Antibiotic susceptibility testing using probes for preribosomal rna 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2015104789 A1 2015-04-16 [US20150104789] / 2015-04-16
申请号/申请日
2013US-14398725 / 2013-05-03
发明人
HAAKE DAVID A;HALFORD COLIN;BABBITT JANE T;CHURCHILL BERNARD M;
申请人
UNIVERSITY OF CALIFORNIA;UNIVERSITY OF CALIFORNIA LOS ANGELES;US DEPARTMENT OF VETERANS AFFAIRS;
主分类号
IPC分类号
C12Q-001/68
摘要
(US20150104789) Described are probes and methods for detecting antibiotic susceptibility of a specimen.  The method comprises contacting the specimen with an oligonucleotide probe that specifically hybridizes with a target nucleic acid sequence region of ribosomal RNA.  The target sequence is at the junction between a pre-ribosomal RNA tail and mature ribosomal RNA of 23S or 16S rRNA.  Performing the method in the presence and absence of an antibiotic permits detection of antibiotic susceptibility.
机翻摘要
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地址
代理人
代理机构
;
优先权号
2012US-61643009 2012-05-04 2013US-14398725 2013-05-03 2013WO-US39574 2013-05-03
主权利要求
(US20150104789) 1. A method for determining whether a sample of bacteria is susceptible to an antibiotic agent, the method comprising the steps of: (a) contacting a specimen obtained from the sample with an oligonucleotide probe or pair of probes in the absence of the agent, wherein the probe or pair of probes specifically hybridizes to a target sequence over the full length of the target sequence, wherein the target sequence comprises 25-35 contiguous nucleotides of bacterial ribosomal RNA (rRNA) spanning a splice site between a pre-ribosomal RNA (prRNA) tail and mature ribosomal RNA (mrRNA); (b) contacting a specimen obtained from the sample with the probe or pair of probes in the presence of the antibiotic agent; (c) detecting the relative amounts of probe hybridization to the target sequence in the specimens of (a) and (b); (d) identifying the sample as susceptible to antibiotic treatment if the amount of probe hybridization to the target sequence in step (b) is reduced by at least 80% relative to the amount of probe hybridization to the target sequence in step (a). 14. A method for determining the antibiotic efficacy of a candidate antibiotic agent, the method comprising the steps of: (a) contacting a specimen obtained from the sample with an oligonucleotide probe or pair of probes in the absence of the agent, wherein the probe or pair of probes specifically hybridizes to a target sequence over the full length of the target sequence, wherein the target sequence comprises 25-35 contiguous nucleotides of bacterial ribosomal RNA (rRNA) spanning a splice site between pre-ribosomal RNA (prRNA) tail and mature ribosomal RNA (mrRNA); (b) contacting a specimen obtained from the sample with the probe or pair of probes in the presence of the agent; (c) detecting the relative amounts of probe hybridization to the target sequence in the specimens of (a) and (b); (d) identifying the agent as effective if the amount of probe hybridization to the target sequence in step (b) is reduced by at least 80% relative to the amount of probe hybridization to the target sequence in step (a). 15. A device for detecting pre-rRNA in a bacterial sample, the device comprising an oligonucleotide probe immobilized on a solid support, wherein the oligonucleotide probe is between about 10 to 50 nucleotides in length and is capable of selectively hybridizing to a target sequence over the full length of the target sequence, wherein the target sequence comprises 25-35 contiguous nucleotides of bacterial ribosomal RNA (rRNA) spanning a splice site between pre-ribosomal RNA (prRNA) tail and mature ribosomal RNA (mrRNA). 17. A kit comprising a pair of oligonucleotide probes selected from: (a) E. coli (all enterobacteriaceae) probes:(SEQ ID NO: 5)5'-AAGCCTCACGGTTCATTand(SEQ ID NO: 6)GGCGTTGTAAGGTT;(b) Pseudomonas aeruginosa probes:(SEQ ID NO: 7)5'-AAGCCTCACGGGCAATTand(SEQ ID NO: 8)GGTGTTATATGGTC;(c) Streptococcus pyogenes probes:(SEQ ID NO: 9)AAGTCCTCGAGCTATTand(SEQ ID NO: 10)ATTTCTTTTTGGAT;and(d) Staphylococcus aureus probes(SEQ ID NO: 11)AAGTCTTCGATCGATTand(SEQ ID NO: 12)CATTTATTTTGATT. 18. A method for monitoring the growth rate of a bacterial culture, the method comprising: (a) contacting a specimen obtained from the culture with a probe or pair of probes that specifically hybridizes to a target sequence over the full length of the target sequence, wherein the target sequence comprises 25-35 contiguous nucleotides of bacterial ribosomal RNA (rRNA) spanning a splice site between pre-ribosomal RNA (prRNA) tail and mature ribosomal RNA (mrRNA); (b) detecting the amount of probe hybridization to the target sequence in the specimen of (a) relative to an earlier time point or other control. (c) identifying the culture as growing if the amount of probe hybridization to the target sequence in step (b) is increasing relative to the amount of probe hybridization to the target sequence at the earlier time point.
