Compositions and methods for controlling insects 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
WO2004100971 A1 2004-11-25 [WO2004100971] / 2004-11-25
申请号/申请日
2004WO-US12947 / 2004-04-26
发明人
ENAN ESSAM;
申请人
VANDERBILT UNIVERSITY;
主分类号
IPC分类号
A01N-027/00A01N-031/04A01N-031/08A01N-031/16A01N-035/02A01N-035/06A01N-037/44A01N-043/30A01N-049/00A01N-065/00G01N-033/566
摘要
(WO2004100971) The present invention comprises compositions, methods and cell lines related to controlling insects.  An embodiment of a composition comprises a plant essential oil and targets at least one receptor of insects chosen from tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor, resulting in a change in the intracellular levels of cAMP, Ca2+, or both in the insects.
机翻摘要
暂无翻译结果,您可以尝试点击头部的翻译按钮。
地址
代理人
代理机构
;
优先权号
2003US-60465320 2003-04-24 2003US-60532503 2003-12-24
主权利要求
(WO2004100971) CLAIMS What is claimed is: 1. A composition for. controlling insects, comprising: at least two oils, wherein the composition targets at least one receptor of the insects chosen from tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor, resulting in a change in the intracellular levels of cAMP, Ca2+, or both in said insects. 2. The composition of claim 1, wherein the at least two oils comprise oils chosen from: t-anthole, black seed oil, camphene, carvacrol, d-carvone, 1-carvone, 1,8-cineole, p- cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil, lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di- hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p-quinone, α-thujone, thyme oil, and thymol. 3. The composition as recited in claim 2, wherein treatment with said composition has a repellent effect. 4. The composition as recited in claim 2, wherein treatment with said composition has a pesticidal effect. 5. The composition as recited in claim 2, and further comprising at least one fixed oil. 6. The composition as recited in claim 5, wherein the at least one fixed is an oil chosen from: castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil. 7. The composition as recited in claim 2, wherein the oils chosen comprise: lilac flower oil; geraniol, and thyme oil. 8. The composition as recited in claim 3, wherein the oils comprise: lilac flower oil; geraniol, and thyme oil. 9. The composition as recited in claim 6, wherein the oils chosen comprise: lilac flower oil, geraniol, thyme oil, and cumin oil. 10. The composition as recited in claim 9, wherein the oils chosen comprise: about 44%o by weight lilac flower oil, about 10% by weight geraniol, about 2% by weight thyme oil, and about 44% by weight cumin oil. 11. The composition as recited in claim 1, wherein the oils chosen comprise: d- limonene, thyme oil, geraniol, α -pinene, and p-cymene. 12. The composition as recited in claim 3, wherein the oils chosen comprise: d- limonene, thyme oil, geraniol, α -pinene, and p-cymene. 13. The composition as recited in claim 11, wherein the oils chosen comprise: about 20% by weight d-limonene, about 20% by weight thyme oil, about 20%> by weight geraniol, about 20 % by weight α -pinene, and about 20% by weight p-cymene. 14. The composition as recited in claim 11, and further comprising phenylacetaldehyde. 15. The composition as recited in claim 12, and further comprising phenylacetaldehyde. 16. The composition as recited in claim 14, wherein the oils chosen comprise: about 10% by weight d-limonene, about 30% by weight thyme oil, about 35% by weight geraniol, about 10% by weight α -pinene, and about 5% by weight phenylacetaldehyde. 17. The composition as recited in claim 2, wherein the oils chosen comprise: geraniol, thyme oil, and lemon grass oil. 18. The composition as recited in claim 3, wherein the oils chosen comprise: geraniol, thyme oil, and lemon grass oil. 19. The composition as recited in claim 17, wherein the oils chosen comprise: about 50% by weight geraniol, about 40% by weight thyme oil, and about 10% by weight lemon grass oil. 20. The composition as recited in claim 2, wherein the oils chosen comprise: d- limonene, lindenol, eugenol, phenylacetaldehyde, and geraniol. 21. The composition as recited in claim 3, wherein the oils chosen comprise: d- limonene, lindenol, eugenol, phenylacetaldehyde, and geraniol. 22. The composition as recited in claim 20, wherein the oils chosen comprise: about 20% by weight d-limonene, about 10% by weight lindenol, about 10%> by weight eugenol, about 10% by weight phenylacetaldehyde, and about 50% by weight geraniol. 