Highly efficient preparation of free all-trans-astaxanthin from Haematococcus pluvialis extract by a rapid biocatalytic method based on crude extracellular enzyme extract 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
International Journal of Food Science and Technology
年/卷/期
2019 / 54 / 2
页码
376-386
ISSN号
0950-5423
作者单位
Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China;Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China;Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China;Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China;CNR, Ist Chim Riconoscimento Mol, V Mario Bianco 9, I-20131 Milan, Italy;Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China;Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China;Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China;
作者
Gao, Xinwei;Sun, Jianan;Liu, Zhen;Huang, Wen-Can;Secundo, Francesco;Zhao, Yuanhui;Xue, Changhu;Mao, Xiangzhao;
摘要
A highly efficient, rapid, green and safe procedure for the preparation of free all-trans-astaxanthin from Haematococcus pluvialis algal extract, by a crude extracellular enzyme extract, was reported. The free all-trans-astaxanthin obtained by the biocatalysed method had fewer side products compared to the saponification procedure. Through single-factor experiments and a Box-Behnken design, it was possible to find the optimal biocatalytic conditions for the hydrolysis of 2 mg of H. pluvialis oil with 14.7 mg (protein content) of lyophilised crude extracellular enzyme extract obtained from Pseudomonas aeruginosa. The reaction was carried out in 30 min at pH 9.16 and 36 degrees C, in 5.5 mL total reaction volume, under nitrogen atmosphere and dark conditions. The hydrolysis ratio of the astaxanthin esters was 98.72%, and the production of free all-trans-astaxanthin was 82.83 mu g per mg of H. pluvialis oil. The method herein reported was simpler than other enzymatic methods previously described and allowed saving of time and costs.
机翻摘要
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关键词/主题词
Esterase;free all-trans-astaxanthin;Haematococcus pluvialis;lipase;Pseudomonas aeruginosa;
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