    Event publication date=2017-05-26  Event code=US/SMALL  Event type=Administrative notifications  Appl Has Filed a Verified Statement of Micro to Small Entity Status    Event publication date=2017-06-01  Event code=US/APPFILEREC  Event type=Administrative notifications  Event type=OAO  Filing Receipt    Event publication date=2017-07-20  Event code=US/PTARDY  Event indicator=Pos  Event type=Examination events  Patent Term Adjustment - Ready for Examination    Event publication date=2017-07-31  Event code=US/RECEIPTUP  Event type=Administrative notifications  Event type=OAO  Filing Receipt - Updated    Event publication date=2017-11-23  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2017332622  Publication stage Code=A1  Publication date=2017-11-23  Standardized publication number=US20170332622     Event publication date=2017-11-24  Event code=US/PGPUBN  Event indicator=Pos  Event type=Examination events  Event type=OAO  PG-Pub Issue Notification    Event publication date=2018-03-12  Event code=US/DKNC  Event indicator=Pos  Event type=Examination events  Docketed New Case - Ready for Examination    Event publication date=2018-03-12  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=2018-04-23  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner
专利类型码
A1
国别省市代码
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