法律状态
(US20150104789) LEGAL DETAILS FOR US2015104789  Actual or expected expiration date=2033-05-03    Legal state=ALIVE    Status=PENDING     Event publication date=2013-05-03  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US14398725  Application date=2013-05-03  Standardized application number=2013US-14398725     Event publication date=2013-05-03  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: JOHANNSEN, DIANA B    Event publication date=2013-05-03  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1634     Event publication date=2013-05-03  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: UCLA.199USWO Customer Nbr: 59612    Event publication date=2013-05-03  Event code=US/ENT  Event type=Administrative notifications  Business Entity Status: UNDISCOUNTED    Event publication date=2013-05-03  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=Yes     Event publication date=2014-11-03  Event code=US/PTARDY  Event indicator=Pos  Event type=Examination events  Patent Term Adjustment - Ready for Examination    Event publication date=2014-11-03  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2014-11-03  Event code=US/ENTYSTA  Event type=Administrative notifications  Entity Status Set to Undiscounted    Event publication date=2014-11-05  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, CALIF  Effective date of the event=2013-07-15  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:HAAKE, DAVID A. HALFORD, COLIN BABBITT, JANE T. REEL/FRAME:034112/0285     Event publication date=2014-11-05  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, CALIF  Effective date of the event=2013-09-23  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNOR:CHURCHILL, BERNARD M. REEL/FRAME:034112/0271     Event publication date=2014-11-05  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: THE UNITED STATES GOVERNMENT REPRESENTED BY THE DE  Effective date of the event=2013-07-15  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:HAAKE, DAVID A. HALFORD, COLIN BABBITT, JANE T. REEL/FRAME:034112/0285     Event publication date=2015-01-08  Event code=US/APPFILEREC  Event type=Administrative notifications  Event type=OAO  Filing Receipt    Event publication date=2015-01-08  Event code=US/M903  Event indicator=Pos  Event type=Examination events  Event type=OAO  Notice of DO/EO Acceptance Mailed    Event publication date=2015-01-21  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner in GAU    Event publication date=2015-04-16  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2015104789  Publication stage Code=A1  Publication date=2015-04-16  Standardized publication number=US20150104789     Event publication date=2015-04-16  Event code=US/PGPUBN  Event indicator=Pos  Event type=Examination events  Event type=OAO  PG-Pub Issue Notification    Event publication date=2015-05-05  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF  Effective date of the event=2015-04-21  CONFIRMATORY LICENSE ASSIGNOR:UNIVERSITY OF CALIFORNIA LOS ANGELES REEL/FRAME:035583/0564     Event publication date=2016-02-23  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2017-05-15  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2017-08-23  Event code=US/RECEIPTCOR  Event type=Administrative notifications  Event type=OAO  Filing Receipt - Corrected
专利类型码
A1
国别省市代码
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