一种​柿子​果实​色素​的​制备​方法

源语言标题
(CN104693834B) 一种​柿子​果实​色素​的​制备​方法
公开号/公开日
CN104693834 A 2015-06-10 [CN104693834]CN104693834 B 2017-05-17 [CN104693834B] / 2015-06-102017-05-17
申请号/申请日
2015CN-0147263 / 2015-03-31
发明人
LIU XIAOFENG;HE MINGXI;LUO ZHISONG;LI JINGSHENG;LIU ERWEI;
申请人
GUILIN DEKUN BIOTECHNOLOGY;
主分类号
IPC分类号
C09B-061/00C09B-067/54
摘要
(CN104693834B) 本​发明​公开​了​种​柿子​果实​色素​的​制备​方法,包括​下述​步骤:原料​处理、高压​微​射流​超微​粉碎​处理、发酵​处理、萃取、大​孔​树脂​柱​层​析、聚酰胺​柱​层​析、葡​聚糖​凝胶​柱​层​析​对​色素​液​进行​吸附​纯化、采用​制备​型​液相​色谱​进行​柿子​果实​色素​的​分离​纯化,能​有效​除去​色素​液​中​水溶性​杂质,实现​色素​单体​的​初步​分离,能够​有效​实现​柿子​果实​色素​单体​的​分离,保证​了​产品​的​纯度。
地址
代理人
代理机构
;
优先权号
2015CN-0147263 2015-03-31
主权利要求
(CN104693834B) 1. One method for producing fruit pigment persimon, characterized in, including the steps of: 1) the feedstock processing: will persimon cleaning, crushing; 2) high-pressure micro jet micronization processing: will be crushed persimon, in accordance with 1:5-6 the weight ratio of purified water was added, with a high shear emulsifier processing 10-20min, a rotational speed of 8000-12000r/min, and then processed using a high pressure jet superfine grinding device for 25 Ć, the treatment pressure is 80-120 mpa, processing times of 2-3 times, to yield a cream slurry; 3) fermentation: the step 2) to obtain a paste-like slurry the ultra-high instantaneous sterilization, and then adjusting pH 6.5-7.0, in volume percent to 10-15% of lactic acid bacteria culture fluid species mature access, into the fermenter fermentation, the fermentation temperature at 35-37 Ć, fermentation time 3-4h; 4) extraction: step 3) obtained by the ultra-high instantaneous sterilization fermentation broth, with an equal volume of ethyl acetate was repeatedly extracted from the fermentation broth, the aqueous layer, inacuo to remove ethyl acetate, ethyl acetate layer was repeated several times until a colorless; 5) a macro porous resin absorption purification: selected HP-20 macro porous resin, a method of a wet pretreated column, pH 3.5 with dilute hydrochloric acid to su liquid toner, with a constant flow pump control pigment solution through adsorption column, to be out of the liquid absorbance of the sample liquid was stopped after 10% absorbance was on the pigment solution, washed with pure water several times to wash the resin surface, 90% of the mass fraction of from 3.5 to pH of the solution to ethanol as eluent to pigment solution, liquid vacuum concentrated to remove ethanol the obtained dye, the dye solution to obtain a preliminarily purified; 6) polyamide column adsorption purification: polyamide pretreated wet column packed, step 5) to obtain pigment solution was passed through a preliminary purification polyamide column, mass fraction of from 50% to 0.1% of the mass fractions of methanol and formic acid are mixed solution as the eluent, collecting the colored portion, the eluent was concentrated in vacuo; 7) Sephadexs column purification: Sephadexs pretreated wet column packed, step 6) to obtain a purified sample of over Sephadex column, 50% by mass fraction of 0.1% formic acid in methanol and the mass fractions of mixed solution as the eluent, collecting the colored portion, the eluent was concentrated in vacuo; 8) preparative liquid chromatography separation and refinement: using preparative liquid chromatography separation and purification after the concentration of the pigment solution, to obtain persimon fruit pigment solution; 9) dry: freeze-dried, removing an aqueous pigment solution, to obtain persimon fruit pigment.
法律状态
(CN104693834B) LEGAL DETAILS FOR CN104693834  Actual or expected expiration date=2035-03-31    Legal state=ALIVE    Status=GRANTED     Event publication date=2015-03-31  Event code=CN/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=CN CN201510147263  Application date=2015-03-31  Standardized application number=2015CN-0147263     Event publication date=2015-06-10  Event code=CN/A  Event type=Examination events  Published application  Publication country=CN  Publication number=CN104693834  Publication stage Code=A  Publication date=2015-06-10  Standardized publication number=CN104693834     Event publication date=2015-06-10  Event code=CN/C06  Event indicator=Pos  Event type=Examination events  Publication    Event publication date=2015-07-08  Event code=CN/C10  Event type=Examination events  Request of examination as to substance    Event publication date=2017-05-17  Event code=CN/B  Event indicator=Pos  Event type=Event indicating In Force  Granted patent for invention  Publication country=CN  Publication number=CN104693834  Publication stage Code=B  Publication date=2017-05-17  Standardized publication number=CN104693834B     Event publication date=2017-05-17  Event code=CN/GR01  Event indicator=Pos  Event type=Event indicating In Force  PATENT GRANT
专利类型码
AB
国别省市代码
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