23. The composition as recited in claim 2, wherein the oils chosen comprise: d- limonene, geraniol, α -pinene, p-cymene, and phenylacetaldehyde. 24. The composition as recited in claim 3, wherein the oils chosen comprise: d- limonene, geraniol, α -pinene, p-cymene, and phenylacetaldehyde. 25. The composition as recited in claim 23, wherein the oils chosen comprise: about 15% by weight d-limonene, about 50% by weight geraniol, about 15% by weight α- pinene, about 15% by weight p-cymene, and about 5% by weight phenylacetaldehyde. 26. The composition as recited in claim 2, wherein the oils chosen comprise: d- limonene and p-cymene. 27. The composition as recited in claim 2, wherein the oils chosen comprise: d- limonene, p-cymene, and α-pinene. 28. The composition as recited in claim 2, wherein the oils chosen comprise: d- limonene and α-pinene. 29. The composition as recited in claim 2, wherein the oils chosen comprise: d- limonene and thyme oil. 30. The composition as recited in claim 2, wherein the oils chosen comprise: α- pinene and thyme oil. 31. The composition as recited in claim 2, wherein the oils chosen comprise: p- cymene, α-pinene and thyme oil. 32. The composition as recited in claim 2, wherein the oils chosen comprise: p- cymene and α-pinene. 33. The composition as recited in claim 2, wherein the oils chosen comprise: linalool, p-cymene, thyme oil, and α-pinene. 34. The composition as recited in claim 2, wherein the oils chosen comprise: linalool, p-cymene, and α-pinene. 35. The composition as recited in claim 2, wherein the oils chosen comprise: d- limonene, p-cymene, and thymol. 36. The composition as recited in claim 2, wherein the oils chosen comprise: d- limonene, p-cymene, thymol and geraniol. 37. The composition as recited in claim 2, wherein the oils chosen comprise: α- thujone, α-pinene, sabinene, β-pinene, p-cymene and limonene. 38. The composition as recited in claim 3, wherein the oils chosen comprise: α- thujone, α-pinene, sabinene, β-pinene, p-cymene and limonene. 39. The composition as recited in claim 2, wherein the oils chosen comprise: diethyl phthalate, α-terpineol, piperonal, linalool, gamma-terpineol, methyl di- hydrojasmonate, methyl citrate and isopropyl citrate. 40. The composition as recited in claim 3, wherein the oils chosen comprise: diethyl phthalate, α-terpineol, piperonal, linalool, gamma-terpineol, methyl di- hydrojasmonate, methyl citrate and isopropyl citrate. 41. The composition as recited in claim 2, wherein the oils chosen comprise at least two oils chosen from: d-limonene and p-cymene, α-pinene, thyme oil, linalool, thymol, and geraniol. 42. The composition as recited in claim 3, wherein the oils chosen comprise at least two oils chosen from: d-limonene and p-cymene, α-pinene, thyme oil, linalool, thymol, and geraniol. 43. The composition as recited in claim 2, wherein the composition comprises at least two oils chosen from: d-limonene, p-cymene, α -pinene, thyme oil, thymol, geraniol, lemon grass oil, black seed oil, lilac flower oil, mineral oil, and phenylacetaldehyde. 44. The composition as recited in claim 3, wherein the composition comprises at least two oils chosen from: d-limonene, p-cymene, α -pinene, thyme oil, thymol, geraniol, lemon grass oil, black seed oil, lilac flower oil, mineral oil, and phenylacetaldehyde. 45. A composition for controlling insects comprising at least two oils chosen from: Black seed oil, camphene, d-carvone, 1-carvone, 1,8-cineole, isopropyl citrate, lemon grass oil, lilac flower oil, lime oil, linalyl anthranilate, methyl citrate, methyl di- hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonyl, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, gamma-terpineol, 2-tert-butyl-p-quinone, and α-thujone. 46. The composition as recited in claim 45, and further comprising at least one fixed oil. 47. The composition as recited in claim 46, wherein the at least one fixed oil comprises an oil chosen from: castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil. 48. The composition as recited in claim 47, wherein the oils chosen comprise: lilac flower oil and cumin oil. 49. A composition for controlling insects, comprising at least one oil chosen from: black seed oil and lilac flower oil. 50. The composition as recited in claim 49, and further comprising at least one plant essential oil. 51. The composition as recited in claim 50, wherein the at least one plant essential oil comprises an oil chosen from: t-anthole, black seed oil, camphene, carvacrol, d-carvone, 1- carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil, lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p- quinone, α-thujone, thyme oil, thymol, castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil. 52. The composition as recited in claim 51, wherein the oils chosen comprise: lilac flower oil and cumin oil. 53. The composition as recited in claim 51, wherein the oils chosen comprise: lilac flower oil; geraniol, and thyme oil. 54. The composition as recited in claim 53, wherein the oils chosen comprise: lilac flower oil, geraniol, thyme oil, and cumin oil. 55. A composition for controlling insects, comprising: at least one plant essential oil; and an insect control agent, wherein the final concentration of said insect control agent is lower than required to exhibit insect control activity when said insect control agent is used alone. 56. The composition as recited in claim 55, wherein the at least one plant essential oil comprises at least one oil chosen from: t-anthole, black seed oil, camphene, carvacrol, d- carvone, 1-carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil (LFO), lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma- terpineol, 2-tert-butyl-p-quinone, α-thujone, thyme oil, and thymol. 57. The composition as recited in claim 56, wherein the insect control agent is DEET. 58. The composition as recited in claim 55, wherein the insect control agent is DEET. 59. The composition as recited in claim 58, wherein the final concentration of DEET is at least as low as 10%. 60. The composition as recited in claim 59, wherein the final concentration of DEET is at least as low as 5%. 61. The composition as recited in claim 57, and further comprising a fixed oil. 62. The composition as recited in claim 61, wherein the fixed oil comprises an oil chosen from: castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil. 63. The composition as recited in claim 62, wherein the oils chosen comprise: DEET, lilac flower oil, and cumin oil. 64. The composition as recited in claim 63, wherein the oils chosen comprise: about 10% by weight DEET, about 45% by weight lilac flower oil and about 45% by weight cumin oil. 65. The composition as recited in claim 57, wherein the oils chosen comprise: DEET, d-limonene, thyme oil, geraniol, α-pinene, and p-cymene. 66. The composition as recited in claim 65, wherein the oils chosen comprise: about 10% by weight DEET, about 18% by weight d-limonene, about 18% by weight thyme oil, about 20% by weight geraniol, about 20% by weight α-pinene, and about 18% by weight p-cymene. 67. A method for controlling insects, comprising: providing a composition comprising at least two oils chosen from: t-anthole, black seed oil, camphene, carvacrol, d-carvone, 1-carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil, lime oil, d- limonene, linalyl anthranilate, linalool, lindenol, methyl cifrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p-quinone, α-thujone, thyme oil, and thymol.; exposing the insects to a concentration of the composition sufficient to cause a repellant effect. 68. The method as recited in claim 67, wherein the oils chosen comprise: lilac flower oil; geraniol, and thyme oil. 69. The method as recited in claim 67, wherein the oils chosen comprise: α- thujone, α-pinene, sabinene, β-pinene, p-cymene and limonene. 70. The method as recited in claim 67, wherein the oils chosen comprise: diethyl phthalate, α-terpineol, piperonal, linalool, gamma-terpineol, methyl di-hydrojasmonate, methyl citrate and isopropyl citrate. 71. A method for controlling insects, comprising: providing a composition comprising at least one oil chosen from: black seed oil and lilac flower oil.; exposing the insects to a concentration of the composition sufficient to cause a repellant effect. 72. The method as recited in claim 71, wherein the composition provided further comprises at least one additional plant essential oil. 73. A method for controlling insects, comprising: providing a composition as recited in claim 52; exposing the insects to a concentration of the composition sufficient to cause a repellant effect. 74. The method as recited in claim 73, wherein the insect control agent of the composition provided is DEET. 75. A method for controlling insects, comprising: providing a composition comprising at least two oils chosen from: t-anthole, black seed oil, camphene, carvacrol, d-carvone, 1-carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil (LFO), lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di- hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p-quinone, α-thujone, thyme oil, and thymol; and exposing the insects to a concentration of the composition sufficient to cause a pesticidal effect. 