Optimization of the organic solvent-stable asymmetric hydrogen transfer system of Rhodococcus ruber DSM 44541: an activity-growth study 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
Journal of molecular catalysis, B. Enzymatic
年/卷/期
2003 / 22 / 1/2
页码
1-6
ISSN号
1381-1177
作者单位
Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, Graz A-8010, Austria;
作者
B.Kosjek;W.Stampfer;S.M.Glueck;M.Pogorevc;U.Ellmer;S.R.Wallner;M.F.Koegl;T.M.Poessl;S.F.Mayer;B.Ueberbacher;K.Faber;W.Kroutil;
摘要
The organic solvent-stable redox-system of Rhodococcus ruber DSM 44541, which allows the efficient oxidation/reductioin of sec-alcohol/ketones at the expense of acetone/2-propanol, respectively, as cosubstrate was optimized with respect to a maximum of alcohol dehydrogenase activity during cell growth. Comparison of the fermentation of R. ruber DSM 44541 in shake flasks cultures (11 flask with 250 ml medium) and in a bioreactor (151 with 101 working volume) revealed that the desired organic solvent-stable alcohol dehydrogenase activity reached its maximum during the log phase for the bioreactor. In contrast, in shake flasks the maximum of activity was reached during the stationary phase.
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关键词/主题词
rhodococcus ruber;sec-alcohol dehydrogenase;fermentation;activity-growth;
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