76. A method for controlling insects, comprising: providing a composition comprising at least one plant essential oil; and an insect control agent, wherein the final concentration of said insect control agent is lower than required to exhibit insect control activity when said insect control agent is used alone; and exposing the insects to a concentration of the composition sufficient to cause a repellant effect. 77. The method as recited in claim 76, wherein the insect control agent of the composition provided is DEET. 78. The method as recited in claim 77, wherein the at least one plant essential oil comprises an oil chosen from: t-anthole, black seed oil, camphene, carvacrol, d-carvone, 1- carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil (LFO), lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α- pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p- quinone, α-thujone, thyme oil, and thymol. 79. A method of screening compositions for insect control activity, comprising: providing insect cells expressing a receptor chosen from: tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor; adding said compositions to the cells; measuring the binding affinity of said compositions to the receptor; and selecting compositions having an affinity for the receptor. 80. A method of screening compositions for insect control activity, comprising: providing insect cells expressing a receptor chosen from: tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor; adding said compositions to the cells; extracting intracellular cAMP or Ca2+ from the cells; measuring the intracellular cAMP or Ca2+ levels; comparing the intracellular cAMP or Ca2+ levels in cells treated with said compositions to the intracellular cAMP or Ca2+ levels in untreated cells; and selecting compositions, the treatment with which, causes a change in intracellular cAMP, Ca2+ or both. 81. A report produced by the method, comprising: providing insect cells expressing a receptor chosen from: tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor; adding said compositions to the cells; measuring the binding affinity of said compositions to the receptor; and selecting compositions having an affinity for the receptor. 82. A report produced by the method, comprising: providing insect cells expressing a receptor chosen from: tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor; adding said compositions to the cells; extracting intracellular cAMP or Ca2+ from the cells; measuring the intracellular cAMP or Ca2+ levels; comparing the intracellular cAMP or Ca2+ levels in cells treated with said compositions to the intracellular cAMP or Ca2+ levels in untreated cells; and selecting compositions, the treatment with which, causes a change in intracellular cAMP, Ca2+ or both. 83. A strain of Drosophila Schneider comprising a nucleic acid sequence as set forth in Figures 31 A and 3 IB for a tyramine receptor. 84. A strain of Drosophila Schneider comprising a nucleic acid sequence as set forth in Figures 32A and 32B for a Or43a Olfactory receptor. 85. A strain of Drosophila Schneider comprising a nucleic acid sequence as set forth in Figures 33A and 33B for a Or83b Olfactory receptor.
法律状态
(WO2004100971) LEGAL DETAILS FOR WO2004100971  Actual or expected expiration date=2007-06-24    Legal state=DEAD    Status=LAPSED     Event publication date=2004-04-26  Event code=WO/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=WO WOUS2004012947  Application date=2004-04-26  Standardized application number=2004WO-US12947     Event publication date=2004-11-25  Event code=WO/A1  Event type=Examination events  Published application with search report  Publication country=WO  Publication number=WO2004100971  Publication stage Code=A1  Publication date=2004-11-25  Standardized publication number=WO2004100971     Event publication date=2004-11-25  Event code=WO/AL  Event indicator=Pos  Event type=Designated states  Designated countries for regional patents BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG    Event publication date=2004-11-25  Event code=WO/AK  Event indicator=Pos  Event type=Designated states  Designated states AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW    Event publication date=2007-06-24  Event code=WO/EETL  Event type=Event indicating Not In Force  PCT Application validity period expired. 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